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Protein

Protoporphyrinogen oxidase

Gene

hemY

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.By similarity

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase (hemY)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei242FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei390FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15FADBy similarity6
Nucleotide bindingi36 – 37FADBy similarity2
Nucleotide bindingi58 – 61FADBy similarity4
Nucleotide bindingi426 – 428FADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-6925-MONOMER.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:hemY
Ordered Locus Names:Rv2677c
ORF Names:MTV010.01c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2677c.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352651 – 452Protoporphyrinogen oxidaseAdd BLAST452

Proteomic databases

PaxDbiP9WMP1.

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity).By similarity

Protein-protein interaction databases

STRINGi83332.Rv2677c.

Structurei

3D structure databases

ProteinModelPortaliP9WMP1.
SMRiP9WMP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGiENOG4105C1Q. Bacteria.
COG1232. LUCA.
KOiK00231.
OMAiGAYLDGI.
PhylomeDBiP9WMP1.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WMP1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPRSYCVVG GGISGLTSAY RLRQAVGDDA TITLFEPADR LGGVLRTEHI
60 70 80 90 100
GGQPMDLGAE AFVLRRPEMP ALLAELGLSD RQLASTGARP LIYSQQRLHP
110 120 130 140 150
LPPQTVVGIP SSAGSMAGLV DDATLARIDA EAARPFTWQV GSDPAVADLV
160 170 180 190 200
ADRFGDQVVA RSVDPLLSGV YAGSAATIGL RAAAPSVAAA LDRGATSVTD
210 220 230 240 250
AVRQALPPGS GGPVFGALDG GYQVLLDGLV RRSRVHWVRA RVVQLERGWV
260 270 280 290 300
LRDETGGRWQ ADAVILAVPA PRLARLVDGI APRTHAAARQ IVSASSAVVA
310 320 330 340 350
LAVPGGTAFP HCSGVLVAGD ESPHAKAITL SSRKWGQRGD VALLRLSFGR
360 370 380 390 400
FGDEPALTAS DDQLLAWAAD DLVTVFGVAV DPVDVRVRRW IEAMPQYGPG
410 420 430 440 450
HADVVAELRA GLPPTLAVAG SYLDGIGVPA CVGAAGRAVT SVIEALDAQV

AR
Length:452
Mass (Da):46,846
Last modified:April 16, 2014 - v1
Checksum:i6DFA1F57BEE2810F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45475.1.
RefSeqiWP_003413871.1. NZ_KK339370.1.
YP_177675.1. NC_000962.3.

Genome annotation databases

EnsemblBacteriaiCCP45475; CCP45475; Rv2677c.
GeneIDi887711.
KEGGimtu:Rv2677c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45475.1.
RefSeqiWP_003413871.1. NZ_KK339370.1.
YP_177675.1. NC_000962.3.

3D structure databases

ProteinModelPortaliP9WMP1.
SMRiP9WMP1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2677c.

Proteomic databases

PaxDbiP9WMP1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45475; CCP45475; Rv2677c.
GeneIDi887711.
KEGGimtu:Rv2677c.

Organism-specific databases

TubercuListiRv2677c.

Phylogenomic databases

eggNOGiENOG4105C1Q. Bacteria.
COG1232. LUCA.
KOiK00231.
OMAiGAYLDGI.
PhylomeDBiP9WMP1.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
BioCyciMTBH37RV:G185E-6925-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPOX_MYCTU
AccessioniPrimary (citable) accession number: P9WMP1
Secondary accession number(s): L0TAB5, O53230, P0A5A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.