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Protein

Transcriptional regulatory protein DevR (DosR)

Gene

devR

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system DevR/DevS (DosR/DosS) involved in onset of the dormancy response. When phosphorylated binds the promoter of at least its own and Acr (hspX) gene in response to hypoxia. Activates its own transcription under hypoxic but not aerobic conditions, probably binds as a dimer to tandem binding sites within the devR and hspX promoters. Accepts a phosphate group from DevS (DosS) and from DosT.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi167 – 186H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: MTBBASE
  • transcription factor activity, sequence-specific DNA binding Source: MTBBASE

GO - Biological processi

  • dormancy process Source: MTBBASE
  • pathogenesis Source: MTBBASE
  • phosphorelay signal transduction system Source: UniProtKB-KW
  • positive regulation of transcription, DNA-templated Source: MTBBASE
  • regulation of fatty acid metabolic process Source: MTBBASE
  • regulation of response to stress Source: MTBBASE
  • response to hypoxia Source: MTBBASE
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-7397-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein DevR (DosR)
Gene namesi
Name:devR
Synonyms:dosR
Ordered Locus Names:Rv3133c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3133c.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular space Source: MTBBASE
  • host cell cytoplasmic vesicle Source: UniProtKB-SubCell
  • host cell endosome lumen Source: MTBBASE
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Host cytoplasmic vesicle

Pathology & Biotechi

Disruption phenotypei

Strains deleted for this gene show hypervirulence upon intravenous innoculation in the mouse and DBA/2 model (PubMed:12595424), however contradictory results were shown for using a devR/devS deletion strain in rabbits, guinea pigs and C57BL/6 mice (PubMed:19103767). All studies agree that deletion strains fail to induce the dormancy regulon genes in response to hypoxia, NO, and CO.5 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8D → N: No phosphorylation by DevS (DosS). 1 Publication1
Mutagenesisi9D → N: No phosphorylation by DevS (DosS). 1 Publication1
Mutagenesisi54D → E: Able to bind DNA, no longer induces hypoxic response. 2 Publications1
Mutagenesisi54D → V: No phosphorylation by DevS (DosS) nor by DosT. 2 Publications1
Mutagenesisi104K → E: No phosphorylation by DevS (DosS). 1 Publication1
Mutagenesisi198T → A: Decreases phosphorylation by PknH. 1 Publication1
Mutagenesisi205T → A: No phosphorylation by PknH. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL6064.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003926251 – 217Transcriptional regulatory protein DevR (DosR)Add BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei544-aspartylphosphateCurated1
Modified residuei198Phosphothreonine; by PknH1 Publication1
Modified residuei205Phosphothreonine; by PknH1 Publication1

Post-translational modificationi

Phosphorylated on aspartate by both DevS (DosS) and DosT. Phosphorylated on threonine by PknH. Phosphorylation by PknH enhances DevR dimerization. Aspartate phosphorylation and threonine phosphorylation cooperatively enhance DevR binding to DNA.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP9WMF9.

PTM databases

iPTMnetiP9WMF9.

Expressioni

Inductioni

A member of the dormancy regulon. Moderately expressed under aerobic conditions, it is strongly induced in response to reduced oxygen tension (hypoxia), low levels of nitric oxide (NO) and carbon monoxide (CO). It is hoped that this regulon will give insight into the latent, or dormant phase of infection. Expression under hypoxic conditions, but not aerobically, is autoregulated. Member of the Rv3134c-devR-devS operon.5 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv3133c.

Structurei

Secondary structure

1217
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi11 – 22Combined sources12
Beta strandi27 – 35Combined sources9
Helixi36 – 46Combined sources11
Beta strandi49 – 53Combined sources5
Beta strandi55 – 57Combined sources3
Helixi62 – 72Combined sources11
Beta strandi77 – 80Combined sources4
Helixi81 – 83Combined sources3
Beta strandi84 – 86Combined sources3
Helixi87 – 96Combined sources10
Helixi101 – 121Combined sources21
Helixi122 – 124Combined sources3
Helixi127 – 143Combined sources17
Turni146 – 149Combined sources4
Helixi152 – 162Combined sources11
Helixi167 – 174Combined sources8
Helixi178 – 192Combined sources15
Helixi199 – 207Combined sources9
Turni208 – 210Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZLJX-ray2.00A/B/C/D/E/F/G/H144-217[»]
1ZLKX-ray3.10A/B144-217[»]
3C3WX-ray2.20A/B1-217[»]
3C57X-ray1.70A/B144-217[»]
ProteinModelPortaliP9WMF9.
SMRiP9WMF9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 119Response regulatoryPROSITE-ProRule annotationAdd BLAST117
Domaini143 – 208HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107NGZ. Bacteria.
COG2197. LUCA.
KOiK07695.
OMAiKGLRFFF.
PhylomeDBiP9WMF9.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WMF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVFLVDDH EVVRRGLVDL LGADPELDVV GEAGSVAEAM ARVPAARPDV
60 70 80 90 100
AVLDVRLPDG NGIELCRDLL SRMPDLRCLI LTSYTSDEAM LDAILAGASG
110 120 130 140 150
YVVKDIKGME LARAVKDVGA GRSLLDNRAA AALMAKLRGA AEKQDPLSGL
160 170 180 190 200
TDQERTLLGL LSEGLTNKQI ADRMFLAEKT VKNYVSRLLA KLGMERRTQA
210
AVFATELKRS RPPGDGP
Length:217
Mass (Da):23,294
Last modified:April 16, 2014 - v1
Checksum:i1BA535C26CC4EB51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22037 Genomic DNA. Translation: AAD17452.1.
AL123456 Genomic DNA. Translation: CCP45943.1.
PIRiF70645.
RefSeqiNP_217649.1. NC_000962.3.
WP_003416369.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP45943; CCP45943; Rv3133c.
GeneIDi888842.
KEGGimtu:Rv3133c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22037 Genomic DNA. Translation: AAD17452.1.
AL123456 Genomic DNA. Translation: CCP45943.1.
PIRiF70645.
RefSeqiNP_217649.1. NC_000962.3.
WP_003416369.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZLJX-ray2.00A/B/C/D/E/F/G/H144-217[»]
1ZLKX-ray3.10A/B144-217[»]
3C3WX-ray2.20A/B1-217[»]
3C57X-ray1.70A/B144-217[»]
ProteinModelPortaliP9WMF9.
SMRiP9WMF9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3133c.

Chemistry databases

ChEMBLiCHEMBL6064.

PTM databases

iPTMnetiP9WMF9.

Proteomic databases

PaxDbiP9WMF9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45943; CCP45943; Rv3133c.
GeneIDi888842.
KEGGimtu:Rv3133c.

Organism-specific databases

TubercuListiRv3133c.

Phylogenomic databases

eggNOGiENOG4107NGZ. Bacteria.
COG2197. LUCA.
KOiK07695.
OMAiKGLRFFF.
PhylomeDBiP9WMF9.

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-7397-MONOMER.

Miscellaneous databases

PROiP9WMF9.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEVR_MYCTU
AccessioniPrimary (citable) accession number: P9WMF9
Secondary accession number(s): L0TBX9
, P95193, Q79CX8, Q7D625
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.