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P9WP28

- DAGK_MYCTO

UniProt

P9WP28 - DAGK_MYCTO

Protein

Diacylglycerol kinase

Gene

dagK

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 5 (01 Oct 2014)
      Sequence version 1 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derived from phosphatidic acid. Is also able to phosphorylate other various amphipathic lipids of host and bacterial origin in vitro, such as ceramide.1 Publication

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

    Cofactori

    Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei101 – 1011ATPPROSITE-ProRule annotation
    Metal bindingi226 – 2261Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi229 – 2291MagnesiumBy similarity
    Metal bindingi231 – 2311Magnesium; via carbonyl oxygenBy similarity
    Active sitei285 – 2851Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 235ATPPROSITE-ProRule annotation
    Nucleotide bindingi76 – 827ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. phospholipid biosynthetic process Source: UniProtKB-KW
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase (EC:2.7.1.107)
    Short name:
    DAG kinase
    Short name:
    DAGK
    Gene namesi
    Name:dagK
    Ordered Locus Names:MT2312
    OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
    Taxonomic identifieri83331 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001020: Chromosome

    Subcellular locationi

    Secretedcell wall Curated

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell wall, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene exhibit a disruption of the production of certain higher-order phosphatidylinositol mannosides (PIMs) species.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Diacylglycerol kinasePRO_0000427024Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP9WP28.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 140132DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the diacylglycerol/lipid kinase family.Curated
    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR005218. Diacylglycerol/lipid_kinase.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view]
    PfamiPF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P9WP28-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAGQLRRHE IGKVTALTNP LSGHGAAVKA AHGAIARLKH RGVDVVEIVG    50
    GDAHDARHLL AAAVAKGTDA VMVTGGDGVV SNALQVLAGT DIPLGIIPAG 100
    TGNDHAREFG LPTKNPKAAA DIVVDGWTET IDLGRIQDDN GIEKWFGTVA 150
    ATGFDSLVND RANRMRWPHG RMRYYIAMLA ELSRLRPLPF RLVLDGTEEI 200
    VADLTLADFG NTRSYGGGLL ICPNADHSDG LLDITMAQSD SRTKLLRLFP 250
    TIFKGAHVEL DEVSTTRAKT VHVECPGINV YADGDFACPL PAEISAVPAA 300
    LQVLRPRHG 309
    Length:309
    Mass (Da):32,805
    Last modified:April 16, 2014 - v1
    Checksum:i34F5E363D62BB284
    GO

    Sequence cautioni

    The sequence AE000516 differs from that shown. Reason: Frameshift at position 95.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000516 Genomic DNA. No translation available.
    PIRiH70861.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000516 Genomic DNA. No translation available.
    PIRi H70861.

    3D structure databases

    ProteinModelPortali P9WP28.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR005218. Diacylglycerol/lipid_kinase.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CDC 1551 / Oshkosh.
    2. "M. tuberculosis Rv2252 encodes a diacylglycerol kinase involved in the biosynthesis of phosphatidylinositol mannosides (PIMs)."
      Owens R.M., Hsu F.F., VanderVen B.C., Purdy G.E., Hesteande E., Giannakas P., Sacchettini J.C., McKinney J.D., Hill P.J., Belisle J.T., Butcher B.A., Pethe K., Russell D.G.
      Mol. Microbiol. 60:1152-1163(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
      Strain: CDC 1551 / Oshkosh.

    Entry informationi

    Entry nameiDAGK_MYCTO
    AccessioniPrimary (citable) accession number: P9WP28
    Secondary accession number(s): L0TAM7, O53526
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 5 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3