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Protein

Ferritin BfrB

Gene

bfrB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron-storage protein that displays ferroxidase activity, catalyzing the oxidation of Fe2+ ions into Fe3+ ions, that can then be deposited as a ferric-oxide mineral core within the central cavity of the protein complex.1 Publication

Catalytic activityi

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi22Iron 1PROSITE-ProRule annotation1
Metal bindingi55Iron 1PROSITE-ProRule annotation1
Metal bindingi55Iron 2PROSITE-ProRule annotation1
Metal bindingi58Iron 1PROSITE-ProRule annotation1
Metal bindingi99Iron 2PROSITE-ProRule annotation1
Metal bindingi132Iron 2PROSITE-ProRule annotation1

GO - Molecular functioni

  • ferric iron binding Source: InterPro
  • ferroxidase activity Source: MTBBASE
  • iron ion binding Source: GO_Central

GO - Biological processi

  • intracellular sequestering of iron ion Source: MTBBASE
  • iron assimilation by chelation and transport Source: Reactome
  • iron ion transport Source: InterPro
  • response to hypoxia Source: MTBBASE
  • response to nitrosative stress Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Iron storage

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-8137-MONOMER.
ReactomeiR-HSA-1222449. Mtb iron assimilation by chelation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferritin BfrB (EC:1.16.3.1)
Alternative name(s):
Non-heme ferritin Ftn
Nox19
Gene namesi
Name:bfrB
Synonyms:ftn
Ordered Locus Names:Rv3841
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3841.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular region Source: MTBBASE
  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00003929422 – 181Ferritin BfrBAdd BLAST180

Proteomic databases

PaxDbiP9WNE5.

Expressioni

Inductioni

Induced by 1 mM reactive nitrogen intermediate sodium nitroprusside dihydrate (at protein level). Induced by exposure to 50 µM FeCl3. A possible member of the dormancy regulon. Induced in response to reduced oxygen tension (hypoxia), repressed by carbon monoxide (CO). It is hoped that this regulon will give insight into the latent, or dormant phase of infection.4 Publications

Interactioni

Subunit structurei

Homooligomer of 24 subunits that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be stored.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv3841.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 38Combined sources29
Helixi42 – 68Combined sources27
Helixi88 – 115Combined sources28
Helixi119 – 149Combined sources31
Helixi153 – 163Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QD8X-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-181[»]
3UNOX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-181[»]
ProteinModelPortaliP9WNE5.
SMRiP9WNE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 150Ferritin-like diironPROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ferritin family. Prokaryotic subfamily.Curated
Contains 1 ferritin-like diiron domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107YEV. Bacteria.
COG1528. LUCA.
OMAiQYFLDRD.
PhylomeDBiP9WNE5.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR001519. Ferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PANTHERiPTHR11431. PTHR11431. 1 hit.
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WNE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYEGPKTK FHALMQEQIH NEFTAAQQYV AIAVYFDSED LPQLAKHFYS
60 70 80 90 100
QAVEERNHAM MLVQHLLDRD LRVEIPGVDT VRNQFDRPRE ALALALDQER
110 120 130 140 150
TVTDQVGRLT AVARDEGDFL GEQFMQWFLQ EQIEEVALMA TLVRVADRAG
160 170 180
ANLFELENFV AREVDVAPAA SGAPHAAGGR L
Length:181
Mass (Da):20,442
Last modified:April 16, 2014 - v1
Checksum:iC0F375669A292F55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102693 Genomic DNA. Translation: AAF06357.1.
AL123456 Genomic DNA. Translation: CCP46670.1.
PIRiF70653.
RefSeqiNP_218358.1. NC_000962.3.
WP_003420920.1. NZ_KK339374.1.

Genome annotation databases

EnsemblBacteriaiCCP46670; CCP46670; Rv3841.
GeneIDi886176.
KEGGimtu:Rv3841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102693 Genomic DNA. Translation: AAF06357.1.
AL123456 Genomic DNA. Translation: CCP46670.1.
PIRiF70653.
RefSeqiNP_218358.1. NC_000962.3.
WP_003420920.1. NZ_KK339374.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QD8X-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-181[»]
3UNOX-ray2.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-181[»]
ProteinModelPortaliP9WNE5.
SMRiP9WNE5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3841.

Proteomic databases

PaxDbiP9WNE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46670; CCP46670; Rv3841.
GeneIDi886176.
KEGGimtu:Rv3841.

Organism-specific databases

TubercuListiRv3841.

Phylogenomic databases

eggNOGiENOG4107YEV. Bacteria.
COG1528. LUCA.
OMAiQYFLDRD.
PhylomeDBiP9WNE5.

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-8137-MONOMER.
ReactomeiR-HSA-1222449. Mtb iron assimilation by chelation.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR001519. Ferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PANTHERiPTHR11431. PTHR11431. 1 hit.
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBFRB_MYCTU
AccessioniPrimary (citable) accession number: P9WNE5
Secondary accession number(s): L0TGW6
, P96237, Q7BS08, Q7D4R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.