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Protein

Probable methylmalonyl-CoA mutase large subunit

Gene

mutB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates.By similarity

Catalytic activityi

(R)-methylmalonyl-CoA = succinyl-CoA.

Cofactori

Pathwayi: propanoyl-CoA degradation

This protein is involved in step 3 of the subpathway that synthesizes succinyl-CoA from propanoyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable propionyl-CoA carboxylase beta chain 5 (accD5), Probable propionyl-CoA carboxylase beta chain 6 (accD6)
  2. no protein annotated in this organism
  3. Probable methylmalonyl-CoA mutase small subunit (mutA), Probable methylmalonyl-CoA mutase large subunit (mutB)
This subpathway is part of the pathway propanoyl-CoA degradation, which is itself part of Metabolic intermediate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinyl-CoA from propanoyl-CoA, the pathway propanoyl-CoA degradation and in Metabolic intermediate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi629 – 6291Cobalt (cobalamin axial ligand)

GO - Molecular functioni

GO - Biological processi

  • propionate metabolic process, methylmalonyl pathway Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Cobalamin, Cobalt, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00945; UER00910.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methylmalonyl-CoA mutase large subunit (EC:5.4.99.2)
Short name:
MCM
Gene namesi
Name:mutB
Ordered Locus Names:Rv1493
ORF Names:MTCY277.15
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1493.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Probable methylmalonyl-CoA mutase large subunitPRO_0000194270Add
BLAST

Proteomic databases

PaxDbiP9WJK5.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.By similarity

Protein-protein interaction databases

STRINGi83332.Rv1493.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SE5model-A1-750[»]
ProteinModelPortaliP9WJK5.
SMRiP9WJK5. Positions 22-745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini616 – 748133B12-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni587 – 62640Methylmalonyl-CoA-binding siteBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the methylmalonyl-CoA mutase family.Curated
Contains 1 B12-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105D5P. Bacteria.
COG1884. LUCA.
COG2185. LUCA.
KOiK01847.
OMAiGVNKYRP.
PhylomeDBiP9WJK5.

Family and domain databases

Gene3Di3.20.20.240. 1 hit.
3.40.50.280. 1 hit.
InterProiIPR006159. Acid_CoA_mut_C.
IPR016176. Cbl-dep_enz_cat.
IPR014348. Cbl-dep_enz_cat-sub.
IPR006158. Cobalamin-bd.
IPR006099. MeMalonylCoA_mutase_a/b_cat.
IPR006098. MMCoA_mutase_a_cat.
[Graphical view]
PfamiPF02310. B12-binding. 1 hit.
PF01642. MM_CoA_mutase. 1 hit.
[Graphical view]
SUPFAMiSSF51703. SSF51703. 1 hit.
SSF52242. SSF52242. 1 hit.
TIGRFAMsiTIGR00640. acid_CoA_mut_C. 1 hit.
TIGR00641. acid_CoA_mut_N. 1 hit.
PROSITEiPS51332. B12_BINDING. 1 hit.
PS00544. METMALONYL_COA_MUTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WJK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKTPVIGS FAGVPLHSER AAQSPTEAAV HTHVAAAAAA HGYTPEQLVW
60 70 80 90 100
HTPEGIDVTP VYIAADRAAA EAEGYPLHSF PGEPPFVRGP YPTMYVNQPW
110 120 130 140 150
TIRQYAGFST AADSNAFYRR NLAAGQKGLS VAFDLATHRG YDSDHPRVQG
160 170 180 190 200
DVGMAGVAID SILDMRQLFD GIDLSTVSVS MTMNGAVLPI LALYVVAAEE
210 220 230 240 250
QGVAPEQLAG TIQNDILKEF MVRNTYIYPP KPSMRIISDI FAYTSAKMPK
260 270 280 290 300
FNSISISGYH IQEAGATADL ELAYTLADGV DYIRAGLNAG LDIDSFAPRL
310 320 330 340 350
SFFWGIGMNF FMEVAKLRAG RLLWSELVAQ FAPKSAKSLS LRTHSQTSGW
360 370 380 390 400
SLTAQDVFNN VARTCIEAMA ATQGHTQSLH TNALDEALAL PTDFSARIAR
410 420 430 440 450
NTQLVLQQES GTTRPIDPWG GSYYVEWLTH RLARRARAHI AEVAEHGGMA
460 470 480 490 500
QAISDGIPKL RIEEAAARTQ ARIDSGQQPV VGVNKYQVPE DHEIEVLKVE
510 520 530 540 550
NSRVRAEQLA KLQRLRAGRD EPAVRAALAE LTRAAAEQGR AGADGLGNNL
560 570 580 590 600
LALAIDAARA QATVGEISEA LEKVYGRHRA EIRTISGVYR DEVGKAPNIA
610 620 630 640 650
AATELVEKFA EADGRRPRIL IAKMGQDGHD RGQKVIATAF ADIGFDVDVG
660 670 680 690 700
SLFSTPEEVA RQAADNDVHV IGVSSLAAGH LTLVPALRDA LAQVGRPDIM
710 720 730 740 750
IVVGGVIPPG DFDELYAAGA TAIFPPGTVI ADAAIDLLHR LAERLGYTLD
Length:750
Mass (Da):80,604
Last modified:April 16, 2014 - v1
Checksum:i9E6B866CE507D022
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44254.1.
PIRiH70711.
RefSeqiNP_216009.1. NC_000962.3.
WP_003407587.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44254; CCP44254; Rv1493.
GeneIDi886509.
KEGGimtu:Rv1493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44254.1.
PIRiH70711.
RefSeqiNP_216009.1. NC_000962.3.
WP_003407587.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SE5model-A1-750[»]
ProteinModelPortaliP9WJK5.
SMRiP9WJK5. Positions 22-745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1493.

Proteomic databases

PaxDbiP9WJK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP44254; CCP44254; Rv1493.
GeneIDi886509.
KEGGimtu:Rv1493.

Organism-specific databases

TubercuListiRv1493.

Phylogenomic databases

eggNOGiENOG4105D5P. Bacteria.
COG1884. LUCA.
COG2185. LUCA.
KOiK01847.
OMAiGVNKYRP.
PhylomeDBiP9WJK5.

Enzyme and pathway databases

UniPathwayiUPA00945; UER00910.

Family and domain databases

Gene3Di3.20.20.240. 1 hit.
3.40.50.280. 1 hit.
InterProiIPR006159. Acid_CoA_mut_C.
IPR016176. Cbl-dep_enz_cat.
IPR014348. Cbl-dep_enz_cat-sub.
IPR006158. Cobalamin-bd.
IPR006099. MeMalonylCoA_mutase_a/b_cat.
IPR006098. MMCoA_mutase_a_cat.
[Graphical view]
PfamiPF02310. B12-binding. 1 hit.
PF01642. MM_CoA_mutase. 1 hit.
[Graphical view]
SUPFAMiSSF51703. SSF51703. 1 hit.
SSF52242. SSF52242. 1 hit.
TIGRFAMsiTIGR00640. acid_CoA_mut_C. 1 hit.
TIGR00641. acid_CoA_mut_N. 1 hit.
PROSITEiPS51332. B12_BINDING. 1 hit.
PS00544. METMALONYL_COA_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiMUTB_MYCTU
AccessioniPrimary (citable) accession number: P9WJK5
Secondary accession number(s): L0T9K4, P65487, P71774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 17, 2016
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.