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P9WMY9

- GLGSY_MYCTU

UniProt

P9WMY9 - GLGSY_MYCTU

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Protein

Glycogen synthase

Gene
Rv3032
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Glucosyltransferase that uses UDP-glucose as the sugar donor to elongate alpha-(1->4)-glucans. Is involved in the biosynthesis of both 6-O-methylglucosyl lipopolysaccharides (MGLP) and glycogen. May also use ADP-glucose as substrate.2 Publications

Catalytic activityi

UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).1 Publication

Pathwayi

GO - Molecular functioni

  1. glycogen (starch) synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen synthase (EC:2.4.1.11)
Alternative name(s):
Alpha-1,4-glucosyltransferase Rv3032
UDP-glucose--glycogen glucosyltransferase
Gene namesi
Ordered Locus Names:Rv3032
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv3032.

Pathology & Biotechi

Disruption phenotypei

Inactivation of Rv3032 affects the production of both glycogen (two-fold reduction) and 6-O-methylglucosyl lipopolysaccharides (MGLP), but not that of capsular alpha-D-glucan. Cells lacking this gene are not affected in their multiplication or persistence in the BALB/c mouse infection model. They also show a slightly slower growth than that of wild-type at 37 degrees Celsius and a completely abolished growth at 39 degrees Celsius. Moreover, in contrast to wild-type, they are exceptionally sensitive to the TreS inhibitor validamycin A; the sensitivity is abolished by overexpression of TreS.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414Glycogen synthasePRO_0000413905Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP9WMY9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK16150.
PhylomeDBiP9WMY9.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4_N.
[Graphical view]
PfamiPF13579. Glyco_trans_4_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WMY9-1 [UniParc]FASTAAdd to Basket

« Hide

MRILMVSWEY PPVVIGGLGR HVHHLSTALA AAGHDVVVLS RCPSGTDPST    50
HPSSDEVTEG VRVIAAAQDP HEFTFGNDMM AWTLAMGHAM IRAGLRLKKL 100
GTDRSWRPDV VHAHDWLVAH PAIALAQFYD VPMVSTIHAT EAGRHSGWVS 150
GALSRQVHAV ESWLVRESDS LITCSASMND EITELFGPGL AEITVIRNGI 200
DAARWPFAAR RPRTGPAELL YVGRLEYEKG VHDAIAALPR LRRTHPGTTL 250
TIAGEGTQQD WLIDQARKHR VLRATRFVGH LDHTELLALL HRADAAVLPS 300
HYEPFGLVAL EAAAAGTPLV TSNIGGLGEA VINGQTGVSC APRDVAGLAA 350
AVRSVLDDPA AAQRRARAAR QRLTSDFDWQ TVATATAQVY LAAKRGERQP 400
QPRLPIVEHA LPDR 414
Length:414
Mass (Da):44,805
Last modified:April 16, 2014 - v1
Checksum:i64ADC7A4EA17A0DE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP45840.1.
PIRiC70859.
RefSeqiNP_217548.1. NC_000962.3.
YP_006516491.1. NC_018143.2.

Genome annotation databases

GeneIDi13317834.
888185.
KEGGimtu:Rv3032.
mtv:RVBD_3032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP45840.1 .
PIRi C70859.
RefSeqi NP_217548.1. NC_000962.3.
YP_006516491.1. NC_018143.2.

3D structure databases

ProteinModelPortali P9WMY9.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13317834.
888185.
KEGGi mtu:Rv3032.
mtv:RVBD_3032.

Organism-specific databases

TubercuListi Rv3032.

Phylogenomic databases

KOi K16150.
PhylomeDBi P9WMY9.

Enzyme and pathway databases

UniPathwayi UPA00164 .

Family and domain databases

InterProi IPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4_N.
[Graphical view ]
Pfami PF13579. Glyco_trans_4_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis."
    Stadthagen G., Sambou T., Guerin M., Barilone N., Boudou F., Kordulakova J., Charles P., Alzari P.M., Lemassu A., Daffe M., Puzo G., Gicquel B., Riviere M., Jackson M.
    J. Biol. Chem. 282:27270-27276(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MGLP AND GLYCOGEN BIOSYNTHESIS, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    Strain: ATCC 25618 / H37Rv.
  3. "Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: biosynthesis and impact on the persistence in mice."
    Sambou T., Dinadayala P., Stadthagen G., Barilone N., Bordat Y., Constant P., Levillain F., Neyrolles O., Gicquel B., Lemassu A., Daffe M., Jackson M.
    Mol. Microbiol. 70:762-774(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GLYCOGEN BIOSYNTHESIS, DISRUPTION PHENOTYPE, PATHWAY.
    Strain: ATCC 25618 / H37Rv.
  4. "Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway."
    Kalscheuer R., Syson K., Veeraraghavan U., Weinrick B., Biermann K.E., Liu Z., Sacchettini J.C., Besra G., Bornemann S., Jacobs W.R. Jr.
    Nat. Chem. Biol. 6:376-384(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, SYNTHETIC LETHALITY.
    Strain: ATCC 25618 / H37Rv.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiGLGSY_MYCTU
AccessioniPrimary (citable) accession number: P9WMY9
Secondary accession number(s): L0TCY4, O53279, Q7D693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: September 3, 2014
This is version 5 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Attempts to disrupt both the Rv3032 gene and glgA in order to create a mutant simultaneously deficient in both alpha-1,4-glucosyltransferases turned out to be unsuccessful. Thus, M.tuberculosis H37Rv requires a functional copy of at least one of these two genes for growth. Moreover, it is not possible to inactivate Rv3032 in a mutant lacking treS, suggesting the joint essentiality of the different alpha-(1->4)-glucans biosynthesis pathways involving these two genes.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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