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Protein

Elongation factor G

Gene

fusA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 278GTPBy similarity
Nucleotide bindingi84 – 885GTPBy similarity
Nucleotide bindingi138 – 1414GTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • growth Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor G
Short name:
EF-G
Gene namesi
Name:fusA
Ordered Locus Names:Rv0684
ORF Names:MTCY210.01, MTV040.12
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0684.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytoplasm Source: UniProtKB-SubCell
  • intracellular Source: GO_Central
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 701701Elongation factor GPRO_0000091165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki307 – 307Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP9WNM7.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0684.

Structurei

3D structure databases

ProteinModelPortaliP9WNM7.
SMRiP9WNM7. Positions 10-700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 287277tr-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0480. LUCA.
KOiK02355.
OMAiKLGVAIQ.
PhylomeDBiP9WNM7.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WNM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKDVLTDL SRVRNFGIMA HIDAGKTTTT ERILYYTGIN YKIGEVHDGA
60 70 80 90 100
ATMDWMEQEQ ERGITITSAA TTTFWKDNQL NIIDTPGHVD FTVEVERNLR
110 120 130 140 150
VLDGAVAVFD GKEGVEPQSE QVWRQADKYD VPRICFVNKM DKIGADFYFS
160 170 180 190 200
VRTMGERLGA NAVPIQLPVG AEADFEGVVD LVEMNAKVWR GETKLGETYD
210 220 230 240 250
TVEIPADLAE QAEEYRTKLL EVVAESDEHL LEKYLGGEEL TVDEIKGAIR
260 270 280 290 300
KLTIASEIYP VLCGSAFKNK GVQPMLDAVV DYLPSPLDVP PAIGHAPAKE
310 320 330 340 350
DEEVVRKATT DEPFAALAFK IATHPFFGKL TYIRVYSGTV ESGSQVINAT
360 370 380 390 400
KGKKERLGKL FQMHSNKENP VDRASAGHIY AVIGLKDTTT GDTLSDPNQQ
410 420 430 440 450
IVLESMTFPD PVIEVAIEPK TKSDQEKLSL SIQKLAEEDP TFKVHLDSET
460 470 480 490 500
GQTVIGGMGE LHLDILVDRM RREFKVEANV GKPQVAYKET IKRLVQNVEY
510 520 530 540 550
THKKQTGGSG QFAKVIINLE PFTGEEGATY EFESKVTGGR IPREYIPSVD
560 570 580 590 600
AGAQDAMQYG VLAGYPLVNL KVTLLDGAYH EVDSSEMAFK IAGSQVLKKA
610 620 630 640 650
AALAQPVILE PIMAVEVTTP EDYMGDVIGD LNSRRGQIQA MEERAGARVV
660 670 680 690 700
RAHVPLSEMF GYVGDLRSKT QGRANYSMVF DSYSEVPANV SKEIIAKATG

E
Length:701
Mass (Da):77,203
Last modified:April 16, 2014 - v1
Checksum:i0C6DD4C128E31EA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43427.1.
PIRiE70827.
RefSeqiWP_003898554.1. NC_000962.3.
YP_177746.1. NC_000962.3.

Genome annotation databases

EnsemblBacteriaiCCP43427; CCP43427; Rv0684.
GeneIDi888240.
KEGGimtu:Rv0684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43427.1.
PIRiE70827.
RefSeqiWP_003898554.1. NC_000962.3.
YP_177746.1. NC_000962.3.

3D structure databases

ProteinModelPortaliP9WNM7.
SMRiP9WNM7. Positions 10-700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv0684.

Proteomic databases

PaxDbiP9WNM7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP43427; CCP43427; Rv0684.
GeneIDi888240.
KEGGimtu:Rv0684.

Organism-specific databases

TubercuListiRv0684.

Phylogenomic databases

eggNOGiCOG0480. LUCA.
KOiK02355.
OMAiKLGVAIQ.
PhylomeDBiP9WNM7.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFG_MYCTU
AccessioniPrimary (citable) accession number: P9WNM7
Secondary accession number(s): L0T7E8, O53790, P0A556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: September 7, 2016
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.