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P9WHQ9 (HPRT_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 4. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hypoxanthine-guanine phosphoribosyltransferase

Short name=HGPRT
Short name=HGPRTase
EC=2.4.2.8
Gene names
Name:hpt
Synonyms:hprT
Ordered Locus Names:Rv3624c
ORF Names:MTCY15C10.28
OrganismMycobacterium tuberculosis (strain ATCC 25618 / H37Rv) [Reference proteome] [HAMAP]
Taxonomic identifier83332 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactor

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Sequence caution

The sequence CCP46447.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 202202Hypoxanthine-guanine phosphoribosyltransferase
PRO_0000139609

Regions

Nucleotide binding122 – 13110IMP By similarity
Nucleotide binding181 – 1822IMP By similarity

Sites

Active site1261Proton acceptor By similarity
Metal binding1821Magnesium By similarity
Binding site1541IMP By similarity
Binding site1761IMP; via carbonyl oxygen By similarity

Experimental info

Sequence conflict173 – 1742ND → KT in AAB48624. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P9WHQ9 [UniParc].

Last modified April 16, 2014. Version 1.
Checksum: 17AD6817A2F8ED90

FASTA20222,251
        10         20         30         40         50         60 
MHVTQSSSAI TPGQTAELYP GDIKSVLLTA EQIQARIAEL GEQIGNDYRE LSATTGQDLL 

        70         80         90        100        110        120 
LITVLKGAVL FVTDLARAIP VPTQFEFMAV SSYGSSTSSS GVVRILKDLD RDIHGRDVLI 

       130        140        150        160        170        180 
VEDVVDSGLT LSWLSRNLTS RNPRSLRVCT LLRKPDAVHA NVEIAYVGFD IPNDFVVGYG 

       190        200 
LDYDERYRDL SYIGTLDPRV YQ 

« Hide

References

« Hide 'large scale' references
[1]Bergeson S.E., Barry R., Allen T., Hwang H., Ullman B.
Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[3]"Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry."
Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K., Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R., Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M., Kumar P. expand/collapse author list , Chaerkady R., Ramachandran S., Dash D., Pandey A.
Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U88876 Genomic DNA. Translation: AAB48624.1.
AL123456 Genomic DNA. Translation: CCP46447.1. Different initiation.
PIRA70561.
RefSeqNP_218141.1. NC_000962.3.
YP_006517113.1. NC_018143.2.

3D structure databases

ProteinModelPortalP9WHQ9.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID885398.

Organism-specific databases

TubercuListRv3624c.

Enzyme and pathway databases

UniPathwayUPA00591; UER00648.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
InterProIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMSSF53271. SSF53271. 1 hit.
TIGRFAMsTIGR01203. HGPRTase. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHPRT_MYCTU
AccessionPrimary (citable) accession number: P9WHQ9
Secondary accession number(s): L0TER1 expand/collapse secondary AC list , O06383, P0A5T0, P96906
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: July 9, 2014
This is version 4 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names