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Protein

Probable arabinosyltransferase B

Gene

embB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.

GO - Molecular functioni

GO - Biological processi

  • Actinobacterium-type cell wall biogenesis Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • growth Source: MTBBASE
  • response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-8091-MONOMER.

Protein family/group databases

CAZyiGT53. Glycosyltransferase Family 53.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable arabinosyltransferase B (EC:2.4.2.-)
Gene namesi
Name:embB
Ordered Locus Names:Rv3795
ORF Names:MTCY13D12.29
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3795.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei28 – 50HelicalSequence analysisAdd BLAST23
Transmembranei217 – 239HelicalSequence analysisAdd BLAST23
Transmembranei271 – 293HelicalSequence analysisAdd BLAST23
Transmembranei402 – 419HelicalSequence analysisAdd BLAST18
Transmembranei434 – 456HelicalSequence analysisAdd BLAST23
Transmembranei472 – 494HelicalSequence analysisAdd BLAST23
Transmembranei541 – 558HelicalSequence analysisAdd BLAST18
Transmembranei570 – 587HelicalSequence analysisAdd BLAST18
Transmembranei597 – 619HelicalSequence analysisAdd BLAST23
Transmembranei626 – 648HelicalSequence analysisAdd BLAST23
Transmembranei663 – 685HelicalSequence analysisAdd BLAST23
Transmembranei698 – 720HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002205691 – 1098Probable arabinosyltransferase BAdd BLAST1098

Proteomic databases

PaxDbiP9WNL7.

Expressioni

Inductioni

Positively regulated by the transcriptional regulatory protein EmbR.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv3795.

Structurei

3D structure databases

ProteinModelPortaliP9WNL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the emb family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105J1Z. Bacteria.
ENOG410XSQE. LUCA.
KOiK11386.
OMAiLRYYYLF.
PhylomeDBiP9WNL7.

