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Protein

Inositol-3-phosphate synthase

Gene

ino1

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of glucose 6-phosphate to 1D-myo-inositol 3-phosphate.By similarity

Miscellaneous

Was identified as a natural substrate of the M.tuberculosis proteasome.

Catalytic activityi

D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate.

GO - Molecular functioni

  • inositol-3-phosphate synthase activity Source: MTBBASE
  • zinc ion binding Source: MTBBASE

GO - Biological processi

  • inositol biosynthetic process Source: UniProtKB-KW
  • mycothiol biosynthetic process Source: Reactome
  • pathogenesis Source: MTBBASE
  • phospholipid biosynthetic process Source: InterPro

Keywordsi

Molecular functionIsomerase
Biological processInositol biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:G185E-4160-MONOMER
ReactomeiR-MTU-879299 Mycothiol biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-3-phosphate synthase (EC:5.5.1.4)
Short name:
IPS
Alternative name(s):
Myo-inositol 1-phosphate synthase
Short name:
MI-1-P synthase
Short name:
MIP synthase
Gene namesi
Name:ino1
Ordered Locus Names:Rv0046c
ORF Names:MTCY21D4.09c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi

Organism-specific databases

TubercuListiRv0046c

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001952032 – 367Inositol-3-phosphate synthaseAdd BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Cross-linki73Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Post-translational modificationi

Pupylated at Lys-73 by the prokaryotic ubiquitin-like protein Pup, which leads to its degradation by the proteasome.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP9WKI1

PTM databases

iPTMnetiP9WKI1

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0046c

Structurei

Secondary structure

1367
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 21Combined sources6
Helixi25 – 36Combined sources12
Turni37 – 39Combined sources3
Beta strandi52 – 54Combined sources3
Helixi59 – 61Combined sources3
Beta strandi62 – 69Combined sources8
Turni72 – 76Combined sources5
Helixi79 – 82Combined sources4
Helixi114 – 117Combined sources4
Helixi130 – 136Combined sources7
Beta strandi140 – 144Combined sources5
Helixi151 – 164Combined sources14
Beta strandi167 – 170Combined sources4
Helixi180 – 189Combined sources10
Beta strandi192 – 198Combined sources7
Helixi204 – 217Combined sources14
Beta strandi221 – 231Combined sources11
Helixi234 – 240Combined sources7
Beta strandi272 – 276Combined sources5
Helixi278 – 280Combined sources3
Beta strandi283 – 294Combined sources12
Helixi295 – 297Combined sources3
Beta strandi298 – 309Combined sources12
Helixi312 – 330Combined sources19
Helixi338 – 344Combined sources7
Beta strandi345 – 347Combined sources3
Helixi354 – 366Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GR0X-ray1.95A1-367[»]
ProteinModelPortaliP9WKI1
SMRiP9WKI1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CZA Bacteria
COG1260 LUCA
KOiK01858
OMAiCFLRMEG
PhylomeDBiP9WKI1

Family and domain databases

InterProiView protein in InterPro
IPR002587 Myo-inos-1-P_Synthase
IPR017815 Myo-inos-1-P_Synthase_actino
IPR013021 Myo-inos-1-P_Synthase_GAPDH
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01658 Inos-1-P_synth, 1 hit
PIRSFiPIRSF015578 Myoinos-ppht_syn, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits
TIGRFAMsiTIGR03450 mycothiol_INO1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WKI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEHQSLPAP EASTEVRVAI VGVGNCASSL VQGVEYYYNA DDTSTVPGLM
60 70 80 90 100
HVRFGPYHVR DVKFVAAFDV DAKKVGFDLS DAIFASENNT IKIADVAPTN
110 120 130 140 150
VIVQRGPTLD GIGKYYADTI ELSDAEPVDV VQALKEAKVD VLVSYLPVGS
160 170 180 190 200
EEADKFYAQC AIDAGVAFVN ALPVFIASDP VWAKKFTDAR VPIVGDDIKS
210 220 230 240 250
QVGATITHRV LAKLFEDRGV QLDRTMQLNV GGNMDFLNML ERERLESKKI
260 270 280 290 300
SKTQAVTSNL KREFKTKDVH IGPSDHVGWL DDRKWAYVRL EGRAFGDVPL
310 320 330 340 350
NLEYKLEVWD SPNSAGVIID AVRAAKIAKD RGIGGPVIPA SAYLMKSPPE
360
QLPDDIARAQ LEEFIIG
Length:367
Mass (Da):40,094
Last modified:April 16, 2014 - v1
Checksum:i3976AA08452183F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP42768.1
PIRiF70912
RefSeqiNP_214560.1, NC_000962.3
WP_003902822.1, NZ_KK339370.1

Genome annotation databases

EnsemblBacteriaiCCP42768; CCP42768; Rv0046c
GeneIDi887028
KEGGimtu:Rv0046c

Similar proteinsi

Entry informationi

Entry nameiINO1_MYCTU
AccessioniPrimary (citable) accession number: P9WKI1
Secondary accession number(s): L0T457, P71703
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: March 28, 2018
This is version 28 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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