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Protein

Lipoprotein LprA

Gene

lprA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Constitutes a host TLR2 agonist (toll-like receptor), shown experimentally for human and mouse (PubMed:19362712). In host cells full-length (acylated) protein acts as a TLR2 agonist, inducing human and murine macrophages to produce cytokines, inducing murine dendritic cell maturation and cytokine production and inhibiting antibody processing in murine macrophages (PubMed:16785538). Binds diacylated phosphatidyl-myo-inositol mannosides (PIMs) (PubMed:20694006). Does not induce murine macrophage apoptosis or necrosis (PubMed:16785538). Non-acylated protein does not act as a TLR2 agonist (PubMed:20694006). Requires only host TLR2 as receptors to elicit host response in mouse, although TLR6 may play a redundant role, also requires CD14 and CD16 as accessory receptors (PubMed:19362712).3 Publications

GO - Molecular functioni

  • lipid binding Source: UniProtKB-KW
  • receptor agonist activity Source: MTBBASE

GO - Biological processi

Keywordsi

LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein LprA
Gene namesi
Name:lprA
Ordered Locus Names:Rv1270c
ORF Names:MTCY50.12
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1270c.

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Lipid-anchor PROSITE-ProRule annotation
  • Secretedextracellular space Curated

  • Note: May be shed from the cell surface in infected host cells.1 Publication

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • extracellular region Source: MTBBASE
  • extracellular space Source: UniProtKB-SubCell
  • plasma membrane Source: MTBBASE

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi25C → M: When expressed from this site no longer acts as a TLR2 agonist, suggests active protein is acylated. No glycosylated form detected. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24PROSITE-ProRule annotationAdd BLAST24
ChainiPRO_000001813525 – 244Lipoprotein LprAAdd BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi25N-palmitoyl cysteinePROSITE-ProRule annotation1 Publication1
Lipidationi25S-diacylglycerol cysteinePROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Modified by Lgt on Cys-25 with an S-linked diacylglyceral, signal peptide is removed by LspA, Cys-25 is further modifed with an amide group on the amino group by Lnt yielding a triacylated protein (PubMed:20694006). Upon expression in M.smegmatis non-glycosylated form and glycosylated forms are detected (by concanavalin A binding); when only the mature sequence is expressed from mutated protein only the non-glycosylated form is detected (PubMed:16785538).By similarity1 Publication1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP9WK55.

Interactioni

GO - Molecular functioni

  • receptor agonist activity Source: MTBBASE

Protein-protein interaction databases

STRINGi83332.Rv1270c.

Structurei

3D structure databases

ProteinModelPortaliP9WK55.
SMRiP9WK55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LppX/LprAFG lipoprotein family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107H5C. Bacteria.
ENOG410ZKNH. LUCA.
KOiK14955.
OMAiMHVNLTV.
PhylomeDBiP9WK55.

Family and domain databases

InterProiView protein in InterPro
IPR029046. LolA/LolB/LppX.
IPR009830. LppX/LprAFG.
PfamiView protein in Pfam
PF07161. LppX_LprAFG. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD017293. DUF1396. 1 hit.
SUPFAMiSSF89392. SSF89392. 1 hit.
PROSITEiView protein in PROSITE
PS51257. PROKAR_LIPOPROTEIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WK55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHPPCSVVA AATAILAVVL AIGGCSTEGD AGKASDTAAT ASNGDAAMLL
60 70 80 90 100
KQATDAMRKV TGMHVRLAVT GDVPNLRVTK LEGDISNTPQ TVATGSATLL
110 120 130 140 150
VGNKSEDAKF VYVDGHLYSD LGQPGTYTDF GNGASIYNVS VLLDPNKGLA
160 170 180 190 200
NLLANLKDAS VAGSQQADGV ATTKITGNSS ADDIATLAGS RLTSEDVKTV
210 220 230 240
PTTVWIASDG SSHLVQIQIA PTKDTSVTLT MSDWGKQVTA TKPV
Length:244
Mass (Da):24,874
Last modified:April 16, 2014 - v1
Checksum:i566C3A1459622BD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44026.1.
PIRiF70754.
RefSeqiNP_215786.1. NC_000962.3.
WP_003406562.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44026; CCP44026; Rv1270c.
GeneIDi887017.
KEGGimtu:Rv1270c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44026.1.
PIRiF70754.
RefSeqiNP_215786.1. NC_000962.3.
WP_003406562.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliP9WK55.
SMRiP9WK55.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1270c.

Proteomic databases

PaxDbiP9WK55.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP44026; CCP44026; Rv1270c.
GeneIDi887017.
KEGGimtu:Rv1270c.

Organism-specific databases

TubercuListiRv1270c.

Phylogenomic databases

eggNOGiENOG4107H5C. Bacteria.
ENOG410ZKNH. LUCA.
KOiK14955.
OMAiMHVNLTV.
PhylomeDBiP9WK55.

Family and domain databases

InterProiView protein in InterPro
IPR029046. LolA/LolB/LppX.
IPR009830. LppX/LprAFG.
PfamiView protein in Pfam
PF07161. LppX_LprAFG. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD017293. DUF1396. 1 hit.
SUPFAMiSSF89392. SSF89392. 1 hit.
PROSITEiView protein in PROSITE
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPRA_MYCTU
AccessioniPrimary (citable) accession number: P9WK55
Secondary accession number(s): L0T656, Q11049
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 15, 2017
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.