Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P9WKK7

- ACEA_MYCTU

UniProt

P9WKK7 - ACEA_MYCTU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isocitrate lyase

Gene

icl

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway (By similarity).By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei191 – 1911

GO - Molecular functioni

  1. isocitrate lyase activity Source: MTBBASE
  2. methylisocitrate lyase activity Source: MTBBASE

GO - Biological processi

  1. cellular response to hypoxia Source: MTBBASE
  2. glyoxylate cycle Source: MTBBASE
  3. isocitrate metabolic process Source: MTBBASE
  4. maintenance of symbiont tolerance to host environment Source: MTBBASE
  5. pathogenesis Source: MTBBASE
  6. response to acetate Source: MTBBASE
  7. response to acid chemical Source: MTBBASE
  8. response to fatty acid Source: MTBBASE
  9. response to host immune response Source: MTBBASE
  10. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase (EC:4.1.3.1)
Short name:
ICL
Short name:
Isocitrase
Short name:
Isocitratase
Gene namesi
Name:icl
Ordered Locus Names:Rv0467
ORF Names:MTV038.11
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv0467.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: MTBBASE
  2. plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Isocitrate lyasePRO_0000068779Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki334 – 334Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Post-translational modificationi

Pupylated at Lys-334 by the prokaryotic ubiquitin-like protein Pup, which leads to its degradation by the proteasome.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

Secondary structure

1
428
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 1910
Helixi21 – 233
Helixi32 – 387
Helixi47 – 6216
Beta strandi66 – 705
Helixi74 – 829
Beta strandi88 – 903
Helixi92 – 987
Beta strandi108 – 1103
Helixi116 – 13823
Beta strandi150 – 1534
Turni155 – 1584
Helixi161 – 17313
Beta strandi177 – 1826
Helixi186 – 1883
Helixi202 – 21817
Beta strandi224 – 2296
Turni231 – 2333
Beta strandi236 – 2383
Turni243 – 2453
Helixi246 – 2483
Beta strandi249 – 2535
Beta strandi259 – 2613
Helixi265 – 27511
Helixi276 – 2783
Beta strandi280 – 2845
Helixi291 – 30212
Beta strandi309 – 3135
Beta strandi316 – 3183
Helixi320 – 3234
Helixi326 – 33813
Beta strandi341 – 3466
Helixi349 – 36820
Helixi370 – 38314
Helixi384 – 3863
Helixi393 – 3964
Helixi399 – 40911
Helixi422 – 4265

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F61X-ray2.00A/B2-428[»]
1F8IX-ray2.25A/B/C/D2-428[»]
1F8MX-ray1.80A/B/C/D2-428[»]
ProteinModelPortaliP9WKK7.
SMRiP9WKK7. Positions 2-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK01637.
PhylomeDBiP9WKK7.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WKK7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVVGTPKSA EQIQQEWDTN PRWKDVTRTY SAEDVVALQG SVVEEHTLAR
60 70 80 90 100
RGAEVLWEQL HDLEWVNALG ALTGNMAVQQ VRAGLKAIYL SGWQVAGDAN
110 120 130 140 150
LSGHTYPDQS LYPANSVPQV VRRINNALQR ADQIAKIEGD TSVENWLAPI
160 170 180 190 200
VADGEAGFGG ALNVYELQKA LIAAGVAGSH WEDQLASEKK CGHLGGKVLI
210 220 230 240 250
PTQQHIRTLT SARLAADVAD VPTVVIARTD AEAATLITSD VDERDQPFIT
260 270 280 290 300
GERTREGFYR TKNGIEPCIA RAKAYAPFAD LIWMETGTPD LEAARQFSEA
310 320 330 340 350
VKAEYPDQML AYNCSPSFNW KKHLDDATIA KFQKELAAMG FKFQFITLAG
360 370 380 390 400
FHALNYSMFD LAYGYAQNQM SAYVELQERE FAAEERGYTA TKHQREVGAG
410 420
YFDRIATTVD PNSSTTALTG STEEGQFH
Length:428
Mass (Da):47,087
Last modified:April 16, 2014 - v1
Checksum:iE5223F38CB5D9E8B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP43200.1.
PIRiG70828.
RefSeqiYP_006513796.1. NC_018143.2.
YP_177728.1. NC_000962.3.

Genome annotation databases

GeneIDi13318337.
886291.
KEGGimtu:Rv0467.
mtv:RVBD_0467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP43200.1 .
PIRi G70828.
RefSeqi YP_006513796.1. NC_018143.2.
YP_177728.1. NC_000962.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F61 X-ray 2.00 A/B 2-428 [» ]
1F8I X-ray 2.25 A/B/C/D 2-428 [» ]
1F8M X-ray 1.80 A/B/C/D 2-428 [» ]
ProteinModelPortali P9WKK7.
SMRi P9WKK7. Positions 2-427.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1667699.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13318337.
886291.
KEGGi mtu:Rv0467.
mtv:RVBD_0467.

Organism-specific databases

TubercuListi Rv0467.

Phylogenomic databases

KOi K01637.
PhylomeDBi P9WKK7.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00719 .

Family and domain databases

Gene3Di 3.20.20.60. 1 hit.
InterProi IPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
PANTHERi PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfami PF00463. ICL. 1 hit.
[Graphical view ]
PIRSFi PIRSF001362. Isocit_lyase. 1 hit.
SUPFAMi SSF51621. SSF51621. 1 hit.
TIGRFAMsi TIGR01346. isocit_lyase. 2 hits.
PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "Prokayrotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis."
    Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E., Gygi S.P., Darwin K.H.
    PLoS ONE 5:E8589-E8589(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEASOME SUBSTRATE, PUPYLATION AT LYS-334, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: ATCC 25618 / H37Rv.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  4. "Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis."
    Sharma V., Sharma S., zu Bentrup K.H., McKinney J.D., Russell D.G., Jacobs W.R. Jr., Sacchettini J.C.
    Nat. Struct. Biol. 7:663-668(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiACEA_MYCTU
AccessioniPrimary (citable) accession number: P9WKK7
Secondary accession number(s): L0T3N9, O53752, P0A5H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 29, 2014
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Was identified as a natural substrate of the M.tuberculosis proteasome.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3