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Protein

Elongation factor Tu

Gene

tuf

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26GTPUniRule annotation8
Nucleotide bindingi83 – 87GTPUniRule annotation5
Nucleotide bindingi138 – 141GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

  • growth Source: MTBBASE
  • response to hypoxia Source: MTBBASE
  • response to iron ion Source: MTBBASE
  • translational elongation Source: GO_Central

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:Rv0685
ORF Names:MTCY210.02
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0685.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • plasma membrane Source: MTBBASE

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000913531 – 396Elongation factor TuAdd BLAST396

Proteomic databases

PaxDbiP9WNN1.
PRIDEiP9WNN1.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi83332.Rv0685.

Structurei

3D structure databases

ProteinModelPortaliP9WNN1.
SMRiP9WNN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 205tr-type GAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 26G1By similarity8
Regioni62 – 66G2By similarity5
Regioni83 – 86G3By similarity4
Regioni138 – 141G4By similarity4
Regioni175 – 177G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGV. Bacteria.
COG0050. LUCA.
KOiK02358.
OMAiYSTATRH.
PhylomeDBiP9WNN1.

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B. 1 hit.
InterProiView protein in InterPro
IPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
PfamiView protein in Pfam
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WNN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAKFQRTK PHVNIGTIGH VDHGKTTLTA AITKVLHDKF PDLNETKAFD
60 70 80 90 100
QIDNAPEERQ RGITINIAHV EYQTDKRHYA HVDAPGHADY IKNMITGAAQ
110 120 130 140 150
MDGAILVVAA TDGPMPQTRE HVLLARQVGV PYILVALNKA DAVDDEELLE
160 170 180 190 200
LVEMEVRELL AAQEFDEDAP VVRVSALKAL EGDAKWVASV EELMNAVDES
210 220 230 240 250
IPDPVRETDK PFLMPVEDVF TITGRGTVVT GRVERGVINV NEEVEIVGIR
260 270 280 290 300
PSTTKTTVTG VEMFRKLLDQ GQAGDNVGLL LRGVKREDVE RGQVVTKPGT
310 320 330 340 350
TTPHTEFEGQ VYILSKDEGG RHTPFFNNYR PQFYFRTTDV TGVVTLPEGT
360 370 380 390
EMVMPGDNTN ISVKLIQPVA MDEGLRFAIR EGGRTVGAGR VTKIIK
Length:396
Mass (Da):43,594
Last modified:April 16, 2014 - v1
Checksum:i85F9012F364692FF
GO

Sequence cautioni

The sequence CAA45101 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63539 Genomic DNA. Translation: CAA45102.1.
X63539 Genomic DNA. Translation: CAA45101.1. Different initiation.
AL123456 Genomic DNA. Translation: CCP43428.1.
PIRiA44795.
RefSeqiNP_215199.1. NC_000962.3.
WP_003403463.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP43428; CCP43428; Rv0685.
GeneIDi888262.
KEGGimtu:Rv0685.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63539 Genomic DNA. Translation: CAA45102.1.
X63539 Genomic DNA. Translation: CAA45101.1. Different initiation.
AL123456 Genomic DNA. Translation: CCP43428.1.
PIRiA44795.
RefSeqiNP_215199.1. NC_000962.3.
WP_003403463.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliP9WNN1.
SMRiP9WNN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv0685.

Proteomic databases

PaxDbiP9WNN1.
PRIDEiP9WNN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP43428; CCP43428; Rv0685.
GeneIDi888262.
KEGGimtu:Rv0685.

Organism-specific databases

TubercuListiRv0685.

Phylogenomic databases

eggNOGiENOG4105CGV. Bacteria.
COG0050. LUCA.
KOiK02358.
OMAiYSTATRH.
PhylomeDBiP9WNN1.

Family and domain databases

CDDicd03697. EFTU_II. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B. 1 hit.
InterProiView protein in InterPro
IPR004161. EFTu-like_2.
IPR033720. EFTU_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
PfamiView protein in Pfam
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEFTU_MYCTU
AccessioniPrimary (citable) accession number: P9WNN1
Secondary accession number(s): L0T782
, P0A558, P31501, P95031, Q50823
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 15, 2017
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.