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P9WNL7

- EMBB_MYCTU

UniProt

P9WNL7 - EMBB_MYCTU

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Protein

Probable arabinosyltransferase B

Gene
embB, Rv3795, MTCY13D12.29
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.

GO - Molecular functioni

  1. arabinosyltransferase activity Source: InterPro

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable arabinosyltransferase B (EC:2.4.2.-)
Gene namesi
Name:embB
Ordered Locus Names:Rv3795
ORF Names:MTCY13D12.29
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv3795.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei28 – 5023Helical; Reviewed predictionAdd
BLAST
Transmembranei217 – 23923Helical; Reviewed predictionAdd
BLAST
Transmembranei271 – 29323Helical; Reviewed predictionAdd
BLAST
Transmembranei402 – 41918Helical; Reviewed predictionAdd
BLAST
Transmembranei434 – 45623Helical; Reviewed predictionAdd
BLAST
Transmembranei472 – 49423Helical; Reviewed predictionAdd
BLAST
Transmembranei541 – 55818Helical; Reviewed predictionAdd
BLAST
Transmembranei570 – 58718Helical; Reviewed predictionAdd
BLAST
Transmembranei597 – 61923Helical; Reviewed predictionAdd
BLAST
Transmembranei626 – 64823Helical; Reviewed predictionAdd
BLAST
Transmembranei663 – 68523Helical; Reviewed predictionAdd
BLAST
Transmembranei698 – 72023Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10981098Probable arabinosyltransferase BPRO_0000220569Add
BLAST

Expressioni

Inductioni

Positively regulated by the transcriptional regulatory protein EmbR.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP9WNL7.

Family & Domainsi

Sequence similaritiesi

Belongs to the emb family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK11386.
PhylomeDBiP9WNL7.

Family and domain databases

Gene3Di2.60.120.610. 1 hit.
InterProiIPR027451. EmbABC_N.
IPR007680. Mycobac_arabinan_synth.
[Graphical view]
PfamiPF04602. Arabinose_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WNL7-1 [UniParc]FASTAAdd to Basket

« Hide

MTQCASRRKS TPNRAILGAF ASARGTRWVA TIAGLIGFVL SVATPLLPVV     50
QTTAMLDWPQ RGQLGSVTAP LISLTPVDFT ATVPCDVVRA MPPAGGVVLG 100
TAPKQGKDAN LQALFVVVSA QRVDVTDRNV VILSVPREQV TSPQCQRIEV 150
TSTHAGTFAN FVGLKDPSGA PLRSGFPDPN LRPQIVGVFT DLTGPAPPGL 200
AVSATIDTRF STRPTTLKLL AIIGAIVATV VALIALWRLD QLDGRGSIAQ 250
LLLRPFRPAS SPGGMRRLIP ASWRTFTLTD AVVIFGFLLW HVIGANSSDD 300
GYILGMARVA DHAGYMSNYF RWFGSPEDPF GWYYNLLALM THVSDASLWM 350
RLPDLAAGLV CWLLLSREVL PRLGPAVEAS KPAYWAAAMV LLTAWMPFNN 400
GLRPEGIIAL GSLVTYVLIE RSMRYSRLTP AALAVVTAAF TLGVQPTGLI 450
AVAALVAGGR PMLRILVRRH RLVGTLPLVS PMLAAGTVIL TVVFADQTLS 500
TVLEATRVRA KIGPSQAWYT ENLRYYYLIL PTVDGSLSRR FGFLITALCL 550
FTAVFIMLRR KRIPSVARGP AWRLMGVIFG TMFFLMFTPT KWVHHFGLFA 600
AVGAAMAALT TVLVSPSVLR WSRNRMAFLA ALFFLLALCW ATTNGWWYVS 650
SYGVPFNSAM PKIDGITVST IFFALFAIAA GYAAWLHFAP RGAGEGRLIR 700
ALTTAPVPIV AGFMAAVFVA SMVAGIVRQY PTYSNGWSNV RAFVGGCGLA 750
DDVLVEPDTN AGFMKPLDGD SGSWGPLGPL GGVNPVGFTP NGVPEHTVAE 800
AIVMKPNQPG TDYDWDAPTK LTSPGINGST VPLPYGLDPA RVPLAGTYTT 850
GAQQQSTLVS AWYLLPKPDD GHPLVVVTAA GKIAGNSVLH GYTPGQTVVL 900
EYAMPGPGAL VPAGRMVPDD LYGEQPKAWR NLRFARAKMP ADAVAVRVVA 950
EDLSLTPEDW IAVTPPRVPD LRSLQEYVGS TQPVLLDWAV GLAFPCQQPM 1000
LHANGIAEIP KFRITPDYSA KKLDTDTWED GTNGGLLGIT DLLLRAHVMA 1050
TYLSRDWARD WGSLRKFDTL VDAPPAQLEL GTATRSGLWS PGKIRIGP 1098
Length:1,098
Mass (Da):118,021
Last modified:April 16, 2014 - v1
Checksum:iDD7D7025DC803833
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti297 – 2971S → A Resistance to EMB.
Natural varianti306 – 3061M → I Resistance to EMB. 2 Publications
Natural varianti306 – 3061M → L Resistance to EMB. 2 Publications
Natural varianti306 – 3061M → V Resistance to EMB. 2 Publications
Natural varianti328 – 3281D → G Resistance to EMB.
Natural varianti328 – 3281D → Y Resistance to EMB.
Natural varianti330 – 3301F → V Resistance to EMB. 1 Publication
Natural varianti334 – 3341Y → H Resistance to EMB.
Natural varianti406 – 4061G → A Resistance to EMB.
Natural varianti406 – 4061G → C Resistance to EMB.
Natural varianti406 – 4061G → D Resistance to EMB.
Natural varianti497 – 4971Q → K Resistance to EMB.
Natural varianti497 – 4971Q → R Resistance to EMB.
Natural varianti745 – 7451G → D Resistance to EMB.
Natural varianti959 – 9591D → A Resistance to EMB.
Natural varianti1000 – 10001M → R Resistance to EMB.
Natural varianti1024 – 10241D → N Resistance to EMB.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti773 – 7742SW → FL1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U68480 Genomic DNA. Translation: AAC45281.1.
AL123456 Genomic DNA. Translation: CCP46624.1.
PIRiG70697.
RefSeqiNP_218312.1. NC_000962.3.
YP_006517292.1. NC_018143.2.

