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Protein

Elongation factor 4

Gene

lepA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 676GTPUniRule annotation
Nucleotide bindingi178 – 1814GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:Rv2404c
ORF Names:MTCY253.16
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2404c.

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 653653Elongation factor 4PRO_0000176307Add
BLAST

Proteomic databases

PaxDbiP9WK97.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv2404c.

Structurei

3D structure databases

ProteinModelPortaliP9WK97.
SMRiP9WK97. Positions 52-596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 231182tr-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
KOiK03596.
OMAiKPMVFCG.
PhylomeDBiP9WK97.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WK97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTPCSQHRR DRPSAIGSQL PDADTLDTRQ PPLQEIPISS FADKTFTAPA
60 70 80 90 100
QIRNFCIIAH IDHGKSTLAD RMLQLTGVVD ERSMRAQYLD RMDIERERGI
110 120 130 140 150
TIKAQNVRLP WRVDKTDYVL HLIDTPGHVD FTYEVSRALE ACEGAVLLVD
160 170 180 190 200
AAQGIEAQTL ANLYLALDRD LHIIPVLNKI DLPAADPDRY AAEMAHIIGC
210 220 230 240 250
EPAEVLRVSG KTGEGVSDLL DEVVRQVPPP QGDAEAPTRA MIFDSVYDIY
260 270 280 290 300
RGVVTYVRVV DGKISPRERI MMMSTGATHE LLEVGIVSPE PKPCEGLGVG
310 320 330 340 350
EVGYLITGVK DVRQSKVGDT VTSLSRARGA AAEALTGYRE PKPMVYSGLY
360 370 380 390 400
PVDGSDYPNL RDALDKLQLN DAALTYEPET SVALGFGFRC GFLGLLHMEI
410 420 430 440 450
TRERLEREFG LDLISTSPNV VYRVHKDDGT EIRVTNPSDW PEGKIRTVYE
460 470 480 490 500
PVVKTTIIAP SEFIGTIMEL CQSRRGELGG MDYLSPERVE LRYTMPLGEI
510 520 530 540 550
IFDFFDALKS RTRGYASLDY EEAGEQEAAL VKVDILLQGE AVDAFSAIVH
560 570 580 590 600
KDTAYAYGNK MTTKLKELIP RQQFEVPVQA AIGSKIIARE NIRAIRKDVL
610 620 630 640 650
SKCYGGDITR KRKLLEKQKE GKKRMKTIGR VEVPQEAFVA ALSTDAAGDK

GKK
Length:653
Mass (Da):72,396
Last modified:April 16, 2014 - v1
Checksum:iDA4AFE10E6C25755
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45195.1.
PIRiG70683.
RefSeqiNP_216920.1. NC_000962.3.
WP_003900516.1. NC_000962.3.

Genome annotation databases

EnsemblBacteriaiCCP45195; CCP45195; Rv2404c.
GeneIDi885475.
KEGGimtu:Rv2404c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45195.1.
PIRiG70683.
RefSeqiNP_216920.1. NC_000962.3.
WP_003900516.1. NC_000962.3.

3D structure databases

ProteinModelPortaliP9WK97.
SMRiP9WK97. Positions 52-596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2404c.

Proteomic databases

PaxDbiP9WK97.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45195; CCP45195; Rv2404c.
GeneIDi885475.
KEGGimtu:Rv2404c.

Organism-specific databases

TubercuListiRv2404c.

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
KOiK03596.
OMAiKPMVFCG.
PhylomeDBiP9WK97.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "Cloning and characterization of GTP-binding proteins of Mycobacterium tuberculosis H37Rv."
    Meena L.S., Chopra P., Bedwal R.S., Singh Y.
    Enzyme Microb. Technol. 42:138-144(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A GTPASE, GTP-BINDING.
    Strain: H37Rv.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiLEPA_MYCTU
AccessioniPrimary (citable) accession number: P9WK97
Secondary accession number(s): L0T9Q5, P65269, P71739
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 17, 2016
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.