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P9WHN4 (PUP_MYCTO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 4. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prokaryotic ubiquitin-like protein Pup
Alternative name(s):
Bacterial ubiquitin-like modifier
Gene names
Name:pup
Ordered Locus Names:MT2171
OrganismMycobacterium tuberculosis (strain CDC 1551 / Oshkosh) [Complete proteome] [HAMAP]
Taxonomic identifier83331 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length64 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Protein modifier that is covalently attached to lysine residues of substrate proteins, thereby targeting them for proteasomal degradation. The tagging system is termed pupylation. Among the identified substrates are the FabD, PanB and Mpa proteins By similarity. HAMAP-Rule MF_02106

Pathway

Protein degradation; proteasomal Pup-dependent pathway. HAMAP-Rule MF_02106

Subunit structure

Strongly interacts with the proteasome-associated ATPase ARC (Mpa) through a hydrophobic interface; the interacting region of Pup lies in its C-terminal half By similarity.

Post-translational modification

Is modified by deamidation of its C-terminal glutamine to glutamate by the deamidase Dop, a prerequisite to the subsequent pupylation process By similarity. HAMAP-Rule MF_02106

Sequence similarities

Belongs to the prokaryotic ubiquitin-like protein family.

Ontologies

Keywords
   DomainCoiled coil
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmodification-dependent protein catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

proteasomal protein catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

protein pupylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionprotein tag

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6464Prokaryotic ubiquitin-like protein Pup HAMAP-Rule MF_02106
PRO_0000428154

Regions

Region21 – 5838Mpa/ARC ATPase binding By similarity
Coiled coil23 – 5230 Potential

Amino acid modifications

Modified residue641Deamidated glutamine By similarity

Sequences

Sequence LengthMass (Da)Tools
P9WHN4 [UniParc].

Last modified April 16, 2014. Version 1.
Checksum: 7D958B770D8766E3

FASTA646,944
        10         20         30         40         50         60 
MAQEQTKRGG GGGDDDDIAG STAAGQERRE KLTEETDDLL DEIDDVLEEN AEDFVRAYVQ 


KGGQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000516 Genomic DNA. Translation: AAK46454.1.
PIRB70512.
RefSeqNP_336640.1. NC_002755.2.

3D structure databases

ProteinModelPortalP9WHN4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID924534.

Organism-specific databases

CMRSearch...

Enzyme and pathway databases

UniPathwayUPA00997.

Family and domain databases

HAMAPMF_02106. Pup.
InterProIPR008515. Ubiquitin-like_Pup.
[Graphical view]
PfamPF05639. Pup. 1 hit.
[Graphical view]
TIGRFAMsTIGR03687. pupylate_cterm. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUP_MYCTO
AccessionPrimary (citable) accession number: P9WHN4
Secondary accession number(s): B6DAC1 expand/collapse secondary AC list , L0TBK1, O33246, Q7D7I1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: July 9, 2014
This is version 4 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways