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P9WNL7

- EMBB_MYCTU

UniProt

P9WNL7 - EMBB_MYCTU

Protein

Probable arabinosyltransferase B

Gene

embB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 6 (01 Oct 2014)
      Sequence version 1 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.

    GO - Molecular functioni

    1. arabinosyltransferase activity Source: InterPro

    GO - Biological processi

    1. Actinobacterium-type cell wall biogenesis Source: InterPro
    2. growth Source: MTBBASE
    3. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Antibiotic resistance, Cell wall biogenesis/degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable arabinosyltransferase B (EC:2.4.2.-)
    Gene namesi
    Name:embB
    Ordered Locus Names:Rv3795
    ORF Names:MTCY13D12.29
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001584: Chromosome

    Organism-specific databases

    TubercuListiRv3795.

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall Source: MTBBASE
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: MTBBASE

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10981098Probable arabinosyltransferase BPRO_0000220569Add
    BLAST

    Expressioni

    Inductioni

    Positively regulated by the transcriptional regulatory protein EmbR.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP9WNL7.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei28 – 5023HelicalSequence AnalysisAdd
    BLAST
    Transmembranei217 – 23923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei271 – 29323HelicalSequence AnalysisAdd
    BLAST
    Transmembranei402 – 41918HelicalSequence AnalysisAdd
    BLAST
    Transmembranei434 – 45623HelicalSequence AnalysisAdd
    BLAST
    Transmembranei472 – 49423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei541 – 55818HelicalSequence AnalysisAdd
    BLAST
    Transmembranei570 – 58718HelicalSequence AnalysisAdd
    BLAST
    Transmembranei597 – 61923HelicalSequence AnalysisAdd
    BLAST
    Transmembranei626 – 64823HelicalSequence AnalysisAdd
    BLAST
    Transmembranei663 – 68523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei698 – 72023HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the emb family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK11386.
    PhylomeDBiP9WNL7.

    Family and domain databases

    Gene3Di2.60.120.610. 1 hit.
    InterProiIPR027451. EmbABC_N.
    IPR007680. Mycobac_arabinan_synth.
    [Graphical view]
    PfamiPF04602. Arabinose_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P9WNL7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQCASRRKS TPNRAILGAF ASARGTRWVA TIAGLIGFVL SVATPLLPVV     50
    QTTAMLDWPQ RGQLGSVTAP LISLTPVDFT ATVPCDVVRA MPPAGGVVLG 100
    TAPKQGKDAN LQALFVVVSA QRVDVTDRNV VILSVPREQV TSPQCQRIEV 150
    TSTHAGTFAN FVGLKDPSGA PLRSGFPDPN LRPQIVGVFT DLTGPAPPGL 200
    AVSATIDTRF STRPTTLKLL AIIGAIVATV VALIALWRLD QLDGRGSIAQ 250
    LLLRPFRPAS SPGGMRRLIP ASWRTFTLTD AVVIFGFLLW HVIGANSSDD 300
    GYILGMARVA DHAGYMSNYF RWFGSPEDPF GWYYNLLALM THVSDASLWM 350
    RLPDLAAGLV CWLLLSREVL PRLGPAVEAS KPAYWAAAMV LLTAWMPFNN 400
    GLRPEGIIAL GSLVTYVLIE RSMRYSRLTP AALAVVTAAF TLGVQPTGLI 450
    AVAALVAGGR PMLRILVRRH RLVGTLPLVS PMLAAGTVIL TVVFADQTLS 500
    TVLEATRVRA KIGPSQAWYT ENLRYYYLIL PTVDGSLSRR FGFLITALCL 550
    FTAVFIMLRR KRIPSVARGP AWRLMGVIFG TMFFLMFTPT KWVHHFGLFA 600
    AVGAAMAALT TVLVSPSVLR WSRNRMAFLA ALFFLLALCW ATTNGWWYVS 650
    SYGVPFNSAM PKIDGITVST IFFALFAIAA GYAAWLHFAP RGAGEGRLIR 700
    ALTTAPVPIV AGFMAAVFVA SMVAGIVRQY PTYSNGWSNV RAFVGGCGLA 750
    DDVLVEPDTN AGFMKPLDGD SGSWGPLGPL GGVNPVGFTP NGVPEHTVAE 800
    AIVMKPNQPG TDYDWDAPTK LTSPGINGST VPLPYGLDPA RVPLAGTYTT 850
    GAQQQSTLVS AWYLLPKPDD GHPLVVVTAA GKIAGNSVLH GYTPGQTVVL 900
    EYAMPGPGAL VPAGRMVPDD LYGEQPKAWR NLRFARAKMP ADAVAVRVVA 950
    EDLSLTPEDW IAVTPPRVPD LRSLQEYVGS TQPVLLDWAV GLAFPCQQPM 1000
    LHANGIAEIP KFRITPDYSA KKLDTDTWED GTNGGLLGIT DLLLRAHVMA 1050
    TYLSRDWARD WGSLRKFDTL VDAPPAQLEL GTATRSGLWS PGKIRIGP 1098
    Length:1,098
    Mass (Da):118,021
    Last modified:April 16, 2014 - v1
    Checksum:iDD7D7025DC803833
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti773 – 7742SW → FL(PubMed:9142129)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti297 – 2971S → A Resistance to EMB.
    Natural varianti306 – 3061M → I Resistance to EMB. 2 Publications
    Natural varianti306 – 3061M → L Resistance to EMB. 2 Publications
    Natural varianti306 – 3061M → V Resistance to EMB. 2 Publications
    Natural varianti328 – 3281D → G Resistance to EMB.
    Natural varianti328 – 3281D → Y Resistance to EMB.
    Natural varianti330 – 3301F → V Resistance to EMB. 1 Publication
    Natural varianti334 – 3341Y → H Resistance to EMB.
    Natural varianti406 – 4061G → A Resistance to EMB.
    Natural varianti406 – 4061G → C Resistance to EMB.
    Natural varianti406 – 4061G → D Resistance to EMB.
    Natural varianti497 – 4971Q → K Resistance to EMB.
    Natural varianti497 – 4971Q → R Resistance to EMB.
    Natural varianti745 – 7451G → D Resistance to EMB.
    Natural varianti959 – 9591D → A Resistance to EMB.
    Natural varianti1000 – 10001M → R Resistance to EMB.
    Natural varianti1024 – 10241D → N Resistance to EMB.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U68480 Genomic DNA. Translation: AAC45281.1.
    AL123456 Genomic DNA. Translation: CCP46624.1.
    PIRiG70697.
    RefSeqiNP_218312.1. NC_000962.3.
    YP_006517292.1. NC_018143.2.

