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Protein

Elongation factor G

Gene

fusA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 27GTPBy similarity8
Nucleotide bindingi84 – 88GTPBy similarity5
Nucleotide bindingi138 – 141GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • growth Source: MTBBASE

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor G
Short name:
EF-G
Gene namesi
Name:fusA
Ordered Locus Names:Rv0684
ORF Names:MTCY210.01, MTV040.12
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0684

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: MTBBASE

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000911651 – 701Elongation factor GAdd BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki307Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP9WNM7
PRIDEiP9WNM7

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0684

Structurei

3D structure databases

ProteinModelPortaliP9WNM7
SMRiP9WNM7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 287tr-type GAdd BLAST277

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0480 LUCA
KOiK02355
OMAiISRIYQM
PhylomeDBiP9WNM7

Family and domain databases

CDDicd16262 EFG_III, 1 hit
cd01434 EFG_mtEFG1_IV, 1 hit
cd03713 EFG_mtEFG_C, 1 hit
Gene3Di3.30.230.10, 2 hits
HAMAPiMF_00054_B EF_G_EF_2_B, 1 hit
InterProiView protein in InterPro
IPR009022 EFG_III
IPR035647 EFG_III/V
IPR035649 EFG_V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR004540 Transl_elong_EFG/EF2
IPR005517 Transl_elong_EFG/EF2_IV
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF03764 EFG_IV, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SM00889 EFG_IV, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54211 SSF54211, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR00484 EF-G, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P9WNM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKDVLTDL SRVRNFGIMA HIDAGKTTTT ERILYYTGIN YKIGEVHDGA
60 70 80 90 100
ATMDWMEQEQ ERGITITSAA TTTFWKDNQL NIIDTPGHVD FTVEVERNLR
110 120 130 140 150
VLDGAVAVFD GKEGVEPQSE QVWRQADKYD VPRICFVNKM DKIGADFYFS
160 170 180 190 200
VRTMGERLGA NAVPIQLPVG AEADFEGVVD LVEMNAKVWR GETKLGETYD
210 220 230 240 250
TVEIPADLAE QAEEYRTKLL EVVAESDEHL LEKYLGGEEL TVDEIKGAIR
260 270 280 290 300
KLTIASEIYP VLCGSAFKNK GVQPMLDAVV DYLPSPLDVP PAIGHAPAKE
310 320 330 340 350
DEEVVRKATT DEPFAALAFK IATHPFFGKL TYIRVYSGTV ESGSQVINAT
360 370 380 390 400
KGKKERLGKL FQMHSNKENP VDRASAGHIY AVIGLKDTTT GDTLSDPNQQ
410 420 430 440 450
IVLESMTFPD PVIEVAIEPK TKSDQEKLSL SIQKLAEEDP TFKVHLDSET
460 470 480 490 500
GQTVIGGMGE LHLDILVDRM RREFKVEANV GKPQVAYKET IKRLVQNVEY
510 520 530 540 550
THKKQTGGSG QFAKVIINLE PFTGEEGATY EFESKVTGGR IPREYIPSVD
560 570 580 590 600
AGAQDAMQYG VLAGYPLVNL KVTLLDGAYH EVDSSEMAFK IAGSQVLKKA
610 620 630 640 650
AALAQPVILE PIMAVEVTTP EDYMGDVIGD LNSRRGQIQA MEERAGARVV
660 670 680 690 700
RAHVPLSEMF GYVGDLRSKT QGRANYSMVF DSYSEVPANV SKEIIAKATG

E
Length:701
Mass (Da):77,203
Last modified:April 16, 2014 - v1
Checksum:i0C6DD4C128E31EA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP43427.1
PIRiE70827
RefSeqiWP_003898554.1, NZ_KK339370.1
YP_177746.1, NC_000962.3

Genome annotation databases

EnsemblBacteriaiCCP43427; CCP43427; Rv0684
GeneIDi888240
KEGGimtu:Rv0684

Similar proteinsi

Entry informationi

Entry nameiEFG_MYCTU
AccessioniPrimary (citable) accession number: P9WNM7
Secondary accession number(s): L0T7E8, O53790, P0A556
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: May 23, 2018
This is version 29 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health