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Protein

Dihydroneopterin aldolase

Gene

folB

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin, 7,8-dihydromonapterin and 7,8-dihydroxanthopterin, respectively, in equal quantities. After longer incubation times the only product is 6-hydroxymethyl-7,8-dihydropterin.By similarity

Catalytic activityi

7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde.By similarity
7,8-dihydroneopterin = 7,8-dihydromonapterin.By similarity
7,8-dihydroneopterin + O2 = 7,8-dihydroxanthopterin + formate + glycolaldehyde.By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Dihydroneopterin aldolase (folB)
  4. 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (folK)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22SubstrateBy similarity1
Binding sitei54SubstrateBy similarity1
Active sitei99Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Oxidoreductase
Biological processFolate biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00077; UER00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroneopterin aldolase (EC:4.1.2.25By similarity)
Short name:
DHNA
Alternative name(s):
7,8-dihydroneopterin 2'-epimerase
7,8-dihydroneopterin aldolase
7,8-dihydroneopterin epimerase (EC:5.1.99.8By similarity)
7,8-dihydroneopterin hydroxylase (EC:1.13.11.81By similarity)
Dihydroneopterin epimerase
Dihydroneopterin hydroxylase
Gene namesi
Name:folB
Ordered Locus Names:MT3712.1
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001020 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004271511 – 133Dihydroneopterin aldolaseAdd BLAST133

Structurei

3D structure databases

ProteinModelPortaliP9WNC4.
SMRiP9WNC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 74Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the DHNA family.Curated

Phylogenomic databases

KOiK01633.
OrthoDBiPOG091H02IN.

Family and domain databases

CDDicd00534. DHNA_DHNTPE. 1 hit.
InterProiView protein in InterPro
IPR006156. Dihydroneopterin_aldolase.
IPR006157. FolB_dom.
PfamiView protein in Pfam
PF02152. FolB. 1 hit.
SMARTiView protein in SMART
SM00905. FolB. 1 hit.
TIGRFAMsiTIGR00525. folB. 1 hit.
TIGR00526. folB_dom. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WNC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRIELRGL TVHGRHGVYD HERVAGQRFV IDVTVWIDLA EAANSDDLAD
60 70 80 90 100
TYDYVRLASR AAEIVAGPPR KLIETVGAEI ADHVMDDQRV HAVEVAVHKP
110 120 130
QAPIPQTFDD VAVVIRRSRR GGRGWVVPAG GAV
Length:133
Mass (Da):14,552
Last modified:April 16, 2014 - v1
Checksum:iB7BD7CD0D71AFB54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK48070.1.
PIRiH70955.
RefSeqiWP_003419537.1. NZ_KK341227.1.

Genome annotation databases

EnsemblBacteriaiAAK48070; AAK48070; MT3712.1.
KEGGimtc:MT3712.1.
PATRICifig|83331.31.peg.3995.

Similar proteinsi

Entry informationi

Entry nameiFOLB_MYCTO
AccessioniPrimary (citable) accession number: P9WNC4
Secondary accession number(s): L0TG79, O06275, P0A580
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: June 7, 2017
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families