Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

F420-dependent glucose-6-phosphate dehydrogenase

Gene

fgd1

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the coenzyme F420-dependent oxidation of glucose 6-phosphate to 6-phosphogluconolactone (PubMed:18434308). Appears to have a role in resistance to oxidative stress, via its consumption of G6P that serves as a source of reducing power to combat oxidative stress in mycobacteria. More precisely, is likely involved in a F420-dependent anti-oxidant mechanism that protects M.tuberculosis against oxidative stress and bactericidal agents (PubMed:23240649).UniRule annotation1 Publication1 Publication
Is essential for the bioreductive activation of the bicyclic 4-nitroimidazole prodrug PA-824 (a nitroimidazo-oxazine) developed for anti-tuberculosis therapy against both replicating and persistent bacteria. It does not interact directly with PA-824 but, rather, provides reduced F420 to the deazaflavin-dependent nitroreductase Ddn, which in turn activates PA-824.1 Publication

Catalytic activityi

D-glucose 6-phosphate + oxidized coenzyme F420 = D-glucono-1,5-lactone 6-phosphate + reduced coenzyme F420.1 Publication

Enzyme regulationi

Inhibited by citrate.1 Publication

Kineticsi

  1. KM=100 µM for glucose 6-phosphate1 Publication

    pH dependencei

    Optimum pH is 6.5-7.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei39F420Combined sources1 Publication1
    Active sitei40Proton donor1 Publication1
    Binding sitei76F4201 Publication1
    Active sitei109Proton acceptor1 Publication1
    Binding sitei112F420Combined sources1 Publication1
    Binding sitei195Substrate1 Publication1
    Binding sitei198Substrate1 Publication1
    Binding sitei259Substrate1 Publication1
    Binding sitei283Substrate1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • carbohydrate metabolic process Source: UniProtKB-HAMAP
    • cell redox homeostasis Source: Reactome
    • oxidation-reduction process Source: MTBBASE
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:G185E-4532-MONOMER.
    MTBH37RV:G185E-4532-MONOMER.
    ReactomeiR-HSA-1222541. Cell redox homeostasis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    F420-dependent glucose-6-phosphate dehydrogenase1 Publication (EC:1.1.98.21 Publication)
    Short name:
    FGD
    Short name:
    FGD11 Publication
    Short name:
    G6PD
    Gene namesi
    Name:fgd1
    Synonyms:fgd
    Ordered Locus Names:Rv0407
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    Proteomesi
    • UP000001584 Componenti: Chromosome

    Organism-specific databases

    TubercuListiRv0407.

    Subcellular locationi

    GO - Cellular componenti

    • cell wall Source: MTBBASE
    • cytosol Source: Reactome
    • plasma membrane Source: MTBBASE
    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene display a strong resistance to PA-824 and CGI-17341 (a nitroimidazo-oxazole).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003995041 – 336F420-dependent glucose-6-phosphate dehydrogenaseAdd BLAST336

    Proteomic databases

    PaxDbiP9WNE1.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    STRINGi83332.Rv0407.

    Structurei

    Secondary structure

    1336
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 10Combined sources6
    Turni12 – 14Combined sources3
    Helixi17 – 29Combined sources13
    Beta strandi33 – 37Combined sources5
    Helixi54 – 64Combined sources11
    Beta strandi69 – 73Combined sources5
    Turni77 – 80Combined sources4
    Helixi83 – 96Combined sources14
    Beta strandi101 – 105Combined sources5
    Helixi110 – 113Combined sources4
    Turni114 – 117Combined sources4
    Helixi125 – 142Combined sources18
    Beta strandi148 – 151Combined sources4
    Beta strandi156 – 160Combined sources5
    Beta strandi172 – 175Combined sources4
    Helixi179 – 188Combined sources10
    Beta strandi190 – 195Combined sources6
    Helixi200 – 205Combined sources6
    Helixi207 – 217Combined sources11
    Helixi222 – 224Combined sources3
    Beta strandi225 – 235Combined sources11
    Helixi239 – 244Combined sources6
    Helixi245 – 253Combined sources9
    Helixi256 – 261Combined sources6
    Helixi265 – 273Combined sources9
    Helixi277 – 281Combined sources5
    Beta strandi284 – 289Combined sources6
    Helixi290 – 302Combined sources13
    Beta strandi307 – 311Combined sources5
    Helixi317 – 327Combined sources11
    Helixi329 – 333Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3B4YX-ray1.95A/B1-336[»]
    3C8NX-ray1.90A/B/C/D1-336[»]
    ProteinModelPortaliP9WNE1.
    SMRiP9WNE1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni107 – 108F420 bindingCombined sources1 Publication2
    Regioni177 – 178F420 bindingCombined sources1 Publication2
    Regioni180 – 181F420 binding1 Publication2

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG41079Y2. Bacteria.
    COG2141. LUCA.
    KOiK15510.
    OMAiGKGMDLY.
    PhylomeDBiP9WNE1.

