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Protein

Protein RecA

Gene

recA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.
PI-MtuI is an endonuclease.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi67 – 74ATPBy similarity8

GO - Molecular functioni

  • ATPase activity Source: MTBBASE
  • ATP binding Source: MTBBASE
  • DNA-dependent ATPase activity Source: MTBBASE
  • double-stranded DNA binding Source: GO_Central
  • endodeoxyribonuclease activity Source: MTBBASE
  • four-way junction DNA binding Source: GO_Central
  • magnesium ion binding Source: MTBBASE
  • manganese ion binding Source: MTBBASE
  • recombinase activity Source: MTBBASE
  • single-stranded DNA binding Source: MTBBASE

GO - Biological processi

  • cellular response to DNA damage stimulus Source: MTBBASE
  • DNA recombinase assembly Source: GO_Central
  • intein-mediated protein splicing Source: InterPro
  • intron homing Source: UniProtKB-KW
  • mitotic recombination Source: GO_Central
  • recombinational repair Source: MTBBASE
  • response to antibiotic Source: MTBBASE
  • response to ionizing radiation Source: GO_Central
  • SOS response Source: UniProtKB-KW
  • strand invasion Source: MTBBASE
  • UV protection Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Intron homing, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-6985-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecA
Alternative name(s):
Recombinase A
Cleaved into the following chain:
Alternative name(s):
Mtu RecA intein
Gene namesi
Name:recA
Ordered Locus Names:Rv2737c
ORF Names:MTV002.02c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2737c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741171.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302691 – 251Protein RecA, 1st partAdd BLAST251
ChainiPRO_0000030270252 – 691Endonuclease PI-MtuIAdd BLAST440
ChainiPRO_0000030271692 – 790Protein RecA, 2nd partAdd BLAST99

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki762Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Isopeptide bond, Protein splicing, Ubl conjugation

Proteomic databases

PaxDbiP9WHJ3.

Interactioni

Subunit structurei

Interacts with UvrD1 and UvrA.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv2737c.

Chemistry databases

BindingDBiP9WHJ3.

Structurei

Secondary structure

1790
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 22Combined sources18
Helixi24 – 26Combined sources3
Helixi46 – 51Combined sources6
Beta strandi53 – 58Combined sources6
Beta strandi61 – 68Combined sources8
Helixi73 – 86Combined sources14
Beta strandi91 – 97Combined sources7
Helixi102 – 108Combined sources7
Helixi112 – 114Combined sources3
Beta strandi116 – 118Combined sources3
Helixi123 – 135Combined sources13
Beta strandi140 – 145Combined sources6
Helixi147 – 149Combined sources3
Helixi153 – 156Combined sources4
Helixi167 – 186Combined sources20
Beta strandi189 – 195Combined sources7
Helixi213 – 219Combined sources7
Beta strandi221 – 234Combined sources14
Beta strandi237 – 251Combined sources15
Beta strandi258 – 260Combined sources3
Turni262 – 264Combined sources3
Beta strandi267 – 269Combined sources3
Helixi270 – 275Combined sources6
Beta strandi281 – 285Combined sources5
Beta strandi287 – 289Combined sources3
Beta strandi291 – 312Combined sources22
Beta strandi317 – 320Combined sources4
Beta strandi325 – 328Combined sources4
Beta strandi331 – 334Combined sources4
Helixi335 – 337Combined sources3
Beta strandi343 – 347Combined sources5
Turni349 – 351Combined sources3
Beta strandi655 – 676Combined sources22
Turni677 – 679Combined sources3
Beta strandi681 – 684Combined sources4
Beta strandi687 – 690Combined sources4
Beta strandi698 – 704Combined sources7
Turni705 – 707Combined sources3
Helixi711 – 721Combined sources11
Beta strandi724 – 728Combined sources5
Beta strandi731 – 734Combined sources4
Beta strandi737 – 742Combined sources6
Helixi743 – 752Combined sources10
Helixi754 – 767Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G18X-ray3.80A1-790[»]
1G19X-ray3.00A1-790[»]
1MO3X-ray3.10A1-790[»]
1MO4X-ray3.20A1-790[»]
1MO5X-ray3.25A1-790[»]
1MO6X-ray3.20A1-790[»]
2IMZX-ray1.70A/B252-691[»]
2IN0X-ray1.60A252-691[»]
2IN8X-ray1.70A252-691[»]
2IN9X-ray1.80A252-691[»]
2L8LNMR-A252-345[»]
A654-691[»]
3IFJX-ray1.90A/B252-691[»]
3IGDX-ray2.40A252-691[»]
4OQFX-ray2.80A1-771[»]
4PO1X-ray3.40A1-790[»]
4PO8X-ray2.70A1-790[»]
4PO9X-ray2.75A1-790[»]
4POAX-ray2.95A1-790[»]
4PPFX-ray2.30A1-790[»]
4PPGX-ray3.00A1-790[»]
4PPNX-ray2.60A1-790[»]
4PPQX-ray2.85A1-790[»]
4PQFX-ray2.80A1-790[»]
4PQRX-ray2.80A1-790[»]
4PQYX-ray2.95A1-790[»]
4PR0X-ray2.60A1-790[»]
4PSAX-ray2.65A1-790[»]
4PSKX-ray2.80A1-790[»]
4PSVX-ray2.60A1-790[»]
4PTLX-ray2.50A1-790[»]
5I0AX-ray1.50A252-696[»]
5K08X-ray1.40A252-694[»]
ProteinModelPortaliP9WHJ3.
SMRiP9WHJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini366 – 508DOD-type homing endonucleaseAdd BLAST143

