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Protein

Elongation factor 4

Gene

lepA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 67GTPUniRule annotation6
Nucleotide bindingi178 – 181GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:Rv2404c
ORF Names:MTCY253.16
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2404c

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001763071 – 653Elongation factor 4Add BLAST653

Proteomic databases

PaxDbiP9WK97
PRIDEiP9WK97

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv2404c

Structurei

3D structure databases

ProteinModelPortaliP9WK97
SMRiP9WK97
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 231tr-type GAdd BLAST182

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S Bacteria
COG0481 LUCA
KOiK03596
OMAiALIFDSW
PhylomeDBiP9WK97

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR031157 G_TR_CS
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P9WK97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTPCSQHRR DRPSAIGSQL PDADTLDTRQ PPLQEIPISS FADKTFTAPA
60 70 80 90 100
QIRNFCIIAH IDHGKSTLAD RMLQLTGVVD ERSMRAQYLD RMDIERERGI
110 120 130 140 150
TIKAQNVRLP WRVDKTDYVL HLIDTPGHVD FTYEVSRALE ACEGAVLLVD
160 170 180 190 200
AAQGIEAQTL ANLYLALDRD LHIIPVLNKI DLPAADPDRY AAEMAHIIGC
210 220 230 240 250
EPAEVLRVSG KTGEGVSDLL DEVVRQVPPP QGDAEAPTRA MIFDSVYDIY
260 270 280 290 300
RGVVTYVRVV DGKISPRERI MMMSTGATHE LLEVGIVSPE PKPCEGLGVG
310 320 330 340 350
EVGYLITGVK DVRQSKVGDT VTSLSRARGA AAEALTGYRE PKPMVYSGLY
360 370 380 390 400
PVDGSDYPNL RDALDKLQLN DAALTYEPET SVALGFGFRC GFLGLLHMEI
410 420 430 440 450
TRERLEREFG LDLISTSPNV VYRVHKDDGT EIRVTNPSDW PEGKIRTVYE
460 470 480 490 500
PVVKTTIIAP SEFIGTIMEL CQSRRGELGG MDYLSPERVE LRYTMPLGEI
510 520 530 540 550
IFDFFDALKS RTRGYASLDY EEAGEQEAAL VKVDILLQGE AVDAFSAIVH
560 570 580 590 600
KDTAYAYGNK MTTKLKELIP RQQFEVPVQA AIGSKIIARE NIRAIRKDVL
610 620 630 640 650
SKCYGGDITR KRKLLEKQKE GKKRMKTIGR VEVPQEAFVA ALSTDAAGDK

GKK
Length:653
Mass (Da):72,396
Last modified:April 16, 2014 - v1
Checksum:iDA4AFE10E6C25755
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP45195.1
PIRiG70683
RefSeqiNP_216920.1, NC_000962.3
WP_003900516.1, NZ_KK339370.1

Genome annotation databases

EnsemblBacteriaiCCP45195; CCP45195; Rv2404c
GeneIDi885475
KEGGimtu:Rv2404c

Similar proteinsi

Entry informationi

Entry nameiLEPA_MYCTU
AccessioniPrimary (citable) accession number: P9WK97
Secondary accession number(s): L0T9Q5, P65269, P71739
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: May 23, 2018
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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