    Family and domain databases

    Gene3Di3.20.20.30. 1 hit.
    HAMAPiMF_02123. F420_G6P_DH. 1 hit.
    InterProiIPR019944. F420-dep_G6P_DH.
    IPR019945. F420_G6P_DH-rel.
    IPR011251. Luciferase-like_dom.
    [Graphical view]
    PfamiPF00296. Bac_luciferase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51679. SSF51679. 1 hit.
    TIGRFAMsiTIGR03554. F420_G6P_DH. 1 hit.
    TIGR03557. F420_G6P_family. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P9WNE1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAELKLGYKA SAEQFAPREL VELAVAAEAH GMDSATVSDH FQPWRHQGGH
    60 70 80 90 100
    APFSLSWMTA VGERTNRLLL GTSVLTPTFR YNPAVIAQAF ATMGCLYPNR
    110 120 130 140 150
    VFLGVGTGEA LNEIATGYEG AWPEFKERFA RLRESVGLMR QLWSGDRVDF
    160 170 180 190 200
    DGDYYRLKGA SIYDVPDGGV PVYIAAGGPA VAKYAGRAGD GFICTSGKGE
    210 220 230 240 250
    ELYTEKLMPA VREGAAAADR SVDGIDKMIE IKISYDPDPE LALNNTRFWA
    260 270 280 290 300
    PLSLTAEQKH SIDDPIEMEK AADALPIEQI AKRWIVASDP DEAVEKVGQY
    310 320 330
    VTWGLNHLVF HAPGHDQRRF LELFQSDLAP RLRRLG
    Length:336
    Mass (Da):36,989
    Last modified:April 16, 2014 - v1
    Checksum:iA25059725E3A60B3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP43138.1.
    PIRiE70628.
    RefSeqiNP_214921.1. NC_000962.3.
    WP_003898438.1. NZ_KK339370.1.

    Genome annotation databases

    EnsemblBacteriaiCCP43138; CCP43138; Rv0407.
    GeneIDi886418.
    KEGGimtu:Rv0407.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP43138.1.
    PIRiE70628.
    RefSeqiNP_214921.1. NC_000962.3.
    WP_003898438.1. NZ_KK339370.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3B4YX-ray1.95A/B1-336[»]
    3C8NX-ray1.90A/B/C/D1-336[»]
    ProteinModelPortaliP9WNE1.
    SMRiP9WNE1.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi83332.Rv0407.

    Proteomic databases

    PaxDbiP9WNE1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCCP43138; CCP43138; Rv0407.
    GeneIDi886418.
    KEGGimtu:Rv0407.

    Organism-specific databases

    TubercuListiRv0407.

    Phylogenomic databases

    eggNOGiENOG41079Y2. Bacteria.
    COG2141. LUCA.
    KOiK15510.
    OMAiGKGMDLY.
    PhylomeDBiP9WNE1.

    Enzyme and pathway databases

    BioCyciMetaCyc:G185E-4532-MONOMER.
    MTBH37RV:G185E-4532-MONOMER.
    ReactomeiR-HSA-1222541. Cell redox homeostasis.

    Family and domain databases

    Gene3Di3.20.20.30. 1 hit.
    HAMAPiMF_02123. F420_G6P_DH. 1 hit.
    InterProiIPR019944. F420-dep_G6P_DH.
    IPR019945. F420_G6P_DH-rel.
    IPR011251. Luciferase-like_dom.
    [Graphical view]
    PfamiPF00296. Bac_luciferase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51679. SSF51679. 1 hit.
    TIGRFAMsiTIGR03554. F420_G6P_DH. 1 hit.
    TIGR03557. F420_G6P_family. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiFGD_MYCTU
    AccessioniPrimary (citable) accession number: P9WNE1
    Secondary accession number(s): L0T6D5, P96253, Q7D9V4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: November 2, 2016
    This is version 21 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.