    Genome annotation databases

    GeneIDi13317419.
    886126.
    KEGGimtu:Rv3795.
    mtv:RVBD_3795.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U68480 Genomic DNA. Translation: AAC45281.1 .
    AL123456 Genomic DNA. Translation: CCP46624.1 .
    PIRi G70697.
    RefSeqi NP_218312.1. NC_000962.3.
    YP_006517292.1. NC_018143.2.

    3D structure databases

    ProteinModelPortali P9WNL7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13317419.
    886126.
    KEGGi mtu:Rv3795.
    mtv:RVBD_3795.

    Organism-specific databases

    TubercuListi Rv3795.

    Phylogenomic databases

    KOi K11386.
    PhylomeDBi P9WNL7.

    Family and domain databases

    Gene3Di 2.60.120.610. 1 hit.
    InterProi IPR027451. EmbABC_N.
    IPR007680. Mycobac_arabinan_synth.
    [Graphical view ]
    Pfami PF04602. Arabinose_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol."
      Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr.
      Nat. Med. 3:567-570(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 25618 / H37Rv.
    4. "Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations."
      Sreevatsan S., Stockbauer K.E., Pan X., Kreiswirth B.N., Moghazeh S.L., Jacobs W.R. Jr., Telenti A., Musser J.M.
      Antimicrob. Agents Chemother. 41:1677-1681(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306; VAL-306 AND VAL-330.
    5. "Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis."
      Ramaswamy S.V., Amin A.G., Goeksel S., Stager C.E., Dou S.-J., El Sahly H., Moghazeh S.L., Kreiswirth B.N., Musser J.M.
      Antimicrob. Agents Chemother. 44:326-336(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS EMB RESISTANT.
    6. "Detection of embB codon 306 mutations in ethambutol resistant Mycobacterium tuberculosis directly from sputum samples: a low-cost, rapid approach."
      Rinder H., Mieskes K.T., Tortoli E., Richter E., Casal M., Vaquero M., Cambau E., Feldmann K., Loescher T.
      Mol. Cell. Probes 15:37-42(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306 AND VAL-306.
    7. "Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo."
      Sharma K., Gupta M., Pathak M., Gupta N., Koul A., Sarangi S., Baweja R., Singh Y.
      J. Bacteriol. 188:2936-2944(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiEMBB_MYCTU
    AccessioniPrimary (citable) accession number: P9WNL7
    Secondary accession number(s): L0TDT8, P72030, P72061
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 6 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3