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Protein

Fumarate reductase iron-sulfur subunit

Gene

frdB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi61 – 611Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi76 – 761Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi149 – 1491Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi152 – 1521Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi159 – 1591Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi205 – 2051Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi211 – 2111Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi215 – 2151Iron-sulfur 2 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  4. electron carrier activity Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
  6. succinate dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate reductase iron-sulfur subunit (EC:1.3.5.1)
Gene namesi
Name:frdB
Ordered Locus Names:Rv1553
ORF Names:MTCY48.12c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv1553.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247Fumarate reductase iron-sulfur subunitPRO_0000158705Add
BLAST

Interactioni

Subunit structurei

Fumarate dehydrogenase forms part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, and two hydrophobic anchor proteins.By similarity

Structurei

3D structure databases

ProteinModelPortaliP9WN89.
SMRiP9WN89. Positions 2-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 94812Fe-2S ferredoxin-typeAdd
BLAST
Domaini140 – 169304Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK00245.
OMAiVGACSEV.
PhylomeDBiP9WN89.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
PF00037. Fer4. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WN89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMDRIVMEVS RYRPEIESAP TFQAYEVPLT REWAVLDGLT YIKDHLDGTL
60 70 80 90 100
SFRWSCRMGI CGSSGMTING DPKLACATFL ADYLPGPVRV EPMRNFPVIR
110 120 130 140 150
DLVVDISDFM AKLPSVKPWL VRHDEPPVED GEYRQTPAEL DAFKQFSMCI
160 170 180 190 200
NCMLCYSACP VYALDPDFLG PAAIALGQRY NLDSRDQGAA DRRDVLAAAD
210 220 230 240
GAWACTLVGE CSTACPKGVD PAGAIQRYKL TAATHALKKL LFPWGGG
Length:247
Mass (Da):27,234
Last modified:April 16, 2014 - v1
Checksum:i15863DCCB2C82B0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44317.1.
PIRiF70762.
RefSeqiNP_216069.1. NC_000962.3.
YP_006514942.1. NC_018143.2.

Genome annotation databases

EnsemblBacteriaiCCP44317; CCP44317; Rv1553.
GeneIDi13316331.
886378.
KEGGimtu:Rv1553.
mtv:RVBD_1553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44317.1.
PIRiF70762.
RefSeqiNP_216069.1. NC_000962.3.
YP_006514942.1. NC_018143.2.

3D structure databases

ProteinModelPortaliP9WN89.
SMRiP9WN89. Positions 2-243.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP44317; CCP44317; Rv1553.
GeneIDi13316331.
886378.
KEGGimtu:Rv1553.
mtv:RVBD_1553.

Organism-specific databases

TubercuListiRv1553.

Phylogenomic databases

KOiK00245.
OMAiVGACSEV.
PhylomeDBiP9WN89.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
PF00037. Fer4. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiFRDB_MYCTU
AccessioniPrimary (citable) accession number: P9WN89
Secondary accession number(s): L0T766, Q10761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 4, 2015
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.