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Protein

Ferritin BfrB

Gene

bfrB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe2+ ions into Fe3+ ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex.1 Publication

Catalytic activityi

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi22 – 221Iron 1PROSITE-ProRule annotation
Metal bindingi55 – 551Iron 1PROSITE-ProRule annotation
Metal bindingi55 – 551Iron 2PROSITE-ProRule annotation
Metal bindingi58 – 581Iron 1PROSITE-ProRule annotation
Metal bindingi99 – 991Iron 2PROSITE-ProRule annotation
Metal bindingi132 – 1321Iron 2PROSITE-ProRule annotation

GO - Molecular functioni

  • ferric iron binding Source: InterPro
  • ferroxidase activity Source: MTBBASE

GO - Biological processi

  • intracellular sequestering of iron ion Source: MTBBASE
  • iron assimilation by chelation and transport Source: Reactome
  • iron ion transport Source: InterPro
  • response to hypoxia Source: MTBBASE
  • response to nitrosative stress Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Iron storage

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-1222449. Mtb iron assimilation by chelation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferritin BfrB (EC:1.16.3.1)
Alternative name(s):
Non-heme ferritin Ftn
Nox19
Gene namesi
Name:bfrB
Synonyms:ftn
Ordered Locus Names:Rv3841
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3841.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular region Source: MTBBASE
  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 181180Ferritin BfrBPRO_0000392942Add
BLAST

Proteomic databases

PaxDbiP9WNE5.

Expressioni

Inductioni

Induced by 1 mM reactive nitrogen intermediate sodium nitroprusside dihydrate (at protein level). Induced by exposure to 50 µM FeCl3. A possible member of the dormancy regulon. Induced in response to reduced oxygen tension (hypoxia), repressed by carbon monoxide (CO). It is hoped that this regulon will give insight into the latent, or dormant phase of infection.4 Publications

Interactioni

Subunit structurei

Homooligomer of 24 subunits that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be stored.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv3841.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 3829Combined sources
Helixi42 – 6827Combined sources
Helixi88 – 11528Combined sources
Helixi119 – 14931Combined sources
Helixi153 – 16311Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QD8X-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-181[»]
3UNOX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-181[»]
ProteinModelPortaliP9WNE5.
SMRiP9WNE5. Positions 8-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 150146Ferritin-like diironPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ferritin family. Prokaryotic subfamily.Curated
Contains 1 ferritin-like diiron domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107YEV. Bacteria.
COG1528. LUCA.
OMAiNEFNASH.
PhylomeDBiP9WNE5.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR001519. Ferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PANTHERiPTHR11431. PTHR11431. 1 hit.
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WNE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYEGPKTK FHALMQEQIH NEFTAAQQYV AIAVYFDSED LPQLAKHFYS
60 70 80 90 100
QAVEERNHAM MLVQHLLDRD LRVEIPGVDT VRNQFDRPRE ALALALDQER
110 120 130 140 150
TVTDQVGRLT AVARDEGDFL GEQFMQWFLQ EQIEEVALMA TLVRVADRAG
160 170 180
ANLFELENFV AREVDVAPAA SGAPHAAGGR L
Length:181
Mass (Da):20,442
Last modified:April 16, 2014 - v1
Checksum:iC0F375669A292F55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102693 Genomic DNA. Translation: AAF06357.1.
AL123456 Genomic DNA. Translation: CCP46670.1.
PIRiF70653.
RefSeqiNP_218358.1. NC_000962.3.
WP_003420920.1. NZ_KK339374.1.

Genome annotation databases

EnsemblBacteriaiCCP46670; CCP46670; Rv3841.
GeneIDi886176.
KEGGimtu:Rv3841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102693 Genomic DNA. Translation: AAF06357.1.
AL123456 Genomic DNA. Translation: CCP46670.1.
PIRiF70653.
RefSeqiNP_218358.1. NC_000962.3.
WP_003420920.1. NZ_KK339374.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QD8X-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-181[»]
3UNOX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-181[»]
ProteinModelPortaliP9WNE5.
SMRiP9WNE5. Positions 8-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3841.

Proteomic databases

PaxDbiP9WNE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46670; CCP46670; Rv3841.
GeneIDi886176.
KEGGimtu:Rv3841.

Organism-specific databases

TubercuListiRv3841.

Phylogenomic databases

eggNOGiENOG4107YEV. Bacteria.
COG1528. LUCA.
OMAiNEFNASH.
PhylomeDBiP9WNE5.

Enzyme and pathway databases

ReactomeiR-HSA-1222449. Mtb iron assimilation by chelation.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR001519. Ferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PANTHERiPTHR11431. PTHR11431. 1 hit.
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "Response to reactive nitrogen intermediates in Mycobacterium tuberculosis: induction of the 16-kilodalton alpha-crystallin homolog by exposure to nitric oxide donors."
    Garbe T.R., Hibler N.S., Deretic V.
    Infect. Immun. 67:460-465(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-17, INDUCTION BY NITRIC OXIDE STRESS.
    Strain: ATCC 25618 / H37Rv.
  3. "Regulation of the Mycobacterium tuberculosis hypoxic response gene encoding alpha -crystallin."
    Sherman D.R., Voskuil M., Schnappinger D., Liao R., Harrell M.I., Schoolnik G.K.
    Proc. Natl. Acad. Sci. U.S.A. 98:7534-7539(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HYPOXIA.
    Strain: ATCC 25618 / H37Rv.
  4. "Hypoxic response of Mycobacterium tuberculosis studied by metabolic labeling and proteome analysis of cellular and extracellular proteins."
    Rosenkrands I., Slayden R.A., Crawford J., Aagaard C., Barry C.E. III, Andersen P.
    J. Bacteriol. 184:3485-3491(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION BY HYPOXIA.
    Strain: ATCC 25618 / H37Rv.
  5. "IdeR, an essential gene in Mycobacterium tuberculosis: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response."
    Rodriguez G.M., Voskuil M.I., Gold B., Schoolnik G.K., Smith I.
    Infect. Immun. 70:3371-3381(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCED BY IRON.
    Strain: ATCC 25618 / H37Rv.
  6. "Mycobacterium tuberculosis senses host-derived carbon monoxide during macrophage infection."
    Shiloh M.U., Manzanillo P., Cox J.S.
    Cell Host Microbe 3:323-330(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPRESSION BY CARBON MONOXIDE (CO).
    Strain: ATCC 35801 / TMC 107 / Erdman.
  7. "Crystallization and preliminary X-ray crystallographic analysis of a Mycobacterium tuberculosis ferritin homolog, BfrB."
    McMath L.M., Habel J.E., Sankaran B., Yu M., Hung L.W., Goulding C.W.
    Acta Crystallogr. F 66:1657-1661(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Strain: ATCC 25618 / H37Rv.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  9. "Ferritin structure from Mycobacterium tuberculosis: comparative study with homologues identifies extended C-terminus involved in ferroxidase activity."
    Khare G., Gupta V., Nangpal P., Gupta R.K., Sauter N.K., Tyagi A.K.
    PLoS ONE 6:E18570-E18570(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiBFRB_MYCTU
AccessioniPrimary (citable) accession number: P9WNE5
Secondary accession number(s): L0TGW6
, P96237, Q7BS08, Q7D4R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: June 8, 2016
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.