Genome annotation databases

GeneIDi13317419.
886126.
KEGGimtu:Rv3795.
mtv:RVBD_3795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U68480 Genomic DNA. Translation: AAC45281.1 .
AL123456 Genomic DNA. Translation: CCP46624.1 .
PIRi G70697.
RefSeqi NP_218312.1. NC_000962.3.
YP_006517292.1. NC_018143.2.

3D structure databases

ProteinModelPortali P9WNL7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13317419.
886126.
KEGGi mtu:Rv3795.
mtv:RVBD_3795.

Organism-specific databases

TubercuListi Rv3795.

Phylogenomic databases

KOi K11386.
PhylomeDBi P9WNL7.

Family and domain databases

Gene3Di 2.60.120.610. 1 hit.
InterProi IPR027451. EmbABC_N.
IPR007680. Mycobac_arabinan_synth.
[Graphical view ]
Pfami PF04602. Arabinose_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol."
    Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr.
    Nat. Med. 3:567-570(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  4. "Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations."
    Sreevatsan S., Stockbauer K.E., Pan X., Kreiswirth B.N., Moghazeh S.L., Jacobs W.R. Jr., Telenti A., Musser J.M.
    Antimicrob. Agents Chemother. 41:1677-1681(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306; VAL-306 AND VAL-330.
  5. "Molecular genetic analysis of nucleotide polymorphisms associated with ethambutol resistance in human isolates of Mycobacterium tuberculosis."
    Ramaswamy S.V., Amin A.G., Goeksel S., Stager C.E., Dou S.-J., El Sahly H., Moghazeh S.L., Kreiswirth B.N., Musser J.M.
    Antimicrob. Agents Chemother. 44:326-336(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS EMB RESISTANT.
  6. "Detection of embB codon 306 mutations in ethambutol resistant Mycobacterium tuberculosis directly from sputum samples: a low-cost, rapid approach."
    Rinder H., Mieskes K.T., Tortoli E., Richter E., Casal M., Vaquero M., Cambau E., Feldmann K., Loescher T.
    Mol. Cell. Probes 15:37-42(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS EMB RESISTANT LEU-306; ILE-306 AND VAL-306.
  7. "Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo."
    Sharma K., Gupta M., Pathak M., Gupta N., Koul A., Sarangi S., Baweja R., Singh Y.
    J. Bacteriol. 188:2936-2944(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiEMBB_MYCTU
AccessioniPrimary (citable) accession number: P9WNL7
Secondary accession number(s): L0TDT8, P72030, P72061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: September 3, 2014
This is version 5 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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