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Protein

Nicotinate-nucleotide pyrophosphorylase [carboxylating]

Gene

nadC

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of quinolinic acid (QA).By similarity

Catalytic activityi

Beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate.
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from quinolinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105Substrate1 Publication1
Binding sitei162Substrate1 Publication1
Binding sitei172Substrate1 Publication1
Binding sitei201Substrate1 Publication1
Binding sitei222Substrate1 Publication1

GO - Molecular functioni

  • nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: MTBBASE

GO - Biological processi

  • NAD biosynthetic process Source: MTBBASE
  • quinolinate catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-5784-MONOMER.
UniPathwayiUPA00253; UER00331.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate-nucleotide pyrophosphorylase [carboxylating] (EC:2.4.2.19)
Alternative name(s):
Quinolinate phosphoribosyltransferase [decarboxylating]
Short name:
QAPRTase
Gene namesi
Name:nadC
Ordered Locus Names:Rv1596
ORF Names:MTCY336.08c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1596.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytoplasm Source: GO_Central
  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001559461 – 285Nicotinate-nucleotide pyrophosphorylase [carboxylating]Add BLAST285

Proteomic databases

PaxDbiP9WJJ7.

Interactioni

Subunit structurei

Homodimer. Hexamer formed by 3 homodimers.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv1596.

Structurei

Secondary structure

1285
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 23Combined sources19
Helixi29 – 34Combined sources6
Beta strandi40 – 49Combined sources10
Helixi56 – 67Combined sources12
Beta strandi71 – 77Combined sources7
Beta strandi89 – 96Combined sources8
Helixi97 – 127Combined sources31
Turni128 – 130Combined sources3
Beta strandi134 – 136Combined sources3
Turni143 – 145Combined sources3
Helixi146 – 155Combined sources10
Beta strandi164 – 171Combined sources8
Helixi173 – 179Combined sources7
Helixi182 – 192Combined sources11
Beta strandi198 – 204Combined sources7
Helixi205 – 211Combined sources7
Helixi212 – 214Combined sources3
Beta strandi217 – 223Combined sources7
Helixi226 – 239Combined sources14
Beta strandi244 – 250Combined sources7
Turni253 – 255Combined sources3
Helixi256 – 261Combined sources6
Beta strandi265 – 268Combined sources4
Helixi270 – 272Combined sources3
Beta strandi274 – 276Combined sources3
Beta strandi281 – 284Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QPNX-ray2.60A/B/C/D/E/F2-285[»]
1QPOX-ray2.40A/B/C/D/E/F2-285[»]
1QPQX-ray2.45A/B/C/D/E/F2-285[»]
1QPRX-ray2.45A/B/C/D/E/F2-285[»]
ProteinModelPortaliP9WJJ7.
SMRiP9WJJ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni138 – 140Substrate binding3
Regioni248 – 250Substrate binding3
Regioni269 – 271Substrate binding3

Sequence similaritiesi

Belongs to the NadC/ModD family.Curated

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
KOiK00767.
OMAiDMIMLKD.
PhylomeDBiP9WJJ7.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WJJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSDWELAA ARAAIARGLD EDLRYGPDVT TLATVPASAT TTASLVTREA
60 70 80 90 100
GVVAGLDVAL LTLNEVLGTN GYRVLDRVED GARVPPGEAL MTLEAQTRGL
110 120 130 140 150
LTAERTMLNL VGHLSGIATA TAAWVDAVRG TKAKIRDTRK TLPGLRALQK
160 170 180 190 200
YAVRTGGGVN HRLGLGDAAL IKDNHVAAAG SVVDALRAVR NAAPDLPCEV
210 220 230 240 250
EVDSLEQLDA VLPEKPELIL LDNFAVWQTQ TAVQRRDSRA PTVMLESSGG
260 270 280
LSLQTAATYA ETGVDYLAVG ALTHSVRVLD IGLDM
Length:285
Mass (Da):29,951
Last modified:April 16, 2014 - v1
Checksum:i45DE3335EC1C522F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44360.1.
PIRiF70543.
RefSeqiNP_216112.1. NC_000962.3.
WP_003898939.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44360; CCP44360; Rv1596.
GeneIDi886281.
KEGGimtu:Rv1596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44360.1.
PIRiF70543.
RefSeqiNP_216112.1. NC_000962.3.
WP_003898939.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QPNX-ray2.60A/B/C/D/E/F2-285[»]
1QPOX-ray2.40A/B/C/D/E/F2-285[»]
1QPQX-ray2.45A/B/C/D/E/F2-285[»]
1QPRX-ray2.45A/B/C/D/E/F2-285[»]
ProteinModelPortaliP9WJJ7.
SMRiP9WJJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1596.

Proteomic databases

PaxDbiP9WJJ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP44360; CCP44360; Rv1596.
GeneIDi886281.
KEGGimtu:Rv1596.

Organism-specific databases

TubercuListiRv1596.

Phylogenomic databases

eggNOGiENOG4105D18. Bacteria.
COG0157. LUCA.
KOiK00767.
OMAiDMIMLKD.
PhylomeDBiP9WJJ7.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00331.
BioCyciMTBH37RV:G185E-5784-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.90.1170.20. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004393. NadC.
IPR027277. NadC/ModD.
IPR002638. Quinolinate_PRibosylTrfase_C.
IPR022412. Quinolinate_PRibosylTrfase_N.
[Graphical view]
PfamiPF01729. QRPTase_C. 1 hit.
PF02749. QRPTase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006250. NadC_ModD. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR00078. nadC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADC_MYCTU
AccessioniPrimary (citable) accession number: P9WJJ7
Secondary accession number(s): L0T8R5, O06594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.