Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P9WMY9

- GLGSY_MYCTU

UniProt

P9WMY9 - GLGSY_MYCTU

Protein

Glycogen synthase

Gene

Rv3032

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 6 (01 Oct 2014)
      Sequence version 1 (16 Apr 2014)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Glucosyltransferase that uses UDP-glucose as the sugar donor to elongate alpha-(1->4)-glucans. Is involved in the biosynthesis of both 6-O-methylglucosyl lipopolysaccharides (MGLP) and glycogen. May also use ADP-glucose as substrate.2 Publications

    Catalytic activityi

    UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).1 Publication

    Pathwayi

    GO - Molecular functioni

    1. glycogen (starch) synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-UniPathway
    2. growth Source: MTBBASE

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00164.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen synthase (EC:2.4.1.11)
    Alternative name(s):
    Alpha-1,4-glucosyltransferase Rv3032
    UDP-glucose--glycogen glucosyltransferase
    Gene namesi
    Ordered Locus Names:Rv3032
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001584: Chromosome

    Organism-specific databases

    TubercuListiRv3032.

    Pathology & Biotechi

    Disruption phenotypei

    Inactivation of Rv3032 affects the production of both glycogen (two-fold reduction) and 6-O-methylglucosyl lipopolysaccharides (MGLP), but not that of capsular alpha-D-glucan. Cells lacking this gene are not affected in their multiplication or persistence in the BALB/c mouse infection model. They also show a slightly slower growth than that of wild-type at 37 degrees Celsius and a completely abolished growth at 39 degrees Celsius. Moreover, in contrast to wild-type, they are exceptionally sensitive to the TreS inhibitor validamycin A; the sensitivity is abolished by overexpression of TreS.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 414414Glycogen synthasePRO_0000413905Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP9WMY9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase group 1 family.Curated

    Phylogenomic databases

    KOiK16150.
    PhylomeDBiP9WMY9.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4_N.
    [Graphical view]
    PfamiPF13579. Glyco_trans_4_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P9WMY9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRILMVSWEY PPVVIGGLGR HVHHLSTALA AAGHDVVVLS RCPSGTDPST    50
    HPSSDEVTEG VRVIAAAQDP HEFTFGNDMM AWTLAMGHAM IRAGLRLKKL 100
    GTDRSWRPDV VHAHDWLVAH PAIALAQFYD VPMVSTIHAT EAGRHSGWVS 150
    GALSRQVHAV ESWLVRESDS LITCSASMND EITELFGPGL AEITVIRNGI 200
    DAARWPFAAR RPRTGPAELL YVGRLEYEKG VHDAIAALPR LRRTHPGTTL 250
    TIAGEGTQQD WLIDQARKHR VLRATRFVGH LDHTELLALL HRADAAVLPS 300
    HYEPFGLVAL EAAAAGTPLV TSNIGGLGEA VINGQTGVSC APRDVAGLAA 350
    AVRSVLDDPA AAQRRARAAR QRLTSDFDWQ TVATATAQVY LAAKRGERQP 400
    QPRLPIVEHA LPDR 414
    Length:414
    Mass (Da):44,805
    Last modified:April 16, 2014 - v1
    Checksum:i64ADC7A4EA17A0DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL123456 Genomic DNA. Translation: CCP45840.1.
    PIRiC70859.
    RefSeqiNP_217548.1. NC_000962.3.
    YP_006516491.1. NC_018143.2.

    Genome annotation databases

    GeneIDi13317834.
    888185.
    KEGGimtu:Rv3032.
    mtv:RVBD_3032.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL123456 Genomic DNA. Translation: CCP45840.1 .
    PIRi C70859.
    RefSeqi NP_217548.1. NC_000962.3.
    YP_006516491.1. NC_018143.2.

    3D structure databases

    ProteinModelPortali P9WMY9.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13317834.
    888185.
    KEGGi mtu:Rv3032.
    mtv:RVBD_3032.

    Organism-specific databases

    TubercuListi Rv3032.

    Phylogenomic databases

    KOi K16150.
    PhylomeDBi P9WMY9.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .

    Family and domain databases

    InterProi IPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4_N.
    [Graphical view ]
    Pfami PF13579. Glyco_trans_4_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    2. "Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis."
      Stadthagen G., Sambou T., Guerin M., Barilone N., Boudou F., Kordulakova J., Charles P., Alzari P.M., Lemassu A., Daffe M., Puzo G., Gicquel B., Riviere M., Jackson M.
      J. Biol. Chem. 282:27270-27276(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MGLP AND GLYCOGEN BIOSYNTHESIS, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
      Strain: ATCC 25618 / H37Rv.
    3. "Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: biosynthesis and impact on the persistence in mice."
      Sambou T., Dinadayala P., Stadthagen G., Barilone N., Bordat Y., Constant P., Levillain F., Neyrolles O., Gicquel B., Lemassu A., Daffe M., Jackson M.
      Mol. Microbiol. 70:762-774(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN GLYCOGEN BIOSYNTHESIS, DISRUPTION PHENOTYPE, PATHWAY.
      Strain: ATCC 25618 / H37Rv.
    4. "Self-poisoning of Mycobacterium tuberculosis by targeting GlgE in an alpha-glucan pathway."
      Kalscheuer R., Syson K., Veeraraghavan U., Weinrick B., Biermann K.E., Liu Z., Sacchettini J.C., Besra G., Bornemann S., Jacobs W.R. Jr.
      Nat. Chem. Biol. 6:376-384(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, SYNTHETIC LETHALITY.
      Strain: ATCC 25618 / H37Rv.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 25618 / H37Rv.

    Entry informationi

    Entry nameiGLGSY_MYCTU
    AccessioniPrimary (citable) accession number: P9WMY9
    Secondary accession number(s): L0TCY4, O53279, Q7D693
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 6 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Attempts to disrupt both the Rv3032 gene and glgA in order to create a mutant simultaneously deficient in both alpha-1,4-glucosyltransferases turned out to be unsuccessful. Thus, M.tuberculosis H37Rv requires a functional copy of at least one of these two genes for growth. Moreover, it is not possible to inactivate Rv3032 in a mutant lacking treS, suggesting the joint essentiality of the different alpha-(1->4)-glucans biosynthesis pathways involving these two genes.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3