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Protein

Protoporphyrinogen oxidase

Gene

hemY

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.By similarity

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei242 – 2421FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei390 – 3901FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156FADBy similarity
Nucleotide bindingi36 – 372FADBy similarity
Nucleotide bindingi58 – 614FADBy similarity
Nucleotide bindingi426 – 4283FADBy similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:hemY
Ordered Locus Names:MT2751
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001020: Chromosome

Subcellular locationi

Cytoplasm By similarity. Membrane By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Protoporphyrinogen oxidasePRO_0000427270Add
BLAST

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP9WMP0.
SMRiP9WMP0. Positions 8-443.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

KOiK00231.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WMP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPRSYCVVG GGISGLTSAY RLRQAVGDDA TITLFEPADR LGGVLRTEHI
60 70 80 90 100
GGQPMDLGAE AFVLRRPEMP ALLAELGLSD RQLASTGARP LIYSQQRLHP
110 120 130 140 150
LPPQTVVGIP SSAGSMAGLV DDATLARIDA EAARPFTWQV GSDPAVADLV
160 170 180 190 200
ADRFGDQVVA RSVDPLLSGV YAGSAATIGL RAAAPSVAAA LDRGATSVTD
210 220 230 240 250
AVRQALPPGS GGPVFGALDG GYQVLLDGLV RRSRVHWVRA RVVQLERGWV
260 270 280 290 300
LRDETGGRWQ ADAVILAVPA PRLARLVDGI APRTHAAARQ IVSASSAVVA
310 320 330 340 350
LAVPGGTAFP HCSGVLVAGD ESPHAKAITL SSRKWGQRGD VALLRLSFGR
360 370 380 390 400
FGDEPALTAS DDQLLAWAAD DLVTVFGVAV DPVDVRVRRW IEAMPQYGPG
410 420 430 440 450
HADVVAELRA GLPPTLAVAG SYLDGIGVPA CVGAAGRAVT SVIEALDAQV

AR
Length:452
Mass (Da):46,846
Last modified:April 16, 2014 - v1
Checksum:i6DFA1F57BEE2810F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK47066.1.
RefSeqiNP_337252.1. NC_002755.2.

Genome annotation databases

EnsemblBacteriaiAAK47066; AAK47066; MT2751.
GeneIDi925547.
KEGGimtc:MT2751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK47066.1.
RefSeqiNP_337252.1. NC_002755.2.

3D structure databases

ProteinModelPortaliP9WMP0.
SMRiP9WMP0. Positions 8-443.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK47066; AAK47066; MT2751.
GeneIDi925547.
KEGGimtc:MT2751.

Phylogenomic databases

KOiK00231.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.

Family and domain databases

Gene3Di3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CDC 1551 / Oshkosh.

Entry informationi

Entry nameiPPOX_MYCTO
AccessioniPrimary (citable) accession number: P9WMP0
Secondary accession number(s): L0TAB5, O53230, P0A5A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 4, 2015
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.