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Protein

Chaperone protein DnaK

Gene

dnaK

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a chaperone.UniRule annotation
Recombinant extracellular protein activates expression of NF-kappa-B in immortalized human dermal endothelial cells in a TLR2- and TLR4-dependent manner. Activation occurs via MYD88-dependent and -independent pathways and requires TIRAP, TRIF and TRAM (some experiments done in mouse cells, mice do not usually catch tuberculosis) (PubMed:15809303).1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • fibronectin binding Source: CAFA
  • unfolded protein binding Source: InterPro
  • zymogen binding Source: CAFA

GO - Biological processi

  • cellular response to superoxide Source: MTBBASE
  • growth Source: MTBBASE
  • interaction with host via substance in symbiont surface Source: CAFA
  • positive regulation of plasminogen activation Source: CAFA
  • positive regulation of transcription regulatory region DNA binding Source: CAFA
  • protein autophosphorylation Source: MTBBASE
  • protein folding Source: InterPro
  • response to antibiotic Source: MTBBASE
  • response to copper ion Source: MTBBASE
  • response to heat Source: MTBBASE

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaK
Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70
Gene namesi
Name:dnaK
Ordered Locus Names:Rv0350
ORF Names:MTCY13E10.10
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0350

Subcellular locationi

GO - Cellular componenti

  • bacterial extracellular vesicle Source: UniProtKB
  • capsule Source: CAFA
  • cell surface Source: UniProtKB-SubCell
  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular region Source: MTBBASE
  • plasma membrane Source: MTBBASE

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000784991 – 625Chaperone protein DnaKAdd BLAST625

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei175Phosphothreonine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP9WMJ9
PRIDEiP9WMJ9

Expressioni

Inductioni

By stress conditions e.g. heat shock and oxidative stress under control of SigH. There is another promoter.1 Publication

Interactioni

GO - Molecular functioni

  • fibronectin binding Source: CAFA
  • unfolded protein binding Source: InterPro
  • zymogen binding Source: CAFA

Protein-protein interaction databases

IntActiP9WMJ9, 1 interactor
STRINGi83332.Rv0350

Structurei

3D structure databases

ProteinModelPortaliP9WMJ9
SMRiP9WMJ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiENOG4105CFG Bacteria
COG0443 LUCA
KOiK04043
OMAiISIKRHM
PhylomeDBiP9WMJ9

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
HAMAPiMF_00332 DnaK, 1 hit
InterProiView protein in InterPro
IPR012725 Chaperone_DnaK
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 2 hits
PfamiView protein in Pfam
PF00012 HSP70, 2 hits
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
TIGRFAMsiTIGR02350 prok_dnaK, 1 hit
PROSITEiView protein in PROSITE
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequencei

Sequence statusi: Complete.

P9WMJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARAVGIDLG TTNSVVSVLE GGDPVVVANS EGSRTTPSIV AFARNGEVLV
60 70 80 90 100
GQPAKNQAVT NVDRTVRSVK RHMGSDWSIE IDGKKYTAPE ISARILMKLK
110 120 130 140 150
RDAEAYLGED ITDAVITTPA YFNDAQRQAT KDAGQIAGLN VLRIVNEPTA
160 170 180 190 200
AALAYGLDKG EKEQRILVFD LGGGTFDVSL LEIGEGVVEV RATSGDNHLG
210 220 230 240 250
GDDWDQRVVD WLVDKFKGTS GIDLTKDKMA MQRLREAAEK AKIELSSSQS
260 270 280 290 300
TSINLPYITV DADKNPLFLD EQLTRAEFQR ITQDLLDRTR KPFQSVIADT
310 320 330 340 350
GISVSEIDHV VLVGGSTRMP AVTDLVKELT GGKEPNKGVN PDEVVAVGAA
360 370 380 390 400
LQAGVLKGEV KDVLLLDVTP LSLGIETKGG VMTRLIERNT TIPTKRSETF
410 420 430 440 450
TTADDNQPSV QIQVYQGERE IAAHNKLLGS FELTGIPPAP RGIPQIEVTF
460 470 480 490 500
DIDANGIVHV TAKDKGTGKE NTIRIQEGSG LSKEDIDRMI KDAEAHAEED
510 520 530 540 550
RKRREEADVR NQAETLVYQT EKFVKEQREA EGGSKVPEDT LNKVDAAVAE
560 570 580 590 600
AKAALGGSDI SAIKSAMEKL GQESQALGQA IYEAAQAASQ ATGAAHPGGE
610 620
PGGAHPGSAD DVVDAEVVDD GREAK
Length:625
Mass (Da):66,831
Last modified:April 16, 2014 - v1
Checksum:i23935906094D1A91
GO

Sequence cautioni

The sequence CAA41306 differs from that shown. Reason: Frameshift at position 552.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153 – 154LA → PG in CAA41306 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58406 Genomic DNA Translation: CAA41306.1 Frameshift.
JX026662 Genomic DNA Translation: AFM37299.1
AL123456 Genomic DNA Translation: CCP43080.1
PIRiG70574
RefSeqiNP_214864.1, NC_000962.3
WP_003401814.1, NZ_KK339370.1

Genome annotation databases

EnsemblBacteriaiCCP43080; CCP43080; Rv0350
GeneIDi885946
KEGGimtu:Rv0350

Similar proteinsi

Entry informationi

Entry nameiDNAK_MYCTU
AccessioniPrimary (citable) accession number: P9WMJ9
Secondary accession number(s): I6QM76
, O06301, P0A5B9, P32723
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: May 23, 2018
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health