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Protein

Prokaryotic ubiquitin-like protein Pup

Gene

pup

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation. Among the identified substrates are the FabD, PanB and Mpa proteins (By similarity).By similarity

Pathwayi

GO - Molecular functioni

  1. protein tag Source: UniProtKB-HAMAP

GO - Biological processi

  1. modification-dependent protein catabolic process Source: UniProtKB-HAMAP
  2. proteasomal protein catabolic process Source: UniProtKB-HAMAP
  3. protein pupylation Source: UniProtKB-HAMAP
Complete GO annotation...

Enzyme and pathway databases

UniPathwayiUPA00997.

Names & Taxonomyi

Protein namesi
Recommended name:
Prokaryotic ubiquitin-like protein Pup
Alternative name(s):
Bacterial ubiquitin-like modifier
Gene namesi
Name:pup
Ordered Locus Names:MT2171
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001020 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6464Prokaryotic ubiquitin-like protein PupPRO_0000428154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641Deamidated glutamineBy similarity

Post-translational modificationi

Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process.By similarity

Interactioni

Subunit structurei

Strongly interacts with the proteasome-associated ATPase ARC (Mpa) through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half.By similarity

Structurei

3D structure databases

ProteinModelPortaliP9WHN4.
SMRiP9WHN4. Positions 39-64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 5838Mpa/ARC ATPase bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili23 – 5230Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

KOiK13570.

Family and domain databases

HAMAPiMF_02106. Pup.
InterProiIPR008515. Ubiquitin-like_Pup.
[Graphical view]
PfamiPF05639. Pup. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03687. pupylate_cterm. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WHN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQEQTKRGG GGGDDDDIAG STAAGQERRE KLTEETDDLL DEIDDVLEEN
60
AEDFVRAYVQ KGGQ
Length:64
Mass (Da):6,944
Last modified:April 16, 2014 - v1
Checksum:i7D958B770D8766E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK46454.1.
PIRiB70512.
RefSeqiNP_336640.1. NC_002755.2.

Genome annotation databases

EnsemblBacteriaiAAK46454; AAK46454; MT2171.
KEGGimtc:MT2171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK46454.1.
PIRiB70512.
RefSeqiNP_336640.1. NC_002755.2.

3D structure databases

ProteinModelPortaliP9WHN4.
SMRiP9WHN4. Positions 39-64.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK46454; AAK46454; MT2171.
KEGGimtc:MT2171.

Phylogenomic databases

KOiK13570.

Enzyme and pathway databases

UniPathwayiUPA00997.

Family and domain databases

HAMAPiMF_02106. Pup.
InterProiIPR008515. Ubiquitin-like_Pup.
[Graphical view]
PfamiPF05639. Pup. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03687. pupylate_cterm. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CDC 1551 / Oshkosh.

Entry informationi

Entry nameiPUP_MYCTO
AccessioniPrimary (citable) accession number: P9WHN4
Secondary accession number(s): B6DAC1
, L0TBK1, O33246, Q7D7I1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: April 1, 2015
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.