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Protein

Coenzyme A biosynthesis bifunctional protein CoaBC

Gene

coaBC

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine (By similarity).By similarity

Miscellaneous

Was identified as a high-confidence drug target.

Catalytic activityi

N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2.
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase (coaA), Type III pantothenate kinase (coaX), Pantothenate kinase (coaA), Type III pantothenate kinase (coaX)
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (LH57_07625), Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (LH57_07625), Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  4. Phosphopantetheine adenylyltransferase (coaD), Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE), Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei285CTPBy similarity1
Binding sitei295CTPBy similarity1
Binding sitei354CTPBy similarity1
Binding sitei358CTPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • coenzyme A biosynthetic process Source: MTBBASE
  • growth Source: MTBBASE
  • pantothenate catabolic process Source: InterPro

Keywordsi

Molecular functionDecarboxylase, Ligase, Lyase
LigandFlavoprotein, FMN

Enzyme and pathway databases

UniPathwayiUPA00241; UER00353.
UPA00241; UER00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme A biosynthesis bifunctional protein CoaBC
Alternative name(s):
DNA/pantothenate metabolism flavoprotein
Including the following 2 domains:
Phosphopantothenoylcysteine decarboxylase (EC:4.1.1.36)
Short name:
PPCDC
Alternative name(s):
CoaC
Phosphopantothenate--cysteine ligase (EC:6.3.2.5)
Alternative name(s):
CoaB
PPC synthetase
Short name:
PPCS
Phosphopantothenoylcysteine synthase
Gene namesi
Name:coaBC
Synonyms:dfp
Ordered Locus Names:Rv1391
ORF Names:MTCY21B4.08
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1391.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: MTBBASE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002326941 – 418Coenzyme A biosynthesis bifunctional protein CoaBCAdd BLAST418

Proteomic databases

PaxDbiP9WNZ1.

Interactioni

Subunit structurei

Homododecamer, the CoaB domains form homodimers.

Protein-protein interaction databases

STRINGi83332.Rv1391.

Structurei

3D structure databases

ProteinModelPortaliP9WNZ1.
SMRiP9WNZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 193Phosphopantothenoylcysteine decarboxylaseAdd BLAST193
Regioni194 – 418Phosphopantothenate--cysteine ligaseAdd BLAST225

Sequence similaritiesi

In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.Curated
In the C-terminal section; belongs to the PPC synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJS. Bacteria.
COG0452. LUCA.
KOiK13038.
OMAiVRFIGNH.
PhylomeDBiP9WNZ1.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
3.40.50.1950. 1 hit.
InterProiView protein in InterPro
IPR035929. CoaB-like_sf.
IPR005252. CoaBC.
IPR007085. DNA/pantothenate-metab_flavo_C.
IPR036551. Flavin_trans-like.
IPR003382. Flavoprotein.
PfamiView protein in Pfam
PF04127. DFP. 1 hit.
PF02441. Flavoprotein. 1 hit.
SUPFAMiSSF102645. SSF102645. 1 hit.
SSF52507. SSF52507. 1 hit.
TIGRFAMsiTIGR00521. coaBC_dfp. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WNZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDHKRIPKQ VIVGVSGGIA AYKACTVVRQ LTEASHRVRV IPTESALRFV
60 70 80 90 100
GAATFEALSG EPVCTDVFAD VPAVPHVHLG QQADLVVVAP ATADLLARAA
110 120 130 140 150
AGRADDLLTA TLLTARCPVL FAPAMHTEMW LHPATVDNVA TLRRRGAVVL
160 170 180 190 200
EPATGRLTGA DSGAGRLPEA EEITTLAQLL LERHDALPYD LAGRKLLVTA
210 220 230 240 250
GGTREPIDPV RFIGNRSSGK QGYAVARVAA QRGADVTLIA GHTAGLVDPA
260 270 280 290 300
GVEVVHVSSA QQLADAVSKH APTADVLVMA AAVADFRPAQ VATAKIKKGV
310 320 330 340 350
EGPPTIELLR NDDVLAGVVR ARAHGQLPNM RAIVGFAAET GDANGDVLFH
360 370 380 390 400
ARAKLRRKGC DLLVVNAVGE GRAFEVDSND GWLLASDGTE SALQHGSKTL
410
MASRIVDAIV TFLAGCSS
Length:418
Mass (Da):43,577
Last modified:April 16, 2014 - v1
Checksum:i48BD95E536595506
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44150.1.
PIRiE70899.
RefSeqiNP_215907.1. NC_000962.3.
WP_003898859.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44150; CCP44150; Rv1391.
GeneIDi886749.
KEGGimtu:Rv1391.
mtv:RVBD_1391.
PATRICifig|83332.111.peg.1550.

Similar proteinsi

Entry informationi

Entry nameiCOABC_MYCTU
AccessioniPrimary (citable) accession number: P9WNZ1
Secondary accession number(s): L0T847, P67733, P71661
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 25, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families