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P9WP28

- DAGK_MYCTO

UniProt

P9WP28 - DAGK_MYCTO

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Protein
Diacylglycerol kinase
Gene
dagK, MT2312
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derived from phosphatidic acid. Is also able to phosphorylate other various amphipathic lipids of host and bacterial origin in vitro, such as ceramide.1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Cofactori

Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011ATP By similarity
Metal bindingi226 – 2261Magnesium; via carbonyl oxygen By similarity
Metal bindingi229 – 2291Magnesium By similarity
Metal bindingi231 – 2311Magnesium; via carbonyl oxygen By similarity
Active sitei285 – 2851Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 235ATP By similarity
Nucleotide bindingi76 – 827ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: InterPro
  3. diacylglycerol kinase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phospholipid biosynthetic process Source: UniProtKB-KW
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Ordered Locus Names:MT2312
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001020: Chromosome

Subcellular locationi

Secretedcell wall Reviewed prediction 1 Publication

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene exhibit a disruption of the production of certain higher-order phosphatidylinositol mannosides (PIMs) species.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Diacylglycerol kinase
PRO_0000427024Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP9WP28.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 140132DAGKc
Add
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.

Family and domain databases

InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WP28-1 [UniParc]FASTAAdd to Basket

« Hide

MSAGQLRRHE IGKVTALTNP LSGHGAAVKA AHGAIARLKH RGVDVVEIVG    50
GDAHDARHLL AAAVAKGTDA VMVTGGDGVV SNALQVLAGT DIPLGIIPAG 100
TGNDHAREFG LPTKNPKAAA DIVVDGWTET IDLGRIQDDN GIEKWFGTVA 150
ATGFDSLVND RANRMRWPHG RMRYYIAMLA ELSRLRPLPF RLVLDGTEEI 200
VADLTLADFG NTRSYGGGLL ICPNADHSDG LLDITMAQSD SRTKLLRLFP 250
TIFKGAHVEL DEVSTTRAKT VHVECPGINV YADGDFACPL PAEISAVPAA 300
LQVLRPRHG 309
Length:309
Mass (Da):32,805
Last modified:April 16, 2014 - v1
Checksum:i34F5E363D62BB284
GO

Sequence cautioni

The sequence AE000516 differs from that shown. Reason: Frameshift at position 95.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000516 Genomic DNA. No translation available.
PIRiH70861.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000516 Genomic DNA. No translation available.
PIRi H70861.

3D structure databases

ProteinModelPortali P9WP28.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view ]
Pfami PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
PROSITEi PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CDC 1551 / Oshkosh.
  2. "M. tuberculosis Rv2252 encodes a diacylglycerol kinase involved in the biosynthesis of phosphatidylinositol mannosides (PIMs)."
    Owens R.M., Hsu F.F., VanderVen B.C., Purdy G.E., Hesteande E., Giannakas P., Sacchettini J.C., McKinney J.D., Hill P.J., Belisle J.T., Butcher B.A., Pethe K., Russell D.G.
    Mol. Microbiol. 60:1152-1163(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: CDC 1551 / Oshkosh.

Entry informationi

Entry nameiDAGK_MYCTO
AccessioniPrimary (citable) accession number: P9WP28
Secondary accession number(s): L0TAM7, O53526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: July 9, 2014
This is version 4 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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