Family and domain databases

Gene3Di2.60.120.610. 1 hit.
InterProiIPR032731. Arabino_trans_C.
IPR007680. Arabino_trans_N.
IPR027451. EmbABC_1.
[Graphical view]
PfamiPF14896. Arabino_trans_C. 1 hit.
PF04602. Arabinose_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WNL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQCASRRKS TPNRAILGAF ASARGTRWVA TIAGLIGFVL SVATPLLPVV
60 70 80 90 100
QTTAMLDWPQ RGQLGSVTAP LISLTPVDFT ATVPCDVVRA MPPAGGVVLG
110 120 130 140 150
TAPKQGKDAN LQALFVVVSA QRVDVTDRNV VILSVPREQV TSPQCQRIEV
160 170 180 190 200
TSTHAGTFAN FVGLKDPSGA PLRSGFPDPN LRPQIVGVFT DLTGPAPPGL
210 220 230 240 250
AVSATIDTRF STRPTTLKLL AIIGAIVATV VALIALWRLD QLDGRGSIAQ
260 270 280 290 300
LLLRPFRPAS SPGGMRRLIP ASWRTFTLTD AVVIFGFLLW HVIGANSSDD
310 320 330 340 350
GYILGMARVA DHAGYMSNYF RWFGSPEDPF GWYYNLLALM THVSDASLWM
360 370 380 390 400
RLPDLAAGLV CWLLLSREVL PRLGPAVEAS KPAYWAAAMV LLTAWMPFNN
410 420 430 440 450
GLRPEGIIAL GSLVTYVLIE RSMRYSRLTP AALAVVTAAF TLGVQPTGLI
460 470 480 490 500
AVAALVAGGR PMLRILVRRH RLVGTLPLVS PMLAAGTVIL TVVFADQTLS
510 520 530 540 550
TVLEATRVRA KIGPSQAWYT ENLRYYYLIL PTVDGSLSRR FGFLITALCL
560 570 580 590 600
FTAVFIMLRR KRIPSVARGP AWRLMGVIFG TMFFLMFTPT KWVHHFGLFA
610 620 630 640 650
AVGAAMAALT TVLVSPSVLR WSRNRMAFLA ALFFLLALCW ATTNGWWYVS
660 670 680 690 700
SYGVPFNSAM PKIDGITVST IFFALFAIAA GYAAWLHFAP RGAGEGRLIR
710 720 730 740 750
ALTTAPVPIV AGFMAAVFVA SMVAGIVRQY PTYSNGWSNV RAFVGGCGLA
760 770 780 790 800
DDVLVEPDTN AGFMKPLDGD SGSWGPLGPL GGVNPVGFTP NGVPEHTVAE
810 820 830 840 850
AIVMKPNQPG TDYDWDAPTK LTSPGINGST VPLPYGLDPA RVPLAGTYTT
860 870 880 890 900
GAQQQSTLVS AWYLLPKPDD GHPLVVVTAA GKIAGNSVLH GYTPGQTVVL
910 920 930 940 950
EYAMPGPGAL VPAGRMVPDD LYGEQPKAWR NLRFARAKMP ADAVAVRVVA
960 970 980 990 1000
EDLSLTPEDW IAVTPPRVPD LRSLQEYVGS TQPVLLDWAV GLAFPCQQPM
1010 1020 1030 1040 1050
LHANGIAEIP KFRITPDYSA KKLDTDTWED GTNGGLLGIT DLLLRAHVMA
1060 1070 1080 1090
TYLSRDWARD WGSLRKFDTL VDAPPAQLEL GTATRSGLWS PGKIRIGP
Length:1,098
Mass (Da):118,021
Last modified:April 16, 2014 - v1
Checksum:iDD7D7025DC803833
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti773 – 774SW → FL (PubMed:9142129).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti297S → A Resistance to EMB. 1
Natural varianti306M → I Resistance to EMB. 2 Publications1
Natural varianti306M → L Resistance to EMB. 2 Publications1
Natural varianti306M → V Resistance to EMB. 2 Publications1
Natural varianti328D → G Resistance to EMB. 1
Natural varianti328D → Y Resistance to EMB. 1
Natural varianti330F → V Resistance to EMB. 1 Publication1
Natural varianti334Y → H Resistance to EMB. 1
Natural varianti406G → A Resistance to EMB. 1
Natural varianti406G → C Resistance to EMB. 1
Natural varianti406G → D Resistance to EMB. 1
Natural varianti497Q → K Resistance to EMB. 1
Natural varianti497Q → R Resistance to EMB. 1
Natural varianti745G → D Resistance to EMB. 1
Natural varianti959D → A Resistance to EMB. 1
Natural varianti1000M → R Resistance to EMB. 1
Natural varianti1024D → N Resistance to EMB. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68480 Genomic DNA. Translation: AAC45281.1.
AL123456 Genomic DNA. Translation: CCP46624.1.
PIRiG70697.
RefSeqiNP_218312.1. NC_000962.3.
WP_003901728.1. NC_000962.3.

Genome annotation databases

EnsemblBacteriaiCCP46624; CCP46624; Rv3795.
GeneIDi886126.
KEGGimtu:Rv3795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68480 Genomic DNA. Translation: AAC45281.1.
AL123456 Genomic DNA. Translation: CCP46624.1.
PIRiG70697.
RefSeqiNP_218312.1. NC_000962.3.
WP_003901728.1. NC_000962.3.

3D structure databases

ProteinModelPortaliP9WNL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3795.

Protein family/group databases

CAZyiGT53. Glycosyltransferase Family 53.

Proteomic databases

PaxDbiP9WNL7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46624; CCP46624; Rv3795.
GeneIDi886126.
KEGGimtu:Rv3795.

Organism-specific databases

TubercuListiRv3795.

Phylogenomic databases

eggNOGiENOG4105J1Z. Bacteria.
ENOG410XSQE. LUCA.
KOiK11386.
OMAiLRYYYLF.
PhylomeDBiP9WNL7.

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-8091-MONOMER.

Family and domain databases

Gene3Di2.60.120.610. 1 hit.
InterProiIPR032731. Arabino_trans_C.
IPR007680. Arabino_trans_N.
IPR027451. EmbABC_1.
[Graphical view]
PfamiPF14896. Arabino_trans_C. 1 hit.
PF04602. Arabinose_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMBB_MYCTU
AccessioniPrimary (citable) accession number: P9WNL7
Secondary accession number(s): L0TDT8, P72030, P72061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.