Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Inositol-3-phosphate synthase

Gene

ino1

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of glucose 6-phosphate to 1D-myo-inositol 3-phosphate.By similarity

Catalytic activityi

D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate.

GO - Molecular functioni

  • inositol-3-phosphate synthase activity Source: MTBBASE
  • zinc ion binding Source: MTBBASE

GO - Biological processi

  • inositol biosynthetic process Source: UniProtKB-KW
  • mycothiol biosynthetic process Source: Reactome
  • pathogenesis Source: MTBBASE
  • phospholipid biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Inositol biosynthesis

Enzyme and pathway databases

ReactomeiR-MTU-879299. Mycothiol biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-3-phosphate synthase (EC:5.5.1.4)
Short name:
IPS
Alternative name(s):
Myo-inositol 1-phosphate synthase
Short name:
MI-1-P synthase
Short name:
MIP synthase
Gene namesi
Name:ino1
Ordered Locus Names:Rv0046c
ORF Names:MTCY21D4.09c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0046c.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 367366Inositol-3-phosphate synthasePRO_0000195203Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Cross-linki73 – 73Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Post-translational modificationi

Pupylated at Lys-73 by the prokaryotic ubiquitin-like protein Pup, which leads to its degradation by the proteasome.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP9WKI1.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0046c.

Structurei

Secondary structure

1
367
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 216Combined sources
Helixi25 – 3612Combined sources
Turni37 – 393Combined sources
Beta strandi52 – 543Combined sources
Helixi59 – 613Combined sources
Beta strandi62 – 698Combined sources
Turni72 – 765Combined sources
Helixi79 – 824Combined sources
Helixi114 – 1174Combined sources
Helixi130 – 1367Combined sources
Beta strandi140 – 1445Combined sources
Helixi151 – 16414Combined sources
Beta strandi167 – 1704Combined sources
Helixi180 – 18910Combined sources
Beta strandi192 – 1987Combined sources
Helixi204 – 21714Combined sources
Beta strandi221 – 23111Combined sources
Helixi234 – 2407Combined sources
Beta strandi272 – 2765Combined sources
Helixi278 – 2803Combined sources
Beta strandi283 – 29412Combined sources
Helixi295 – 2973Combined sources
Beta strandi298 – 30912Combined sources
Helixi312 – 33019Combined sources
Helixi338 – 3447Combined sources
Beta strandi345 – 3473Combined sources
Helixi354 – 36613Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GR0X-ray1.95A1-367[»]
ProteinModelPortaliP9WKI1.
SMRiP9WKI1. Positions 14-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CZA. Bacteria.
COG1260. LUCA.
KOiK01858.
OMAiLVCYLPV.
PhylomeDBiP9WKI1.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002587. Myo-inos-1-P_Synthase.
IPR017815. Myo-inos-1-P_Synthase_actino.
IPR013021. Myo-inos-1-P_Synthase_GAPDH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11510. PTHR11510. 1 hit.
PfamiPF01658. Inos-1-P_synth. 1 hit.
[Graphical view]
PIRSFiPIRSF015578. Myoinos-ppht_syn. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR03450. mycothiol_INO1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WKI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEHQSLPAP EASTEVRVAI VGVGNCASSL VQGVEYYYNA DDTSTVPGLM
60 70 80 90 100
HVRFGPYHVR DVKFVAAFDV DAKKVGFDLS DAIFASENNT IKIADVAPTN
110 120 130 140 150
VIVQRGPTLD GIGKYYADTI ELSDAEPVDV VQALKEAKVD VLVSYLPVGS
160 170 180 190 200
EEADKFYAQC AIDAGVAFVN ALPVFIASDP VWAKKFTDAR VPIVGDDIKS
210 220 230 240 250
QVGATITHRV LAKLFEDRGV QLDRTMQLNV GGNMDFLNML ERERLESKKI
260 270 280 290 300
SKTQAVTSNL KREFKTKDVH IGPSDHVGWL DDRKWAYVRL EGRAFGDVPL
310 320 330 340 350
NLEYKLEVWD SPNSAGVIID AVRAAKIAKD RGIGGPVIPA SAYLMKSPPE
360
QLPDDIARAQ LEEFIIG
Length:367
Mass (Da):40,094
Last modified:April 16, 2014 - v1
Checksum:i3976AA08452183F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP42768.1.
PIRiF70912.
RefSeqiNP_214560.1. NC_000962.3.
WP_003902822.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP42768; CCP42768; Rv0046c.
GeneIDi887028.
KEGGimtu:Rv0046c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP42768.1.
PIRiF70912.
RefSeqiNP_214560.1. NC_000962.3.
WP_003902822.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GR0X-ray1.95A1-367[»]
ProteinModelPortaliP9WKI1.
SMRiP9WKI1. Positions 14-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv0046c.

Proteomic databases

PaxDbiP9WKI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP42768; CCP42768; Rv0046c.
GeneIDi887028.
KEGGimtu:Rv0046c.

Organism-specific databases

TubercuListiRv0046c.

Phylogenomic databases

eggNOGiENOG4105CZA. Bacteria.
COG1260. LUCA.
KOiK01858.
OMAiLVCYLPV.
PhylomeDBiP9WKI1.

Enzyme and pathway databases

ReactomeiR-MTU-879299. Mycothiol biosynthesis.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002587. Myo-inos-1-P_Synthase.
IPR017815. Myo-inos-1-P_Synthase_actino.
IPR013021. Myo-inos-1-P_Synthase_GAPDH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11510. PTHR11510. 1 hit.
PfamiPF01658. Inos-1-P_synth. 1 hit.
[Graphical view]
PIRSFiPIRSF015578. Myoinos-ppht_syn. 1 hit.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR03450. mycothiol_INO1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiINO1_MYCTU
AccessioniPrimary (citable) accession number: P9WKI1
Secondary accession number(s): L0T457, P71703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: June 8, 2016
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Was identified as a natural substrate of the M.tuberculosis proteasome.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.