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Protein

Malate dehydrogenase

Gene

mdh

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93SubstrateUniRule annotation1
Binding sitei99SubstrateUniRule annotation1
Binding sitei106NADUniRule annotation1
Binding sitei113NADUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Binding sitei163SubstrateUniRule annotation1
Active sitei188Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADUniRule annotation7
Nucleotide bindingi130 – 132NADUniRule annotation3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: MTBBASE

GO - Biological processi

  • carbohydrate metabolic process Source: MTBBASE
  • malate metabolic process Source: GO_Central
  • NADH metabolic process Source: GO_Central
  • oxaloacetate metabolic process Source: GO_Central
  • tricarboxylic acid cycle Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:G185E-5411-MONOMER.
MTBH37RV:G185E-5411-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Rv1240
ORF Names:MTV006.12
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1240.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001133801 – 329Malate dehydrogenaseAdd BLAST329

Proteomic databases

PaxDbiP9WK13.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv1240.

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Helixi16 – 27Combined sources12
Turni28 – 31Combined sources4
Beta strandi37 – 42Combined sources6
Helixi45 – 47Combined sources3
Helixi48 – 59Combined sources12
Turni60 – 62Combined sources3
Beta strandi66 – 73Combined sources8
Helixi75 – 78Combined sources4
Turni79 – 81Combined sources3
Beta strandi83 – 87Combined sources5
Helixi99 – 102Combined sources4
Helixi103 – 120Combined sources18
Beta strandi126 – 129Combined sources4
Beta strandi131 – 133Combined sources3
Helixi134 – 143Combined sources10
Helixi150 – 152Combined sources3
Beta strandi153 – 155Combined sources3
Helixi158 – 172Combined sources15
Helixi176 – 178Combined sources3
Beta strandi183 – 186Combined sources4
Beta strandi193 – 195Combined sources3
Beta strandi200 – 205Combined sources6
Helixi206 – 210Combined sources5
Helixi213 – 218Combined sources6
Helixi220 – 225Combined sources6
Helixi227 – 235Combined sources9
Helixi241 – 256Combined sources16
Beta strandi264 – 269Combined sources6
Helixi273 – 275Combined sources3
Beta strandi281 – 289Combined sources9
Beta strandi292 – 295Combined sources4
Helixi303 – 325Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TVOX-ray1.50A/B2-329[»]
5KVVX-ray2.01A/B2-329[»]
ProteinModelPortaliP9WK13.
SMRiP9WK13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
KOiK00024.
OMAiAFSQECI.
PhylomeDBiP9WK13.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WK13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASPLKVAV TGAAGQIGYS LLFRLASGSL LGPDRPIELR LLEIEPALQA
60 70 80 90 100
LEGVVMELDD CAFPLLSGVE IGSDPQKIFD GVSLALLVGA RPRGAGMERS
110 120 130 140 150
DLLEANGAIF TAQGKALNAV AADDVRVGVT GNPANTNALI AMTNAPDIPR
160 170 180 190 200
ERFSALTRLD HNRAISQLAA KTGAAVTDIK KMTIWGNHSA TQYPDLFHAE
210 220 230 240 250
VAGKNAAEVV NDQAWIEDEF IPTVAKRGAA IIDARGASSA ASAASATIDA
260 270 280 290 300
ARDWLLGTPA DDWVSMAVVS DGSYGVPEGL ISSFPVTTKG GNWTIVSGLE
310 320
IDEFSRGRID KSTAELADER SAVTELGLI
Length:329
Mass (Da):34,322
Last modified:April 16, 2014 - v1
Checksum:i0A512659617F9C37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43996.1.
PIRiG70952.
RefSeqiNP_215756.1. NC_000962.3.
WP_003406301.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP43996; CCP43996; Rv1240.
GeneIDi887119.
KEGGimtu:Rv1240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43996.1.
PIRiG70952.
RefSeqiNP_215756.1. NC_000962.3.
WP_003406301.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TVOX-ray1.50A/B2-329[»]
5KVVX-ray2.01A/B2-329[»]
ProteinModelPortaliP9WK13.
SMRiP9WK13.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1240.

Proteomic databases

PaxDbiP9WK13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP43996; CCP43996; Rv1240.
GeneIDi887119.
KEGGimtu:Rv1240.

Organism-specific databases

TubercuListiRv1240.

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
KOiK00024.
OMAiAFSQECI.
PhylomeDBiP9WK13.

Enzyme and pathway databases

BioCyciMetaCyc:G185E-5411-MONOMER.
MTBH37RV:G185E-5411-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_MYCTU
AccessioniPrimary (citable) accession number: P9WK13
Secondary accession number(s): L0T626, O54592, P0A5J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 30, 2016
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.