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Protein

Chromosomal replication initiator protein DnaA

Gene

dnaA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity). Binds its own promoter.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi208 – 2158ATPSequence analysis

GO - Molecular functioni

  • ATPase activity Source: MTBBASE
  • ATP binding Source: MTBBASE
  • DNA replication origin binding Source: MTBBASE

GO - Biological processi

  • ADP biosynthetic process Source: MTBBASE
  • dephosphorylation Source: MTBBASE
  • DNA replication initiation Source: GO_Central
  • regulation of DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosomal replication initiator protein DnaA
Gene namesi
Name:dnaA
Ordered Locus Names:Rv0001
ORF Names:MTV029.01
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0001.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507Chromosomal replication initiator protein DnaAPRO_0000114218Add
BLAST

Proteomic databases

PaxDbiP9WNW3.

Expressioni

Inductioni

Expression depends on the two-component regulatory system MtrA/MtrB.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0001.

Structurei

3D structure databases

ProteinModelPortaliP9WNW3.
SMRiP9WNW3. Positions 166-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaA family.Curated

Phylogenomic databases

eggNOGiENOG4105CI4. Bacteria.
COG0593. LUCA.
KOiK02313.
OMAiEQSEYNT.
PhylomeDBiP9WNW3.

Family and domain databases

Gene3Di1.10.1750.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00377. DnaA_bact.
InterProiIPR003593. AAA+_ATPase.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013317. DnaA.
IPR013159. DnaA_C.
IPR027417. P-loop_NTPase.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
[Graphical view]
PRINTSiPR00051. DNAA.
SMARTiSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48295. SSF48295. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00362. DnaA. 1 hit.
PROSITEiPS01008. DNAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WNW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDDPGSGFT TVWNAVVSEL NGDPKVDDGP SSDANLSAPL TPQQRAWLNL
60 70 80 90 100
VQPLTIVEGF ALLSVPSSFV QNEIERHLRA PITDALSRRL GHQIQLGVRI
110 120 130 140 150
APPATDEADD TTVPPSENPA TTSPDTTTDN DEIDDSAAAR GDNQHSWPSY
160 170 180 190 200
FTERPHNTDS ATAGVTSLNR RYTFDTFVIG ASNRFAHAAA LAIAEAPARA
210 220 230 240 250
YNPLFIWGES GLGKTHLLHA AGNYAQRLFP GMRVKYVSTE EFTNDFINSL
260 270 280 290 300
RDDRKVAFKR SYRDVDVLLV DDIQFIEGKE GIQEEFFHTF NTLHNANKQI
310 320 330 340 350
VISSDRPPKQ LATLEDRLRT RFEWGLITDV QPPELETRIA ILRKKAQMER
360 370 380 390 400
LAVPDDVLEL IASSIERNIR ELEGALIRVT AFASLNKTPI DKALAEIVLR
410 420 430 440 450
DLIADANTMQ ISAATIMAAT AEYFDTTVEE LRGPGKTRAL AQSRQIAMYL
460 470 480 490 500
CRELTDLSLP KIGQAFGRDH TTVMYAQRKI LSEMAERREV FDHVKELTTR

IRQRSKR
Length:507
Mass (Da):56,567
Last modified:April 16, 2014 - v1
Checksum:iC4F252150D453A00
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti363 – 3631S → R in AAA85543 (PubMed:7557482).Curated
Sequence conflicti371 – 3711E → V in AAA85543 (PubMed:7557482).Curated
Sequence conflicti381 – 3811A → P in AAA85543 (PubMed:7557482).Curated
Sequence conflicti429 – 4302EE → GR in AAA85543 (PubMed:7557482).Curated
Sequence conflicti448 – 4481M → V in AAB38528 (Ref. 1) Curated
Sequence conflicti458 – 4581S → P in AAA85543 (PubMed:7557482).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38891 Genomic DNA. Translation: AAB38528.2.
X92504 Genomic DNA. Translation: CAA63257.1.
AL123456 Genomic DNA. Translation: CCP42723.1.
U19184 Genomic DNA. Translation: AAA85543.1.
PIRiS70982.
RefSeqiNP_214515.1. NC_000962.3.
WP_003400253.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP42723; CCP42723; Rv0001.
GeneIDi885041.
KEGGimtu:Rv0001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38891 Genomic DNA. Translation: AAB38528.2.
X92504 Genomic DNA. Translation: CAA63257.1.
AL123456 Genomic DNA. Translation: CCP42723.1.
U19184 Genomic DNA. Translation: AAA85543.1.
PIRiS70982.
RefSeqiNP_214515.1. NC_000962.3.
WP_003400253.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliP9WNW3.
SMRiP9WNW3. Positions 166-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv0001.

Proteomic databases

PaxDbiP9WNW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP42723; CCP42723; Rv0001.
GeneIDi885041.
KEGGimtu:Rv0001.

Organism-specific databases

TubercuListiRv0001.

Phylogenomic databases

eggNOGiENOG4105CI4. Bacteria.
COG0593. LUCA.
KOiK02313.
OMAiEQSEYNT.
PhylomeDBiP9WNW3.

Family and domain databases

Gene3Di1.10.1750.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00377. DnaA_bact.
InterProiIPR003593. AAA+_ATPase.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013317. DnaA.
IPR013159. DnaA_C.
IPR027417. P-loop_NTPase.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
[Graphical view]
PRINTSiPR00051. DNAA.
SMARTiSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48295. SSF48295. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00362. DnaA. 1 hit.
PROSITEiPS01008. DNAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The dnaA gene region of M. tuberculosis."
    Qin M.H., Madiraju M.V., Rajagopalan M.
    Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis."
    Salazar L., Fsihi H., De Rossi E., Riccardi G., Rios C., Cole S.T., Takiff H.E.
    Mol. Microbiol. 20:283-293(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  4. "Amplification and cloning of the Mycobacterium tuberculosis dnaA gene."
    Rajagopalan M., Qin M.H., Steingrube V.A., Nash D.R., Wallace R.J. Jr., Madiraju M.V.V.S.
    Gene 163:75-79(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 213-472.
  5. "Modulation of Mycobacterium tuberculosis proliferation by MtrA, an essential two-component response regulator."
    Fol M., Chauhan A., Nair N.K., Maloney E., Moomey M., Jagannath C., Madiraju M.V., Rajagopalan M.
    Mol. Microbiol. 60:643-657(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, DNA-BINDING.
    Strain: ATCC 25618 / H37Rv.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiDNAA_MYCTU
AccessioniPrimary (citable) accession number: P9WNW3
Secondary accession number(s): L0T412
, P49993, P95309, Q59585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 17, 2016
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.