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Protein

Chaperone protein DnaK

Gene

dnaK

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a chaperone.By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to superoxide Source: MTBBASE
  • growth Source: MTBBASE
  • protein autophosphorylation Source: MTBBASE
  • protein folding Source: InterPro
  • response to antibiotic Source: MTBBASE
  • response to copper ion Source: MTBBASE
  • response to heat Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaK
Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70
Gene namesi
Name:dnaK
Ordered Locus Names:Rv0350
ORF Names:MTCY13E10.10
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0350.

Subcellular locationi

GO - Cellular componenti

  • bacterial extracellular vesicle Source: UniProtKB
  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular region Source: MTBBASE
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 625625Chaperone protein DnaKPRO_0000078499Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei175 – 1751Phosphothreonine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP9WMJ9.

Expressioni

Inductioni

By stress conditions e.g. heat shock and oxidative stress under control of SigH. There is another promoter.1 Publication

Interactioni

Protein-protein interaction databases

IntActiP9WMJ9. 1 interaction.
STRINGi83332.Rv0350.

Structurei

3D structure databases

ProteinModelPortaliP9WMJ9.
SMRiP9WMJ9. Positions 1-577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiENOG4105CFG. Bacteria.
COG0443. LUCA.
KOiK04043.
OMAiEKMAPPQ.
PhylomeDBiP9WMJ9.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK. 1 hit.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WMJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARAVGIDLG TTNSVVSVLE GGDPVVVANS EGSRTTPSIV AFARNGEVLV
60 70 80 90 100
GQPAKNQAVT NVDRTVRSVK RHMGSDWSIE IDGKKYTAPE ISARILMKLK
110 120 130 140 150
RDAEAYLGED ITDAVITTPA YFNDAQRQAT KDAGQIAGLN VLRIVNEPTA
160 170 180 190 200
AALAYGLDKG EKEQRILVFD LGGGTFDVSL LEIGEGVVEV RATSGDNHLG
210 220 230 240 250
GDDWDQRVVD WLVDKFKGTS GIDLTKDKMA MQRLREAAEK AKIELSSSQS
260 270 280 290 300
TSINLPYITV DADKNPLFLD EQLTRAEFQR ITQDLLDRTR KPFQSVIADT
310 320 330 340 350
GISVSEIDHV VLVGGSTRMP AVTDLVKELT GGKEPNKGVN PDEVVAVGAA
360 370 380 390 400
LQAGVLKGEV KDVLLLDVTP LSLGIETKGG VMTRLIERNT TIPTKRSETF
410 420 430 440 450
TTADDNQPSV QIQVYQGERE IAAHNKLLGS FELTGIPPAP RGIPQIEVTF
460 470 480 490 500
DIDANGIVHV TAKDKGTGKE NTIRIQEGSG LSKEDIDRMI KDAEAHAEED
510 520 530 540 550
RKRREEADVR NQAETLVYQT EKFVKEQREA EGGSKVPEDT LNKVDAAVAE
560 570 580 590 600
AKAALGGSDI SAIKSAMEKL GQESQALGQA IYEAAQAASQ ATGAAHPGGE
610 620
PGGAHPGSAD DVVDAEVVDD GREAK
Length:625
Mass (Da):66,831
Last modified:April 16, 2014 - v1
Checksum:i23935906094D1A91
GO

Sequence cautioni

The sequence CAA41306 differs from that shown. Reason: Frameshift at position 552. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1542LA → PG in CAA41306 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58406 Genomic DNA. Translation: CAA41306.1. Frameshift.
JX026662 Genomic DNA. Translation: AFM37299.1.
AL123456 Genomic DNA. Translation: CCP43080.1.
PIRiG70574.
RefSeqiNP_214864.1. NC_000962.3.
WP_003401814.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP43080; CCP43080; Rv0350.
GeneIDi885946.
KEGGimtu:Rv0350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58406 Genomic DNA. Translation: CAA41306.1. Frameshift.
JX026662 Genomic DNA. Translation: AFM37299.1.
AL123456 Genomic DNA. Translation: CCP43080.1.
PIRiG70574.
RefSeqiNP_214864.1. NC_000962.3.
WP_003401814.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliP9WMJ9.
SMRiP9WMJ9. Positions 1-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP9WMJ9. 1 interaction.
STRINGi83332.Rv0350.

Proteomic databases

PaxDbiP9WMJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP43080; CCP43080; Rv0350.
GeneIDi885946.
KEGGimtu:Rv0350.

Organism-specific databases

TubercuListiRv0350.

Phylogenomic databases

eggNOGiENOG4105CFG. Bacteria.
COG0443. LUCA.
KOiK04043.
OMAiEKMAPPQ.
PhylomeDBiP9WMJ9.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK. 1 hit.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 2 hits.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAK_MYCTU
AccessioniPrimary (citable) accession number: P9WMJ9
Secondary accession number(s): I6QM76
, O06301, P0A5B9, P32723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: December 9, 2015
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.