Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P9WI57

- PNP_MYCTU

UniProt

P9WI57 - PNP_MYCTU

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 6 (01 Oct 2014)
      Sequence version 1 (16 Apr 2014)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi529 – 5291MagnesiumUniRule annotation
    Metal bindingi535 – 5351MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:Rv2783c
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001584: Chromosome

    Organism-specific databases

    TubercuListiRv2783c.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cell wall Source: MTBBASE
    2. cytosol Source: MTBBASE
    3. plasma membrane Source: MTBBASE

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 752752Polyribonucleotide nucleotidyltransferasePRO_0000329724Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP9WI57.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini595 – 65460KHUniRule annotationAdd
    BLAST
    Domaini666 – 73570S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    KOiK00962.
    PhylomeDBiP9WI57.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P9WI57-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAAEIDEGV FETTATIDNG SFGTRTIRFE TGRLALQAAG AVVAYLDDDN    50
    MLLSATTASK NPKEHFDFFP LTVDVEERMY AAGRIPGSFF RREGRPSTDA 100
    ILTCRLIDRP LRPSFVDGLR NEIQIVVTIL SLDPGDLYDV LAINAASAST 150
    QLGGLPFSGP IGGVRVALID GTWVGFPTVD QIERAVFDMV VAGRIVEGDV 200
    AIMMVEAEAT ENVVELVEGG AQAPTESVVA AGLEAAKPFI AALCTAQQEL 250
    ADAAGKSGKP TVDFPVFPDY GEDVYYSVSS VATDELAAAL TIGGKAERDQ 300
    RIDEIKTQVV QRLADTYEGR EKEVGAALRA LTKKLVRQRI LTDHFRIDGR 350
    GITDIRALSA EVAVVPRAHG SALFERGETQ ILGVTTLDMI KMAQQIDSLG 400
    PETSKRYMHH YNFPPFSTGE TGRVGSPKRR EIGHGALAER ALVPVLPSVE 450
    EFPYAIRQVS EALGSNGSTS MGSVCASTLA LLNAGVPLKA PVAGIAMGLV 500
    SDDIQVEGAV DGVVERRFVT LTDILGAEDA FGDMDFKVAG TKDFVTALQL 550
    DTKLDGIPSQ VLAGALEQAK DARLTILEVM AEAIDRPDEM SPYAPRVTTI 600
    KVPVDKIGEV IGPKGKVINA ITEETGAQIS IEDDGTVFVG ATDGPSAQAA 650
    IDKINAIANP QLPTVGERFL GTVVKTTDFG AFVSLLPGRD GLVHISKLGK 700
    GKRIAKVEDV VNVGDKLRVE IADIDKRGKI SLILVADEDS TAAATDAATV 750
    TS 752
    Length:752
    Mass (Da):79,735
    Last modified:April 16, 2014 - v1
    Checksum:iFA3C5A2D0855A44F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL123456 Genomic DNA. Translation: CCP45582.1.
    PIRiF70883.
    RefSeqiNP_217299.1. NC_000962.3.
    YP_006516229.1. NC_018143.2.

    Genome annotation databases

    GeneIDi13317568.
    888467.
    KEGGimtu:Rv2783c.
    mtv:RVBD_2783c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL123456 Genomic DNA. Translation: CCP45582.1 .
    PIRi F70883.
    RefSeqi NP_217299.1. NC_000962.3.
    YP_006516229.1. NC_018143.2.

    3D structure databases

    ProteinModelPortali P9WI57.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13317568.
    888467.
    KEGGi mtu:Rv2783c.
    mtv:RVBD_2783c.

    Organism-specific databases

    TubercuListi Rv2783c.

    Phylogenomic databases

    KOi K00962.
    PhylomeDBi P9WI57.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR014069. pppGpp_PNP.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    TIGR02696. pppGpp_PNP. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25618 / H37Rv.
    2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 25618 / H37Rv.

    Entry informationi

    Entry nameiPNP_MYCTU
    AccessioniPrimary (citable) accession number: P9WI57
    Secondary accession number(s): L0TDJ4, O33325, Q7D6L0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 1, 2014
    This is version 6 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3