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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi529MagnesiumUniRule annotation1
Metal bindingi535MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciMTBCDC1551:GT3Z-7322-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:MT2853
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001020 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004280651 – 752Polyribonucleotide nucleotidyltransferaseAdd BLAST752

Structurei

3D structure databases

ProteinModelPortaliP9WI56.
SMRiP9WI56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini595 – 654KHUniRule annotationAdd BLAST60
Domaini666 – 735S1 motifUniRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

KOiK00962.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WI56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAEIDEGV FETTATIDNG SFGTRTIRFE TGRLALQAAG AVVAYLDDDN
60 70 80 90 100
MLLSATTASK NPKEHFDFFP LTVDVEERMY AAGRIPGSFF RREGRPSTDA
110 120 130 140 150
ILTCRLIDRP LRPSFVDGLR NEIQIVVTIL SLDPGDLYDV LAINAASAST
160 170 180 190 200
QLGGLPFSGP IGGVRVALID GTWVGFPTVD QIERAVFDMV VAGRIVEGDV
210 220 230 240 250
AIMMVEAEAT ENVVELVEGG AQAPTESVVA AGLEAAKPFI AALCTAQQEL
260 270 280 290 300
ADAAGKSGKP TVDFPVFPDY GEDVYYSVSS VATDELAAAL TIGGKAERDQ
310 320 330 340 350
RIDEIKTQVV QRLADTYEGR EKEVGAALRA LTKKLVRQRI LTDHFRIDGR
360 370 380 390 400
GITDIRALSA EVAVVPRAHG SALFERGETQ ILGVTTLDMI KMAQQIDSLG
410 420 430 440 450
PETSKRYMHH YNFPPFSTGE TGRVGSPKRR EIGHGALAER ALVPVLPSVE
460 470 480 490 500
EFPYAIRQVS EALGSNGSTS MGSVCASTLA LLNAGVPLKA PVAGIAMGLV
510 520 530 540 550
SDDIQVEGAV DGVVERRFVT LTDILGAEDA FGDMDFKVAG TKDFVTALQL
560 570 580 590 600
DTKLDGIPSQ VLAGALEQAK DARLTILEVM AEAIDRPDEM SPYAPRVTTI
610 620 630 640 650
KVPVDKIGEV IGPKGKVINA ITEETGAQIS IEDDGTVFVG ATDGPSAQAA
660 670 680 690 700
IDKINAIANP QLPTVGERFL GTVVKTTDFG AFVSLLPGRD GLVHISKLGK
710 720 730 740 750
GKRIAKVEDV VNVGDKLRVE IADIDKRGKI SLILVADEDS TAAATDAATV

TS
Length:752
Mass (Da):79,735
Last modified:April 16, 2014 - v1
Checksum:iFA3C5A2D0855A44F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK47172.1.
PIRiF70883.
RefSeqiWP_003414124.1. NZ_KK341227.1.

Genome annotation databases

EnsemblBacteriaiAAK47172; AAK47172; MT2853.
KEGGimtc:MT2853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK47172.1.
PIRiF70883.
RefSeqiWP_003414124.1. NZ_KK341227.1.

3D structure databases

ProteinModelPortaliP9WI56.
SMRiP9WI56.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK47172; AAK47172; MT2853.
KEGGimtc:MT2853.

Phylogenomic databases

KOiK00962.
OrthoDBiPOG091H00M0.

Enzyme and pathway databases

BioCyciMTBCDC1551:GT3Z-7322-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_MYCTO
AccessioniPrimary (citable) accession number: P9WI56
Secondary accession number(s): L0TDJ4, O33325, Q7D6L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.