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Protein

Malate dehydrogenase

Gene

mdh

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstrateUniRule annotation
Binding sitei99 – 991SubstrateUniRule annotation
Binding sitei106 – 1061NADUniRule annotation
Binding sitei113 – 1131NADUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Binding sitei163 – 1631SubstrateUniRule annotation
Active sitei188 – 1881Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NADUniRule annotation
Nucleotide bindingi130 – 1323NADUniRule annotation

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: MTBBASE

GO - Biological processi

  • carbohydrate metabolic process Source: MTBBASE
  • malate metabolic process Source: InterPro
  • tricarboxylic acid cycle Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Rv1240
ORF Names:MTV006.12
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1240.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Malate dehydrogenasePRO_0000113380Add
BLAST

Proteomic databases

PaxDbiP9WK13.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv1240.

Structurei

Secondary structure

1
329
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 127Combined sources
Helixi16 – 2712Combined sources
Turni28 – 314Combined sources
Beta strandi37 – 426Combined sources
Helixi45 – 473Combined sources
Helixi48 – 5912Combined sources
Turni60 – 623Combined sources
Beta strandi66 – 738Combined sources
Helixi75 – 784Combined sources
Turni79 – 813Combined sources
Beta strandi83 – 875Combined sources
Helixi99 – 1024Combined sources
Helixi103 – 12018Combined sources
Beta strandi126 – 1294Combined sources
Beta strandi131 – 1333Combined sources
Helixi134 – 14310Combined sources
Helixi150 – 1523Combined sources
Beta strandi153 – 1553Combined sources
Helixi158 – 17215Combined sources
Helixi176 – 1783Combined sources
Beta strandi183 – 1864Combined sources
Beta strandi193 – 1953Combined sources
Helixi206 – 2105Combined sources
Helixi213 – 2186Combined sources
Helixi220 – 2256Combined sources
Helixi227 – 2359Combined sources
Helixi241 – 25616Combined sources
Beta strandi264 – 2696Combined sources
Helixi273 – 2753Combined sources
Beta strandi281 – 2899Combined sources
Beta strandi292 – 2954Combined sources
Helixi303 – 32523Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4TVOX-ray1.50A/B2-329[»]
ProteinModelPortaliP9WK13.
SMRiP9WK13. Positions 3-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
KOiK00024.
OMAiAFSQECI.
PhylomeDBiP9WK13.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WK13-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASPLKVAV TGAAGQIGYS LLFRLASGSL LGPDRPIELR LLEIEPALQA
60 70 80 90 100
LEGVVMELDD CAFPLLSGVE IGSDPQKIFD GVSLALLVGA RPRGAGMERS
110 120 130 140 150
DLLEANGAIF TAQGKALNAV AADDVRVGVT GNPANTNALI AMTNAPDIPR
160 170 180 190 200
ERFSALTRLD HNRAISQLAA KTGAAVTDIK KMTIWGNHSA TQYPDLFHAE
210 220 230 240 250
VAGKNAAEVV NDQAWIEDEF IPTVAKRGAA IIDARGASSA ASAASATIDA
260 270 280 290 300
ARDWLLGTPA DDWVSMAVVS DGSYGVPEGL ISSFPVTTKG GNWTIVSGLE
310 320
IDEFSRGRID KSTAELADER SAVTELGLI
Length:329
Mass (Da):34,322
Last modified:April 16, 2014 - v1
Checksum:i0A512659617F9C37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43996.1.
PIRiG70952.
RefSeqiNP_215756.1. NC_000962.3.
WP_003406301.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP43996; CCP43996; Rv1240.
GeneIDi887119.
KEGGimtu:Rv1240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43996.1.
PIRiG70952.
RefSeqiNP_215756.1. NC_000962.3.
WP_003406301.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4TVOX-ray1.50A/B2-329[»]
ProteinModelPortaliP9WK13.
SMRiP9WK13. Positions 3-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1240.

Proteomic databases

PaxDbiP9WK13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP43996; CCP43996; Rv1240.
GeneIDi887119.
KEGGimtu:Rv1240.

Organism-specific databases

TubercuListiRv1240.

Phylogenomic databases

eggNOGiENOG4105D9Z. Bacteria.
COG0039. LUCA.
KOiK00024.
OMAiAFSQECI.
PhylomeDBiP9WK13.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_MYCTU
AccessioniPrimary (citable) accession number: P9WK13
Secondary accession number(s): L0T626, O54592, P0A5J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: April 13, 2016
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.