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Protein

Glucose-6-phosphate isomerase

Gene

pgi

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (LH57_05150), Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), 6-phosphofructokinase (LH57_16435), Putative ATP-dependent 6-phosphofructokinase isozyme 2 (pfkB)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei357 – 3571Proton donorBy similarity
Active sitei388 – 3881By similarity
Active sitei514 – 5141By similarity

GO - Molecular functioni

  • glucose-6-phosphate isomerase activity Source: MTBBASE

GO - Biological processi

  • gluconeogenesis Source: UniProtKB-HAMAP
  • glycolytic process Source: MTBBASE
  • growth Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgi
Ordered Locus Names:Rv0946c
ORF Names:MTCY10D7.28
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0946c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 553552Glucose-6-phosphate isomerasePRO_0000180677Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP9WN69.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0946c.

Structurei

Secondary structure

1
553
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 113Combined sources
Helixi13 – 2513Combined sources
Helixi30 – 367Combined sources
Helixi40 – 434Combined sources
Beta strandi44 – 485Combined sources
Beta strandi51 – 544Combined sources
Beta strandi57 – 593Combined sources
Helixi62 – 7413Combined sources
Helixi77 – 8610Combined sources
Turni92 – 954Combined sources
Helixi100 – 1045Combined sources
Helixi118 – 13821Combined sources
Beta strandi144 – 1463Combined sources
Beta strandi151 – 1555Combined sources
Helixi158 – 1603Combined sources
Helixi162 – 1709Combined sources
Helixi172 – 1743Combined sources
Beta strandi180 – 1845Combined sources
Helixi189 – 1968Combined sources
Helixi201 – 2033Combined sources
Beta strandi204 – 2096Combined sources
Beta strandi211 – 2133Combined sources
Helixi216 – 23318Combined sources
Helixi235 – 2406Combined sources
Beta strandi242 – 2454Combined sources
Helixi249 – 2524Combined sources
Turni253 – 2564Combined sources
Helixi259 – 2613Combined sources
Helixi271 – 2733Combined sources
Turni275 – 2773Combined sources
Helixi278 – 2803Combined sources
Helixi281 – 2877Combined sources
Helixi289 – 30820Combined sources
Helixi311 – 3133Combined sources
Helixi315 – 32915Combined sources
Beta strandi334 – 3407Combined sources
Helixi342 – 3443Combined sources
Helixi347 – 35913Combined sources
Beta strandi362 – 3676Combined sources
Beta strandi377 – 3793Combined sources
Turni383 – 3853Combined sources
Helixi386 – 3883Combined sources
Helixi391 – 3966Combined sources
Beta strandi403 – 4108Combined sources
Helixi424 – 44118Combined sources
Helixi445 – 4495Combined sources
Turni450 – 4523Combined sources
Helixi455 – 4617Combined sources
Beta strandi469 – 4768Combined sources
Helixi479 – 50022Combined sources
Helixi508 – 5103Combined sources
Helixi511 – 52515Combined sources
Beta strandi526 – 5283Combined sources
Helixi536 – 54813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WU8X-ray2.25A7-553[»]
ProteinModelPortaliP9WN69.
SMRiP9WN69. Positions 7-553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Phylogenomic databases

eggNOGiENOG4107QP8. Bacteria.
COG0166. LUCA.
KOiK01810.
OMAiCETQAML.
PhylomeDBiP9WN69.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WN69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSAPIPDIT ATPAWDALRR HHDQIGNTHL RQFFADDPGR GRELTVSVGD
60 70 80 90 100
LYIDYSKHRV TRETLALLID LARTAHLEER RDQMFAGVHI NTSEDRAVLH
110 120 130 140 150
TALRLPRDAE LVVDGQDVVT DVHAVLDAMG AFTDRLRSGE WTGATGKRIS
160 170 180 190 200
TVVNIGIGGS DLGPVMVYQA LRHYADAGIS ARFVSNVDPA DLIATLADLD
210 220 230 240 250
PATTLFIVAS KTFSTLETLT NATAARRWLT DALGDAAVSR HFVAVSTNKR
260 270 280 290 300
LVDDFGINTD NMFGFWDWVG GRYSVDSAIG LSLMTVIGRD AFADFLAGFH
310 320 330 340 350
IIDRHFATAP LESNAPVLLG LIGLWYSNFF GAQSRTVLPY SNDLSRFPAY
360 370 380 390 400
LQQLTMESNG KSTRADGSPV SADTGEIFWG EPGTNGQHAF YQLLHQGTRL
410 420 430 440 450
VPADFIGFAQ PLDDLPTAEG TGSMHDLLMS NFFAQTQVLA FGKTAEEIAA
460 470 480 490 500
DGTPAHVVAH KVMPGNRPST SILASRLTPS VLGQLIALYE HQVFTEGVVW
510 520 530 540 550
GIDSFDQWGV ELGKTQAKAL LPVITGAGSP PPQSDSSTDG LVRRYRTERG

RAG
Length:553
Mass (Da):59,974
Last modified:April 16, 2014 - v1
Checksum:iFB57DFFD16386AE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43694.1.
PIRiH70715.
RefSeqiNP_215461.1. NC_000962.3.
WP_003404830.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP43694; CCP43694; Rv0946c.
GeneIDi885533.
KEGGimtu:Rv0946c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43694.1.
PIRiH70715.
RefSeqiNP_215461.1. NC_000962.3.
WP_003404830.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WU8X-ray2.25A7-553[»]
ProteinModelPortaliP9WN69.
SMRiP9WN69. Positions 7-553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv0946c.

Proteomic databases

PaxDbiP9WN69.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP43694; CCP43694; Rv0946c.
GeneIDi885533.
KEGGimtu:Rv0946c.

Organism-specific databases

TubercuListiRv0946c.

Phylogenomic databases

eggNOGiENOG4107QP8. Bacteria.
COG0166. LUCA.
KOiK01810.
OMAiCETQAML.
PhylomeDBiP9WN69.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiG6PI_MYCTU
AccessioniPrimary (citable) accession number: P9WN69
Secondary accession number(s): L0T6W0, P64192, P77895
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 11, 2015
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.