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P9WN49

- GLMS_MYCTU

UniProt

P9WN49 - GLMS_MYCTU

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Protein
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
Gene
glmS, Rv3436c, MTCY77.08c
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activity By similarity
Active sitei619 – 6191For Fru-6P isomerization activity By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase
Gene namesi
Name:glmS
Ordered Locus Names:Rv3436c
ORF Names:MTCY77.08c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv3436c.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 624623Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation
PRO_0000135357Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP9WN49.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 225224Glutamine amidotransferase type-2
Add
BLAST
Domaini297 – 436140SIS 1
Add
BLAST
Domaini469 – 614146SIS 2
Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

KOiK00820.
PhylomeDBiP9WN49.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WN49-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIVGYVGR RPAYVVVMDA LRRMEYRGYD SSGIALVDGG TLTVRRRAGR    50
LANLEEAVAE MPSTALSGTT GLGHTRWATH GRPTDRNAHP HRDAAGKIAV 100
VHNGIIENFA VLRRELETAG VEFASDTDTE VAAHLVARAY RHGETADDFV 150
GSVLAVLRRL EGHFTLVFAN ADDPGTLVAA RRSTPLVLGI GDNEMFVGSD 200
VAAFIEHTRE AVELGQDQAV VITADGYRIS DFDGNDGLQA GRDFRPFHID 250
WDLAAAEKGG YEYFMLKEIA EQPAAVADTL LGHFVGGRIV LDEQRLSDQE 300
LREIDKVFVV ACGTAYHSGL LAKYAIEHWT RLPVEVELAS EFRYRDPVLD 350
RSTLVVAISQ SGETADTLEA VRHAKEQKAK VLAICNTNGS QIPRECDAVL 400
YTRAGPEIGV ASTKTFLAQI AANYLLGLAL AQARGTKYPD EVEREYHELE 450
AMPDLVARVI AATGPVAELA HRFAQSSTVL FLGRHVGYPV ALEGALKLKE 500
LAYMHAEGFA AGELKHGPIA LIEDGLPVIV VMPSPKGSAT LHAKLLSNIR 550
EIQTRGAVTI VIAEEGDETV RPYADHLIEI PAVSTLLQPL LSTIPLQVFA 600
ASVARARGYD VDKPRNLAKS VTVE 624
Length:624
Mass (Da):67,572
Last modified:April 16, 2014 - v1
Checksum:iABDFAE5F55549916
GO

Sequence cautioni

The sequence CAA04007.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF002814 Genomic DNA. Translation: AAB69988.1.
AJ000333 Genomic DNA. Translation: CAA04007.1. Different initiation.
AL123456 Genomic DNA. Translation: CCP46258.1.
PIRiB70976.
RefSeqiNP_217953.1. NC_000962.3.
YP_006516925.1. NC_018143.2.

Genome annotation databases

GeneIDi13317043.
887568.
KEGGimtu:Rv3436c.
mtv:RVBD_3436c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF002814 Genomic DNA. Translation: AAB69988.1 .
AJ000333 Genomic DNA. Translation: CAA04007.1 . Different initiation.
AL123456 Genomic DNA. Translation: CCP46258.1 .
PIRi B70976.
RefSeqi NP_217953.1. NC_000962.3.
YP_006516925.1. NC_018143.2.

3D structure databases

ProteinModelPortali P9WN49.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13317043.
887568.
KEGGi mtu:Rv3436c.
mtv:RVBD_3436c.

Organism-specific databases

TubercuListi Rv3436c.

Phylogenomic databases

KOi K00820.
PhylomeDBi P9WN49.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00164. GlmS.
InterProi IPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biochemical and genetic definition of effects of mannosamine on mycobacteria."
    Schaeffer M.L., Besra G.S., Belisle J.T., Inamine J.M.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "Structure of a recombinant glmS protein from Mycobacterium tuberculosis expressed in E. coli."
    Mckendree W.L., Schuster S.M., Richards N.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiGLMS_MYCTU
AccessioniPrimary (citable) accession number: P9WN49
Secondary accession number(s): L0TCI8
, O06253, O33274, P0A588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: September 3, 2014
This is version 5 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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