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P9WNL7

- EMBB_MYCTU

UniProt

P9WNL7 - EMBB_MYCTU

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Protein

Probable arabinosyltransferase B

Gene

embB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.

GO - Molecular functioni

  1. arabinosyltransferase activity Source: InterPro

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
  3. growth Source: MTBBASE
  4. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable arabinosyltransferase B (EC:2.4.2.-)
Gene namesi
Name:embB
Ordered Locus Names:Rv3795
ORF Names:MTCY13D12.29
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv3795.

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: MTBBASE
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10981098Probable arabinosyltransferase BPRO_0000220569Add
BLAST

Expressioni

Inductioni

Positively regulated by the transcriptional regulatory protein EmbR.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP9WNL7.
SMRiP9WNL7. Positions 56-200, 734-1067.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei28 – 5023HelicalSequence AnalysisAdd
BLAST
Transmembranei217 – 23923HelicalSequence AnalysisAdd
BLAST
Transmembranei271 – 29323HelicalSequence AnalysisAdd
BLAST
Transmembranei402 – 41918HelicalSequence AnalysisAdd
BLAST
Transmembranei434 – 45623HelicalSequence AnalysisAdd
BLAST
Transmembranei472 – 49423HelicalSequence AnalysisAdd
BLAST
Transmembranei541 – 55818HelicalSequence AnalysisAdd
BLAST
Transmembranei570 – 58718HelicalSequence AnalysisAdd
BLAST
Transmembranei597 – 61923HelicalSequence AnalysisAdd
BLAST
Transmembranei626 – 64823HelicalSequence AnalysisAdd
BLAST
Transmembranei663 – 68523HelicalSequence AnalysisAdd
BLAST
Transmembranei698 – 72023HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the emb family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK11386.
PhylomeDBiP9WNL7.

Family and domain databases

Gene3Di2.60.120.610. 1 hit.
InterProiIPR027451. EmbABC_N.
IPR007680. Mycobac_arabinan_synth.
[Graphical view]
PfamiPF04602. Arabinose_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WNL7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQCASRRKS TPNRAILGAF ASARGTRWVA TIAGLIGFVL SVATPLLPVV
60 70 80 90 100
QTTAMLDWPQ RGQLGSVTAP LISLTPVDFT ATVPCDVVRA MPPAGGVVLG
110 120 130 140 150
TAPKQGKDAN LQALFVVVSA QRVDVTDRNV VILSVPREQV TSPQCQRIEV
160 170 180 190 200
TSTHAGTFAN FVGLKDPSGA PLRSGFPDPN LRPQIVGVFT DLTGPAPPGL
210 220 230 240 250
AVSATIDTRF STRPTTLKLL AIIGAIVATV VALIALWRLD QLDGRGSIAQ
260 270 280 290 300
LLLRPFRPAS SPGGMRRLIP ASWRTFTLTD AVVIFGFLLW HVIGANSSDD
310 320 330 340 350
GYILGMARVA DHAGYMSNYF RWFGSPEDPF GWYYNLLALM THVSDASLWM
360 370 380 390 400
RLPDLAAGLV CWLLLSREVL PRLGPAVEAS KPAYWAAAMV LLTAWMPFNN
410 420 430 440 450
GLRPEGIIAL GSLVTYVLIE RSMRYSRLTP AALAVVTAAF TLGVQPTGLI
460 470 480 490 500
AVAALVAGGR PMLRILVRRH RLVGTLPLVS PMLAAGTVIL TVVFADQTLS
510 520 530 540 550
TVLEATRVRA KIGPSQAWYT ENLRYYYLIL PTVDGSLSRR FGFLITALCL
560 570 580 590 600
FTAVFIMLRR KRIPSVARGP AWRLMGVIFG TMFFLMFTPT KWVHHFGLFA
610 620 630 640 650
AVGAAMAALT TVLVSPSVLR WSRNRMAFLA ALFFLLALCW ATTNGWWYVS
660 670 680 690 700
SYGVPFNSAM PKIDGITVST IFFALFAIAA GYAAWLHFAP RGAGEGRLIR
710 720 730 740 750
ALTTAPVPIV AGFMAAVFVA SMVAGIVRQY PTYSNGWSNV RAFVGGCGLA
760 770 780 790 800
DDVLVEPDTN AGFMKPLDGD SGSWGPLGPL GGVNPVGFTP NGVPEHTVAE
810 820 830 840 850
AIVMKPNQPG TDYDWDAPTK LTSPGINGST VPLPYGLDPA RVPLAGTYTT
860 870 880 890 900
GAQQQSTLVS AWYLLPKPDD GHPLVVVTAA GKIAGNSVLH GYTPGQTVVL
910 920 930 940 950
EYAMPGPGAL VPAGRMVPDD LYGEQPKAWR NLRFARAKMP ADAVAVRVVA
960 970 980 990 1000
EDLSLTPEDW IAVTPPRVPD LRSLQEYVGS TQPVLLDWAV GLAFPCQQPM
1010 1020 1030 1040 1050
LHANGIAEIP KFRITPDYSA KKLDTDTWED GTNGGLLGIT DLLLRAHVMA
1060 1070 1080 1090
TYLSRDWARD WGSLRKFDTL VDAPPAQLEL GTATRSGLWS PGKIRIGP
Length:1,098
Mass (Da):118,021
Last modified:April 16, 2014 - v1
Checksum:iDD7D7025DC803833
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti773 – 7742SW → FL(PubMed:9142129)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti297 – 2971S → A Resistance to EMB.
Natural varianti306 – 3061M → I Resistance to EMB. 2 Publications
Natural varianti306 – 3061M → L Resistance to EMB. 2 Publications
Natural varianti306 – 3061M → V Resistance to EMB. 2 Publications
Natural varianti328 – 3281D → G Resistance to EMB.
Natural varianti328 – 3281D → Y Resistance to EMB.
Natural varianti330 – 3301F → V Resistance to EMB. 1 Publication
Natural varianti334 – 3341Y → H Resistance to EMB.
Natural varianti406 – 4061G → A Resistance to EMB.
Natural varianti406 – 4061G → C Resistance to EMB.
Natural varianti406 – 4061G → D Resistance to EMB.
Natural varianti497 – 4971Q → K Resistance to EMB.
Natural varianti497 – 4971Q → R Resistance to EMB.
Natural varianti745 – 7451G → D Resistance to EMB.
Natural varianti959 – 9591D → A Resistance to EMB.
Natural varianti1000 – 10001M → R Resistance to EMB.
Natural varianti1024 – 10241D → N Resistance to EMB.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U68480 Genomic DNA. Translation: AAC45281.1.
AL123456 Genomic DNA. Translation: CCP46624.1.
PIRiG70697.
RefSeqiNP_218312.1. NC_000962.3.
YP_006517292.1. NC_018143.2.

