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P9WKK7

- ACEA_MYCTU

UniProt

P9WKK7 - ACEA_MYCTU

Protein

Isocitrate lyase

Gene

icl

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 6 (01 Oct 2014)
      Sequence version 1 (16 Apr 2014)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway By similarity.By similarity

    Catalytic activityi

    Isocitrate = succinate + glyoxylate.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei191 – 1911

    GO - Molecular functioni

    1. isocitrate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-UniPathway
    2. response to host immune response Source: MTBBASE
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00719.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate lyase (EC:4.1.3.1)
    Short name:
    ICL
    Short name:
    Isocitrase
    Short name:
    Isocitratase
    Gene namesi
    Name:icl
    Ordered Locus Names:Rv0467
    ORF Names:MTV038.11
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001584: Chromosome

    Organism-specific databases

    TubercuListiRv0467.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: MTBBASE
    2. plasma membrane Source: MTBBASE

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 428428Isocitrate lyasePRO_0000068779Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki334 – 334Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

    Post-translational modificationi

    Pupylated at Lys-334 by the prokaryotic ubiquitin-like protein Pup, which leads to its degradation by the proteasome.1 Publication

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    Secondary structure

    1
    428
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 1910
    Helixi21 – 233
    Helixi32 – 387
    Helixi47 – 6216
    Beta strandi66 – 705
    Helixi74 – 829
    Beta strandi88 – 903
    Helixi92 – 987
    Beta strandi108 – 1103
    Helixi116 – 13823
    Beta strandi150 – 1534
    Turni155 – 1584
    Helixi161 – 17313
    Beta strandi177 – 1826
    Helixi186 – 1883
    Helixi202 – 21817
    Beta strandi224 – 2296
    Turni231 – 2333
    Beta strandi236 – 2383
    Turni243 – 2453
    Helixi246 – 2483
    Beta strandi249 – 2535
    Beta strandi259 – 2613
    Helixi265 – 27511
    Helixi276 – 2783
    Beta strandi280 – 2845
    Helixi291 – 30212
    Beta strandi309 – 3135
    Beta strandi316 – 3183
    Helixi320 – 3234
    Helixi326 – 33813
    Beta strandi341 – 3466
    Helixi349 – 36820
    Helixi370 – 38314
    Helixi384 – 3863
    Helixi393 – 3964
    Helixi399 – 40911
    Helixi422 – 4265

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1F61X-ray2.00A/B2-428[»]
    1F8IX-ray2.25A/B/C/D2-428[»]
    1F8MX-ray1.80A/B/C/D2-428[»]
    ProteinModelPortaliP9WKK7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    KOiK01637.
    PhylomeDBiP9WKK7.

    Family and domain databases

    Gene3Di3.20.20.60. 1 hit.
    InterProiIPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
    PfamiPF00463. ICL. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001362. Isocit_lyase. 1 hit.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    TIGRFAMsiTIGR01346. isocit_lyase. 2 hits.
    PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P9WKK7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVVGTPKSA EQIQQEWDTN PRWKDVTRTY SAEDVVALQG SVVEEHTLAR    50
    RGAEVLWEQL HDLEWVNALG ALTGNMAVQQ VRAGLKAIYL SGWQVAGDAN 100
    LSGHTYPDQS LYPANSVPQV VRRINNALQR ADQIAKIEGD TSVENWLAPI 150
    VADGEAGFGG ALNVYELQKA LIAAGVAGSH WEDQLASEKK CGHLGGKVLI 200
    PTQQHIRTLT SARLAADVAD VPTVVIARTD AEAATLITSD VDERDQPFIT 250
    GERTREGFYR TKNGIEPCIA RAKAYAPFAD LIWMETGTPD LEAARQFSEA 300
    VKAEYPDQML AYNCSPSFNW KKHLDDATIA KFQKELAAMG FKFQFITLAG 350
    FHALNYSMFD LAYGYAQNQM SAYVELQERE FAAEERGYTA TKHQREVGAG 400
    YFDRIATTVD PNSSTTALTG STEEGQFH 428
    Length:428
    Mass (Da):47,087
    Last modified:April 16, 2014 - v1
    Checksum:iE5223F38CB5D9E8B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL123456 Genomic DNA. Translation: CCP43200.1.
    PIRiG70828.
    RefSeqiYP_006513796.1. NC_018143.2.
    YP_177728.1. NC_000962.3.

    Genome annotation databases

    GeneIDi13318337.
    886291.
    KEGGimtu:Rv0467.
    mtv:RVBD_0467.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL123456 Genomic DNA. Translation: CCP43200.1 .
    PIRi G70828.
    RefSeqi YP_006513796.1. NC_018143.2.
    YP_177728.1. NC_000962.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1F61 X-ray 2.00 A/B 2-428 [» ]
    1F8I X-ray 2.25 A/B/C/D 2-428 [» ]
    1F8M X-ray 1.80 A/B/C/D 2-428 [» ]
    ProteinModelPortali P9WKK7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13318337.
    886291.
    KEGGi mtu:Rv0467.
    mtv:RVBD_0467.

    Organism-specific databases

    TubercuListi Rv0467.

    Phylogenomic databases

    KOi K01637.
    PhylomeDBi P9WKK7.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00719 .

    Family and domain databases

    Gene3Di 3.20.20.60. 1 hit.
    InterProi IPR006254. Isocitrate_lyase.
    IPR018523. Isocitrate_lyase_ph_CS.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    PANTHERi PTHR21631:SF3. PTHR21631:SF3. 1 hit.
    Pfami PF00463. ICL. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001362. Isocit_lyase. 1 hit.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    TIGRFAMsi TIGR01346. isocit_lyase. 2 hits.
    PROSITEi PS00161. ISOCITRATE_LYASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    2. "Prokayrotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis."
      Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E., Gygi S.P., Darwin K.H.
      PLoS ONE 5:E8589-E8589(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEASOME SUBSTRATE, PUPYLATION AT LYS-334, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: ATCC 25618 / H37Rv.
    3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 25618 / H37Rv.
    4. "Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis."
      Sharma V., Sharma S., zu Bentrup K.H., McKinney J.D., Russell D.G., Jacobs W.R. Jr., Sacchettini J.C.
      Nat. Struct. Biol. 7:663-668(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

    Entry informationi

    Entry nameiACEA_MYCTU
    AccessioniPrimary (citable) accession number: P9WKK7
    Secondary accession number(s): L0T3N9, O53752, P0A5H3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 6 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Was identified as a natural substrate of the M.tuberculosis proteasome.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3