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Protein

Glucose-6-phosphate isomerase

Gene

pgi

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA), Putative ATP-dependent 6-phosphofructokinase isozyme 2 (pfkB)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei357Proton donorBy similarity1
Active sitei388By similarity1
Active sitei514By similarity1

GO - Molecular functioni

  • glucose-6-phosphate isomerase activity Source: MTBBASE

GO - Biological processi

  • gluconeogenesis Source: UniProtKB-HAMAP
  • glycolytic process Source: MTBBASE
  • growth Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-5101-MONOMER.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgi
Ordered Locus Names:Rv0946c
ORF Names:MTCY10D7.28
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0946c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001806772 – 553Glucose-6-phosphate isomeraseAdd BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP9WN69.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0946c.

Structurei

Secondary structure

1553
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Helixi13 – 25Combined sources13
Helixi30 – 36Combined sources7
Helixi40 – 43Combined sources4
Beta strandi44 – 48Combined sources5
Beta strandi51 – 54Combined sources4
Beta strandi57 – 59Combined sources3
Helixi62 – 74Combined sources13
Helixi77 – 86Combined sources10
Turni92 – 95Combined sources4
Helixi100 – 104Combined sources5
Helixi118 – 138Combined sources21
Beta strandi144 – 146Combined sources3
Beta strandi151 – 155Combined sources5
Helixi158 – 160Combined sources3
Helixi162 – 170Combined sources9
Helixi172 – 174Combined sources3
Beta strandi180 – 184Combined sources5
Helixi189 – 196Combined sources8
Helixi201 – 203Combined sources3
Beta strandi204 – 209Combined sources6
Beta strandi211 – 213Combined sources3
Helixi216 – 233Combined sources18
Helixi235 – 240Combined sources6
Beta strandi242 – 245Combined sources4
Helixi249 – 252Combined sources4
Turni253 – 256Combined sources4
Helixi259 – 261Combined sources3
Helixi271 – 273Combined sources3
Turni275 – 277Combined sources3
Helixi278 – 280Combined sources3
Helixi281 – 287Combined sources7
Helixi289 – 308Combined sources20
Helixi311 – 313Combined sources3
Helixi315 – 329Combined sources15
Beta strandi334 – 340Combined sources7
Helixi342 – 344Combined sources3
Helixi347 – 359Combined sources13
Beta strandi362 – 367Combined sources6
Beta strandi377 – 379Combined sources3
Turni383 – 385Combined sources3
Helixi386 – 388Combined sources3
Helixi391 – 396Combined sources6
Beta strandi403 – 410Combined sources8
Helixi424 – 441Combined sources18
Helixi445 – 449Combined sources5
Turni450 – 452Combined sources3
Helixi455 – 461Combined sources7
Beta strandi469 – 476Combined sources8
Helixi479 – 500Combined sources22
Helixi508 – 510Combined sources3
Helixi511 – 525Combined sources15
Beta strandi526 – 528Combined sources3
Helixi536 – 548Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WU8X-ray2.25A7-553[»]
ProteinModelPortaliP9WN69.
SMRiP9WN69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiENOG4107QP8. Bacteria.
COG0166. LUCA.
KOiK01810.
OMAiWLLEHSK.
PhylomeDBiP9WN69.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WN69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSAPIPDIT ATPAWDALRR HHDQIGNTHL RQFFADDPGR GRELTVSVGD
60 70 80 90 100
LYIDYSKHRV TRETLALLID LARTAHLEER RDQMFAGVHI NTSEDRAVLH
110 120 130 140 150
TALRLPRDAE LVVDGQDVVT DVHAVLDAMG AFTDRLRSGE WTGATGKRIS
160 170 180 190 200
TVVNIGIGGS DLGPVMVYQA LRHYADAGIS ARFVSNVDPA DLIATLADLD
210 220 230 240 250
PATTLFIVAS KTFSTLETLT NATAARRWLT DALGDAAVSR HFVAVSTNKR
260 270 280 290 300
LVDDFGINTD NMFGFWDWVG GRYSVDSAIG LSLMTVIGRD AFADFLAGFH
310 320 330 340 350
IIDRHFATAP LESNAPVLLG LIGLWYSNFF GAQSRTVLPY SNDLSRFPAY
360 370 380 390 400
LQQLTMESNG KSTRADGSPV SADTGEIFWG EPGTNGQHAF YQLLHQGTRL
410 420 430 440 450
VPADFIGFAQ PLDDLPTAEG TGSMHDLLMS NFFAQTQVLA FGKTAEEIAA
460 470 480 490 500
DGTPAHVVAH KVMPGNRPST SILASRLTPS VLGQLIALYE HQVFTEGVVW
510 520 530 540 550
GIDSFDQWGV ELGKTQAKAL LPVITGAGSP PPQSDSSTDG LVRRYRTERG

RAG
Length:553
Mass (Da):59,974
Last modified:April 16, 2014 - v1
Checksum:iFB57DFFD16386AE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43694.1.
PIRiH70715.
RefSeqiNP_215461.1. NC_000962.3.
WP_003404830.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP43694; CCP43694; Rv0946c.
GeneIDi885533.
KEGGimtu:Rv0946c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43694.1.
PIRiH70715.
RefSeqiNP_215461.1. NC_000962.3.
WP_003404830.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WU8X-ray2.25A7-553[»]
ProteinModelPortaliP9WN69.
SMRiP9WN69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv0946c.

Proteomic databases

PaxDbiP9WN69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP43694; CCP43694; Rv0946c.
GeneIDi885533.
KEGGimtu:Rv0946c.

Organism-specific databases

TubercuListiRv0946c.

Phylogenomic databases

eggNOGiENOG4107QP8. Bacteria.
COG0166. LUCA.
KOiK01810.
OMAiWLLEHSK.
PhylomeDBiP9WN69.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
BioCyciMTBH37RV:G185E-5101-MONOMER.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase. 1 hit.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PI_MYCTU
AccessioniPrimary (citable) accession number: P9WN69
Secondary accession number(s): L0T6W0, P64192, P77895
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.