Genome annotation databases

GeneIDi13317419.
886126.
KEGGimtu:Rv3795.
mtv:RVBD_3795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U68480 Genomic DNA. Translation: AAC45281.1 .
AL123456 Genomic DNA. Translation: CCP46624.1 .
PIRi G70697.
RefSeqi NP_218312.1. NC_000962.3.
YP_006517292.1. NC_018143.2.

3D structure databases

ProteinModelPortali P9WNL7.
SMRi P9WNL7. Positions 56-200, 734-1067.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13317419.
886126.
KEGGi mtu:Rv3795.
mtv:RVBD_3795.

Organism-specific databases

TubercuListi Rv3795.

Phylogenomic databases

KOi K11386.
PhylomeDBi P9WNL7.

Family and domain databases

Gene3Di 2.60.120.610. 1 hit.
InterProi IPR027451. EmbABC_N.
IPR007680. Mycobac_arabinan_synth.
[Graphical view ]
Pfami PF04602. Arabinose_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol."
    Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr.
    Nat. Med. 3:567-570(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  4. "Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations."
    Sreevatsan S., Stockbauer K.E., Pan X., Kreiswirth B.N., Moghazeh S.L., Jacobs W.R. Jr., Telenti A., Musser J.M.
    Antimicrob. Agents Chemother. 41:1677-1681(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306; VAL-306 AND VAL-330.
  5. "Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis."
    Ramaswamy S.V., Amin A.G., Goeksel S., Stager C.E., Dou S.-J., El Sahly H., Moghazeh S.L., Kreiswirth B.N., Musser J.M.
    Antimicrob. Agents Chemother. 44:326-336(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS EMB RESISTANT.
  6. "Detection of embB codon 306 mutations in ethambutol resistant Mycobacterium tuberculosis directly from sputum samples: a low-cost, rapid approach."
    Rinder H., Mieskes K.T., Tortoli E., Richter E., Casal M., Vaquero M., Cambau E., Feldmann K., Loescher T.
    Mol. Cell. Probes 15:37-42(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306 AND VAL-306.
  7. "Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo."
    Sharma K., Gupta M., Pathak M., Gupta N., Koul A., Sarangi S., Baweja R., Singh Y.
    J. Bacteriol. 188:2936-2944(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiEMBB_MYCTU
AccessioniPrimary (citable) accession number: P9WNL7
Secondary accession number(s): L0TDT8, P72030, P72061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 29, 2014
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3