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Protein

Proteasome subunit alpha

Gene

prcA

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Enzyme regulationi

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.

Pathwayi: proteasomal Pup-dependent pathway

This protein is involved in the pathway proteasomal Pup-dependent pathway, which is part of Protein degradation.
View all proteins of this organism that are known to be involved in the pathway proteasomal Pup-dependent pathway and in Protein degradation.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease
Biological processVirulence

Enzyme and pathway databases

UniPathwayiUPA00997.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit
Proteasome core protein PrcA
Gene namesi
Name:prcA
Ordered Locus Names:MT2169
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001020 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004281232 – 248Proteasome subunit alphaAdd BLAST247

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine; partialBy similarity1

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.

Structurei

3D structure databases

ProteinModelPortaliP9WHU0.
SMRiP9WHU0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.

Phylogenomic databases

KOiK03432.
OrthoDBiPOG091H08XR.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_B. Proteasome_A_B. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR023332. Proteasome_alpha-type.
IPR022296. Proteasome_asu_bac.
IPR001353. Proteasome_sua/b.
PANTHERiPTHR11599:SF69. PTHR11599:SF69. 1 hit.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03691. 20S_bact_alpha. 1 hit.
PROSITEiView protein in PROSITE
PS51475. PROTEASOME_ALPHA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WHU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFPYFISPE QAMRERSELA RKGIARAKSV VALAYAGGVL FVAENPSRSL
60 70 80 90 100
QKISELYDRV GFAAAGKFNE FDNLRRGGIQ FADTRGYAYD RRDVTGRQLA
110 120 130 140 150
NVYAQTLGTI FTEQAKPYEV ELCVAEVAHY GETKPPELYR ITYDGSIADE
160 170 180 190 200
PHFVVMGGTT EPIANALKES YAENASLTDA LRIAVAALRA GSADTSGGDQ
210 220 230 240
PTLGVASLEV AVLDANRPRR AFRRITGSAL QALLVDQESP QSDGESSG
Length:248
Mass (Da):26,822
Last modified:April 16, 2014 - v1
Checksum:i235770EC25DF42E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK46452.1.
PIRiH70511.
RefSeqiWP_003899174.1. NZ_KK341227.1.

Genome annotation databases

EnsemblBacteriaiAAK46452; AAK46452; MT2169.
KEGGimtc:MT2169.
PATRICifig|83331.31.peg.2339.

Similar proteinsi

Entry informationi

Entry nameiPSA_MYCTO
AccessioniPrimary (citable) accession number: P9WHU0
Secondary accession number(s): L0T8P4, O33244, Q7D7I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 25, 2017
This is version 23 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families