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P9WN49

- GLMS_MYCTU

UniProt

P9WN49 - GLMS_MYCTU

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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.UniRule annotation

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityUniRule annotation
Active sitei619 – 6191For Fru-6P isomerization activityUniRule annotation

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. glutamine metabolic process Source: UniProtKB-HAMAP
  3. growth Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]UniRule annotation (EC:2.6.1.16UniRule annotation)
Alternative name(s):
D-fructose-6-phosphate amidotransferaseUniRule annotation
GFATUniRule annotation
Glucosamine-6-phosphate synthaseUniRule annotation
Hexosephosphate aminotransferaseUniRule annotation
L-glutamine--D-fructose-6-phosphate amidotransferaseUniRule annotation
Gene namesi
Name:glmSUniRule annotation
Ordered Locus Names:Rv3436c
ORF Names:MTCY77.08c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv3436c.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 624623Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135357Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP9WN49.
SMRiP9WN49. Positions 2-624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 225224Glutamine amidotransferase type-2UniRule annotationAdd
BLAST
Domaini297 – 436140SIS 1UniRule annotationAdd
BLAST
Domaini469 – 614146SIS 2UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation
Contains 2 SIS domains.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

KOiK00820.
PhylomeDBiP9WN49.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WN49 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIVGYVGR RPAYVVVMDA LRRMEYRGYD SSGIALVDGG TLTVRRRAGR
60 70 80 90 100
LANLEEAVAE MPSTALSGTT GLGHTRWATH GRPTDRNAHP HRDAAGKIAV
110 120 130 140 150
VHNGIIENFA VLRRELETAG VEFASDTDTE VAAHLVARAY RHGETADDFV
160 170 180 190 200
GSVLAVLRRL EGHFTLVFAN ADDPGTLVAA RRSTPLVLGI GDNEMFVGSD
210 220 230 240 250
VAAFIEHTRE AVELGQDQAV VITADGYRIS DFDGNDGLQA GRDFRPFHID
260 270 280 290 300
WDLAAAEKGG YEYFMLKEIA EQPAAVADTL LGHFVGGRIV LDEQRLSDQE
310 320 330 340 350
LREIDKVFVV ACGTAYHSGL LAKYAIEHWT RLPVEVELAS EFRYRDPVLD
360 370 380 390 400
RSTLVVAISQ SGETADTLEA VRHAKEQKAK VLAICNTNGS QIPRECDAVL
410 420 430 440 450
YTRAGPEIGV ASTKTFLAQI AANYLLGLAL AQARGTKYPD EVEREYHELE
460 470 480 490 500
AMPDLVARVI AATGPVAELA HRFAQSSTVL FLGRHVGYPV ALEGALKLKE
510 520 530 540 550
LAYMHAEGFA AGELKHGPIA LIEDGLPVIV VMPSPKGSAT LHAKLLSNIR
560 570 580 590 600
EIQTRGAVTI VIAEEGDETV RPYADHLIEI PAVSTLLQPL LSTIPLQVFA
610 620
ASVARARGYD VDKPRNLAKS VTVE
Length:624
Mass (Da):67,572
Last modified:April 16, 2014 - v1
Checksum:iABDFAE5F55549916
GO

Sequence cautioni

The sequence CAA04007.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF002814 Genomic DNA. Translation: AAB69988.1.
AJ000333 Genomic DNA. Translation: CAA04007.1. Different initiation.
AL123456 Genomic DNA. Translation: CCP46258.1.
PIRiB70976.
RefSeqiNP_217953.1. NC_000962.3.
YP_006516925.1. NC_018143.2.

Genome annotation databases

GeneIDi13317043.
887568.
KEGGimtu:Rv3436c.
mtv:RVBD_3436c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF002814 Genomic DNA. Translation: AAB69988.1 .
AJ000333 Genomic DNA. Translation: CAA04007.1 . Different initiation.
AL123456 Genomic DNA. Translation: CCP46258.1 .
PIRi B70976.
RefSeqi NP_217953.1. NC_000962.3.
YP_006516925.1. NC_018143.2.

3D structure databases

ProteinModelPortali P9WN49.
SMRi P9WN49. Positions 2-624.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13317043.
887568.
KEGGi mtu:Rv3436c.
mtv:RVBD_3436c.

Organism-specific databases

TubercuListi Rv3436c.

Phylogenomic databases

KOi K00820.
PhylomeDBi P9WN49.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00164. GlmS.
InterProi IPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfami PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biochemical and genetic definition of effects of mannosamine on mycobacteria."
    Schaeffer M.L., Besra G.S., Belisle J.T., Inamine J.M.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "Structure of a recombinant glmS protein from Mycobacterium tuberculosis expressed in E. coli."
    Mckendree W.L., Schuster S.M., Richards N.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiGLMS_MYCTU
AccessioniPrimary (citable) accession number: P9WN49
Secondary accession number(s): L0TCI8
, O06253, O33274, P0A588
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 29, 2014
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3