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P9WK13

- MDH_MYCTU

UniProt

P9WK13 - MDH_MYCTU

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Protein

Malate dehydrogenase

Gene

mdh

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstrateBy similarity
Binding sitei99 – 991SubstrateBy similarity
Binding sitei106 – 1061NADBy similarity
Binding sitei113 – 1131NADBy similarity
Binding sitei132 – 1321SubstrateBy similarity
Binding sitei163 – 1631SubstrateBy similarity
Active sitei188 – 1881Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NADBy similarity
Nucleotide bindingi130 – 1323NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: MTBBASE

GO - Biological processi

  1. carbohydrate metabolic process Source: MTBBASE
  2. cellular carbohydrate metabolic process Source: InterPro
  3. malate metabolic process Source: InterPro
  4. tricarboxylic acid cycle Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:Rv1240
ORF Names:MTV006.12
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv1240.

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: MTBBASE
  2. cytosol Source: MTBBASE
  3. plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Malate dehydrogenasePRO_0000113380Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP9WK13.
SMRiP9WK13. Positions 4-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

KOiK00024.
PhylomeDBiP9WK13.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WK13 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSASPLKVAV TGAAGQIGYS LLFRLASGSL LGPDRPIELR LLEIEPALQA
60 70 80 90 100
LEGVVMELDD CAFPLLSGVE IGSDPQKIFD GVSLALLVGA RPRGAGMERS
110 120 130 140 150
DLLEANGAIF TAQGKALNAV AADDVRVGVT GNPANTNALI AMTNAPDIPR
160 170 180 190 200
ERFSALTRLD HNRAISQLAA KTGAAVTDIK KMTIWGNHSA TQYPDLFHAE
210 220 230 240 250
VAGKNAAEVV NDQAWIEDEF IPTVAKRGAA IIDARGASSA ASAASATIDA
260 270 280 290 300
ARDWLLGTPA DDWVSMAVVS DGSYGVPEGL ISSFPVTTKG GNWTIVSGLE
310 320
IDEFSRGRID KSTAELADER SAVTELGLI
Length:329
Mass (Da):34,322
Last modified:April 16, 2014 - v1
Checksum:i0A512659617F9C37
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP43996.1.
PIRiG70952.
RefSeqiNP_215756.1. NC_000962.3.
YP_006514615.1. NC_018143.2.

Genome annotation databases

GeneIDi13319819.
887119.
KEGGimtu:Rv1240.
mtv:RVBD_1240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP43996.1 .
PIRi G70952.
RefSeqi NP_215756.1. NC_000962.3.
YP_006514615.1. NC_018143.2.

3D structure databases

ProteinModelPortali P9WK13.
SMRi P9WK13. Positions 4-329.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13319819.
887119.
KEGGi mtu:Rv1240.
mtv:RVBD_1240.

Organism-specific databases

TubercuListi Rv1240.

Phylogenomic databases

KOi K00024.
PhylomeDBi P9WK13.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiMDH_MYCTU
AccessioniPrimary (citable) accession number: P9WK13
Secondary accession number(s): L0T626, O54592, P0A5J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 29, 2014
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3