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Protein

Aspartate carbamoyltransferase

Gene

pyrB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Was identified as a high-confidence drug target.

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA)
  2. Aspartate carbamoyltransferase (pyrB), Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC), Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPyrimidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:pyrB
Ordered Locus Names:Rv1380
ORF Names:MTCY02B12.14
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1380

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • plasma membrane Source: MTBBASE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001131631 – 319Aspartate carbamoyltransferaseAdd BLAST319

Proteomic databases

PaxDbiP9WIT7
PRIDEiP9WIT7

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv1380

Structurei

3D structure databases

ProteinModelPortaliP9WIT7
SMRiP9WIT7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105CXT Bacteria
COG0540 LUCA
KOiK00609
OMAiWCDVANM
PhylomeDBiP9WIT7

Family and domain databases

Gene3Di3.40.50.1370, 3 hits
HAMAPiMF_00001 Asp_carb_tr, 1 hit
InterProiView protein in InterPro
IPR006132 Asp/Orn_carbamoyltranf_P-bd
IPR006130 Asp/Orn_carbamoylTrfase
IPR036901 Asp/Orn_carbamoylTrfase_sf
IPR002082 Asp_carbamoyltransf
IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
PANTHERiPTHR11405:SF16 PTHR11405:SF16, 1 hit
PfamiView protein in Pfam
PF00185 OTCace, 1 hit
PF02729 OTCace_N, 1 hit
PRINTSiPR00100 AOTCASE
PR00101 ATCASE
SUPFAMiSSF53671 SSF53671, 1 hit
TIGRFAMsiTIGR00670 asp_carb_tr, 1 hit
PROSITEiView protein in PROSITE
PS00097 CARBAMOYLTRANSFERASE, 1 hit

Sequencei

Sequence statusi: Complete.

P9WIT7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPRHLLTAA DLSRDDATAI LDDADRFAQA LVGRDIKKLP TLRGRTVVTM
60 70 80 90 100
FYENSTRTRV SFEVAGKWMS ADVINVSAAG SSVGKGESLR DTALTLRAAG
110 120 130 140 150
ADALIIRHPA SGAAHLLAQW TGAHNDGPAV INAGDGTHEH PTQALLDALT
160 170 180 190 200
IRQRLGGIEG RRIVIVGDIL HSRVARSNVM LLDTLGAEVV LVAPPTLLPV
210 220 230 240 250
GVTGWPATVS HDFDAELPAA DAVLMLRVQA ERMNGGFFPS VREYSVRYGL
260 270 280 290 300
TERRQAMLPG HAVVLHPGPM VRGMEITSSV ADSSQSAVLQ QVSNGVQVRM
310
AVLFHVLVGA QDAGKEGAA
Length:319
Mass (Da):33,819
Last modified:April 16, 2014 - v1
Checksum:i3D1F8C52A48F95AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP44139.1
PIRiA70959
RefSeqiNP_215896.1, NC_000962.3
WP_003407200.1, NZ_KK339370.1

Genome annotation databases

EnsemblBacteriaiCCP44139; CCP44139; Rv1380
GeneIDi886771
KEGGimtu:Rv1380

Similar proteinsi

Entry informationi

Entry nameiPYRB_MYCTU
AccessioniPrimary (citable) accession number: P9WIT7
Secondary accession number(s): L0T981, P65613, P71808
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: May 23, 2018
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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