Sequence similaritiesi

Belongs to the RecA family.Curated

Phylogenomic databases

eggNOGiCOG0468. LUCA.
COG1372. LUCA.
KOiK03553.
OMAiFVDFTPL.
PhylomeDBiP9WHJ3.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 2 hits.
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
PR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WHJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV
60 70 80 90 100
ALGIGGLPRG RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL
110 120 130 140 150
DPDYAKKLGV DTDSLLVSQP DTGEQALEIA DMLIRSGALD IVVIDSVAAL
160 170 180 190 200
VPRAELEGEM GDSHVGLQAR LMSQALRKMT GALNNSGTTA IFINQLRDKI
210 220 230 240 250
GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG NRTRVKVVKN
260 270 280 290 300
KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF
310 320 330 340 350
DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF
360 370 380 390 400
DGFGDSAPIP ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR
410 420 430 440 450
IAATLGCAAH PQGRISLAIA HRPGERNGVA DLCQQAGIYG KLAWEKTIPN
460 470 480 490 500
WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ TGALRVGYTT TSEQLAHQIH
510 520 530 540 550
WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR ISGMDNVTAF
560 570 580 590 600
AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG
610 620 630 640 650
VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM
660 670 680 690 700
LAEELRYSVI REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE
710 720 730 740 750
FDILYGKGIS REGSLIDMGV DQGLIRKSGA WFTYEGEQLG QGKENARNFL
760 770 780 790
VENADVADEI EKKIKEKLGI GAVVTDDPSN DGVLPAPVDF
Length:790
Mass (Da):85,389
Last modified:April 16, 2014 - v1
Checksum:iAD16340D2ED5E572
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti305R → Q in strain: Canetti and SO93. 1
Natural varianti430A → L in strain: Canetti and SO93. 1
Natural varianti434 – 435QQ → RR in strain: Canetti and SO93. 2
Natural varianti438 – 439IY → VH in strain: Canetti and SO93. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58485 Genomic DNA. Translation: CAA41395.1.
AJ000012 Genomic DNA. Translation: CAA03857.1.
AJ000011 Genomic DNA. Translation: CAA03856.1.
AL123456 Genomic DNA. Translation: CCP45535.1.
PIRiS18206.
RefSeqiNP_217253.1. NC_000962.3.
WP_003414006.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP45535; CCP45535; Rv2737c.
GeneIDi888371.
KEGGimtu:Rv2737c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58485 Genomic DNA. Translation: CAA41395.1.
AJ000012 Genomic DNA. Translation: CAA03857.1.
AJ000011 Genomic DNA. Translation: CAA03856.1.
AL123456 Genomic DNA. Translation: CCP45535.1.
PIRiS18206.
RefSeqiNP_217253.1. NC_000962.3.
WP_003414006.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G18X-ray3.80A1-790[»]
1G19X-ray3.00A1-790[»]
1MO3X-ray3.10A1-790[»]
1MO4X-ray3.20A1-790[»]
1MO5X-ray3.25A1-790[»]
1MO6X-ray3.20A1-790[»]
2IMZX-ray1.70A/B252-691[»]
2IN0X-ray1.60A252-691[»]
2IN8X-ray1.70A252-691[»]
2IN9X-ray1.80A252-691[»]
2L8LNMR-A252-345[»]
A654-691[»]
3IFJX-ray1.90A/B252-691[»]
3IGDX-ray2.40A252-691[»]
4OQFX-ray2.80A1-771[»]
4PO1X-ray3.40A1-790[»]
4PO8X-ray2.70A1-790[»]
4PO9X-ray2.75A1-790[»]
4POAX-ray2.95A1-790[»]
4PPFX-ray2.30A1-790[»]
4PPGX-ray3.00A1-790[»]
4PPNX-ray2.60A1-790[»]
4PPQX-ray2.85A1-790[»]
4PQFX-ray2.80A1-790[»]
4PQRX-ray2.80A1-790[»]
4PQYX-ray2.95A1-790[»]
4PR0X-ray2.60A1-790[»]
4PSAX-ray2.65A1-790[»]
4PSKX-ray2.80A1-790[»]
4PSVX-ray2.60A1-790[»]
4PTLX-ray2.50A1-790[»]
5I0AX-ray1.50A252-696[»]
5K08X-ray1.40A252-694[»]
ProteinModelPortaliP9WHJ3.
SMRiP9WHJ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2737c.

Chemistry databases

BindingDBiP9WHJ3.
ChEMBLiCHEMBL1741171.

Proteomic databases

PaxDbiP9WHJ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45535; CCP45535; Rv2737c.
GeneIDi888371.
KEGGimtu:Rv2737c.

Organism-specific databases

TubercuListiRv2737c.

Phylogenomic databases

eggNOGiCOG0468. LUCA.
COG1372. LUCA.
KOiK03553.
OMAiFVDFTPL.
PhylomeDBiP9WHJ3.

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-6985-MONOMER.

Miscellaneous databases

PROiP9WHJ3.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 2 hits.
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
PR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECA_MYCTU
AccessioniPrimary (citable) accession number: P9WHJ3
Secondary accession number(s): L0TAH5
, O34519, P0A5U4, P26345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 30, 2016
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.