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Protein

tRNA pseudouridine synthase B

Gene

truB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.UniRule annotation

Catalytic activityi

tRNA uridine(55) = tRNA pseudouridine(55).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei42 – 421NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine synthase BUniRule annotation (EC:5.4.99.25UniRule annotation)
Alternative name(s):
MTB-TRUB
tRNA pseudouridine(55) synthaseUniRule annotation
Short name:
Psi55 synthaseUniRule annotation
tRNA pseudouridylate synthaseUniRule annotation
tRNA-uridine isomeraseUniRule annotation
Gene namesi
Name:truBUniRule annotation
Ordered Locus Names:Rv2793c
ORF Names:MTV002.58c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2793c.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298tRNA pseudouridine synthase BPRO_0000121872Add
BLAST

Proteomic databases

PaxDbiP9WHP7.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv2793c.

Structurei

Secondary structure

1
298
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 138Combined sources
Helixi19 – 2911Combined sources
Beta strandi35 – 395Combined sources
Beta strandi46 – 538Combined sources
Helixi54 – 629Combined sources
Turni63 – 653Combined sources
Beta strandi68 – 769Combined sources
Beta strandi78 – 803Combined sources
Beta strandi89 – 924Combined sources
Helixi100 – 10910Combined sources
Beta strandi112 – 1143Combined sources
Beta strandi148 – 16114Combined sources
Beta strandi164 – 17411Combined sources
Helixi179 – 18911Combined sources
Beta strandi194 – 20411Combined sources
Helixi209 – 2113Combined sources
Helixi215 – 2206Combined sources
Beta strandi225 – 2273Combined sources
Helixi228 – 2358Combined sources
Beta strandi236 – 2405Combined sources
Helixi243 – 2508Combined sources
Beta strandi264 – 2674Combined sources
Beta strandi273 – 2808Combined sources
Beta strandi283 – 2908Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGVX-ray1.90A/B1-298[»]
ProteinModelPortaliP9WHP7.
SMRiP9WHP7. Positions 1-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0T. Bacteria.
COG0130. LUCA.
KOiK03177.
OMAiKEYISEF.
PhylomeDBiP9WHP7.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR015947. PUA-like_domain.
IPR015225. tRNA_psdUridine_synth_fam2_C.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09142. TruB_C. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WHP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATGPGIVV IDKPAGMTSH DVVGRCRRIF ATRRVGHAGT LDPMATGVLV
60 70 80 90 100
IGIERATKIL GLLTAAPKSY AATIRLGQTT STEDAEGQVL QSVPAKHLTI
110 120 130 140 150
EAIDAAMERL RGEIRQVPSS VSAIKVGGRR AYRLARQGRS VQLEARPIRI
160 170 180 190 200
DRFELLAARR RDQLIDIDVE IDCSSGTYIR ALARDLGDAL GVGGHVTALR
210 220 230 240 250
RTRVGRFELD QARSLDDLAE RPALSLSLDE ACLLMFARRD LTAAEASAAA
260 270 280 290
NGRSLPAVGI DGVYAACDAD GRVIALLRDE GSRTRSVAVL RPATMHPG
Length:298
Mass (Da):31,820
Last modified:April 16, 2014 - v1
Checksum:i9F77797DC13B34C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45592.1.
PIRiH70884.
RefSeqiNP_217309.1. NC_000962.3.
WP_003414147.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP45592; CCP45592; Rv2793c.
GeneIDi888587.
KEGGimtu:Rv2793c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45592.1.
PIRiH70884.
RefSeqiNP_217309.1. NC_000962.3.
WP_003414147.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SGVX-ray1.90A/B1-298[»]
ProteinModelPortaliP9WHP7.
SMRiP9WHP7. Positions 1-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2793c.

Proteomic databases

PaxDbiP9WHP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45592; CCP45592; Rv2793c.
GeneIDi888587.
KEGGimtu:Rv2793c.

Organism-specific databases

TubercuListiRv2793c.

Phylogenomic databases

eggNOGiENOG4105D0T. Bacteria.
COG0130. LUCA.
KOiK03177.
OMAiKEYISEF.
PhylomeDBiP9WHP7.

Family and domain databases

HAMAPiMF_01080. TruB_bact.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR015947. PUA-like_domain.
IPR015225. tRNA_psdUridine_synth_fam2_C.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR13767. PTHR13767. 1 hit.
PfamiPF09142. TruB_C. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00431. TruB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  3. "Crystal structure of the apo forms of psi 55 tRNA pseudouridine synthase from Mycobacterium tuberculosis: a hinge at the base of the catalytic cleft."
    Chaudhuri B.N., Chan S., Perry L.J., Yeates T.O.
    J. Biol. Chem. 279:24585-24591(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiTRUB_MYCTU
AccessioniPrimary (citable) accession number: P9WHP7
Secondary accession number(s): L0TDK3, O33335, P62190
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 17, 2016
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.