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P9WN49

- GLMS_MYCTU

UniProt

P9WN49 - GLMS_MYCTU

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 6 (01 Oct 2014)
      Sequence version 1 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Nucleophile; for GATase activityUniRule annotation
    Active sitei619 – 6191For Fru-6P isomerization activityUniRule annotation

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. glutamine metabolic process Source: UniProtKB-HAMAP
    3. growth Source: MTBBASE

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
    Alternative name(s):
    D-fructose-6-phosphate amidotransferaseUniRule annotation
    GFATUniRule annotation
    Glucosamine-6-phosphate synthaseUniRule annotation
    Hexosephosphate aminotransferaseUniRule annotation
    L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
    Gene namesi
    Name:glmSUniRule annotation
    Ordered Locus Names:Rv3436c
    ORF Names:MTCY77.08c
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001584: Chromosome

    Organism-specific databases

    TubercuListiRv3436c.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: MTBBASE

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation
    Chaini2 – 624623Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135357Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP9WN49.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 225224Glutamine amidotransferase type-2UniRule annotationAdd
    BLAST
    Domaini297 – 436140SIS 1UniRule annotationAdd
    BLAST
    Domaini469 – 614146SIS 2UniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
    Contains 2 SIS domains.UniRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    KOiK00820.
    PhylomeDBiP9WN49.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00164. GlmS.
    InterProiIPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01135. glmS. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P9WN49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIVGYVGR RPAYVVVMDA LRRMEYRGYD SSGIALVDGG TLTVRRRAGR    50
    LANLEEAVAE MPSTALSGTT GLGHTRWATH GRPTDRNAHP HRDAAGKIAV 100
    VHNGIIENFA VLRRELETAG VEFASDTDTE VAAHLVARAY RHGETADDFV 150
    GSVLAVLRRL EGHFTLVFAN ADDPGTLVAA RRSTPLVLGI GDNEMFVGSD 200
    VAAFIEHTRE AVELGQDQAV VITADGYRIS DFDGNDGLQA GRDFRPFHID 250
    WDLAAAEKGG YEYFMLKEIA EQPAAVADTL LGHFVGGRIV LDEQRLSDQE 300
    LREIDKVFVV ACGTAYHSGL LAKYAIEHWT RLPVEVELAS EFRYRDPVLD 350
    RSTLVVAISQ SGETADTLEA VRHAKEQKAK VLAICNTNGS QIPRECDAVL 400
    YTRAGPEIGV ASTKTFLAQI AANYLLGLAL AQARGTKYPD EVEREYHELE 450
    AMPDLVARVI AATGPVAELA HRFAQSSTVL FLGRHVGYPV ALEGALKLKE 500
    LAYMHAEGFA AGELKHGPIA LIEDGLPVIV VMPSPKGSAT LHAKLLSNIR 550
    EIQTRGAVTI VIAEEGDETV RPYADHLIEI PAVSTLLQPL LSTIPLQVFA 600
    ASVARARGYD VDKPRNLAKS VTVE 624
    Length:624
    Mass (Da):67,572
    Last modified:April 16, 2014 - v1
    Checksum:iABDFAE5F55549916
    GO

    Sequence cautioni

    The sequence CAA04007.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF002814 Genomic DNA. Translation: AAB69988.1.
    AJ000333 Genomic DNA. Translation: CAA04007.1. Different initiation.
    AL123456 Genomic DNA. Translation: CCP46258.1.
    PIRiB70976.
    RefSeqiNP_217953.1. NC_000962.3.
    YP_006516925.1. NC_018143.2.

    Genome annotation databases

    GeneIDi13317043.
    887568.
    KEGGimtu:Rv3436c.
    mtv:RVBD_3436c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF002814 Genomic DNA. Translation: AAB69988.1 .
    AJ000333 Genomic DNA. Translation: CAA04007.1 . Different initiation.
    AL123456 Genomic DNA. Translation: CCP46258.1 .
    PIRi B70976.
    RefSeqi NP_217953.1. NC_000962.3.
    YP_006516925.1. NC_018143.2.

    3D structure databases

    ProteinModelPortali P9WN49.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13317043.
    887568.
    KEGGi mtu:Rv3436c.
    mtv:RVBD_3436c.

    Organism-specific databases

    TubercuListi Rv3436c.

    Phylogenomic databases

    KOi K00820.
    PhylomeDBi P9WN49.

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_00164. GlmS.
    InterProi IPR017932. GATase_2_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01135. glmS. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Biochemical and genetic definition of effects of mannosamine on mycobacteria."
      Schaeffer M.L., Besra G.S., Belisle J.T., Inamine J.M.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    2. "Structure of a recombinant glmS protein from Mycobacterium tuberculosis expressed in E. coli."
      Mckendree W.L., Schuster S.M., Richards N.
      Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 25618 / H37Rv.

    Entry informationi

    Entry nameiGLMS_MYCTU
    AccessioniPrimary (citable) accession number: P9WN49
    Secondary accession number(s): L0TCI8
    , O06253, O33274, P0A588
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 6 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3