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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activityUniRule annotation1
Active sitei619For Fru-6P isomerization activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
Alternative name(s):
D-fructose-6-phosphate amidotransferaseUniRule annotation
GFATUniRule annotation
Glucosamine-6-phosphate synthaseUniRule annotation
Hexosephosphate aminotransferaseUniRule annotation
L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
Gene namesi
Name:glmSUniRule annotation
Ordered Locus Names:Rv3436c
ORF Names:MTCY77.08c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3436c.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: GO_Central
  • plasma membrane Source: MTBBASE

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00001353572 – 624Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST623

Proteomic databases

PaxDbiP9WN49.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi83332.Rv3436c.

Structurei

3D structure databases

ProteinModelPortaliP9WN49.
SMRiP9WN49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 225Glutamine amidotransferase type-2UniRule annotationAdd BLAST224
Domaini297 – 436SIS 1UniRule annotationAdd BLAST140
Domaini469 – 614SIS 2UniRule annotationAdd BLAST146

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiENOG4105C46. Bacteria.
COG0449. LUCA.
KOiK00820.
OMAiASEYRYA.
PhylomeDBiP9WN49.

Family and domain databases

CDDicd05008. SIS_GlmS_GlmD_1. 1 hit.
cd05009. SIS_GlmS_GlmD_2. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR035466. GlmS/AgaS_SIS.
IPR035490. GlmS/FrlB_SIS.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
PANTHERiPTHR10937:SF15. PTHR10937:SF15. 1 hit.
PfamiView protein in Pfam
PF01380. SIS. 2 hits.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WN49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGYVGR RPAYVVVMDA LRRMEYRGYD SSGIALVDGG TLTVRRRAGR
60 70 80 90 100
LANLEEAVAE MPSTALSGTT GLGHTRWATH GRPTDRNAHP HRDAAGKIAV
110 120 130 140 150
VHNGIIENFA VLRRELETAG VEFASDTDTE VAAHLVARAY RHGETADDFV
160 170 180 190 200
GSVLAVLRRL EGHFTLVFAN ADDPGTLVAA RRSTPLVLGI GDNEMFVGSD
210 220 230 240 250
VAAFIEHTRE AVELGQDQAV VITADGYRIS DFDGNDGLQA GRDFRPFHID
260 270 280 290 300
WDLAAAEKGG YEYFMLKEIA EQPAAVADTL LGHFVGGRIV LDEQRLSDQE
310 320 330 340 350
LREIDKVFVV ACGTAYHSGL LAKYAIEHWT RLPVEVELAS EFRYRDPVLD
360 370 380 390 400
RSTLVVAISQ SGETADTLEA VRHAKEQKAK VLAICNTNGS QIPRECDAVL
410 420 430 440 450
YTRAGPEIGV ASTKTFLAQI AANYLLGLAL AQARGTKYPD EVEREYHELE
460 470 480 490 500
AMPDLVARVI AATGPVAELA HRFAQSSTVL FLGRHVGYPV ALEGALKLKE
510 520 530 540 550
LAYMHAEGFA AGELKHGPIA LIEDGLPVIV VMPSPKGSAT LHAKLLSNIR
560 570 580 590 600
EIQTRGAVTI VIAEEGDETV RPYADHLIEI PAVSTLLQPL LSTIPLQVFA
610 620
ASVARARGYD VDKPRNLAKS VTVE
Length:624
Mass (Da):67,572
Last modified:April 16, 2014 - v1
Checksum:iABDFAE5F55549916
GO

Sequence cautioni

The sequence CAA04007 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002814 Genomic DNA. Translation: AAB69988.1.
AJ000333 Genomic DNA. Translation: CAA04007.1. Different initiation.
AL123456 Genomic DNA. Translation: CCP46258.1.
PIRiB70976.
RefSeqiNP_217953.1. NC_000962.3.
WP_003418289.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP46258; CCP46258; Rv3436c.
GeneIDi887568.
KEGGimtu:Rv3436c.

Similar proteinsi

Entry informationi

Entry nameiGLMS_MYCTU
AccessioniPrimary (citable) accession number: P9WN49
Secondary accession number(s): L0TCI8
, O06253, O33274, P0A588
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: August 30, 2017
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names