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Protein

Thioredoxin reductase

Gene

trxB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

The active site is a redox-active disulfide bond.
Was identified as a high-confidence drug target.

Catalytic activityi

Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 51FADBy similarity8
Nucleotide bindingi288 – 297FADBy similarity10

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: MTBBASE
  • NADPH binding Source: MTBBASE
  • protein disulfide oxidoreductase activity Source: MTBBASE
  • thioredoxin-disulfide reductase activity Source: MTBBASE

GO - Biological processi

  • cell redox homeostasis Source: MTBBASE
  • growth Source: MTBBASE
  • removal of superoxide radicals Source: InterPro
  • response to hypoxia Source: MTBBASE

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

ReactomeiR-HSA-1222541 Cell redox homeostasis

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin reductase (EC:1.8.1.9)
Short name:
TR
Short name:
TRXR
Gene namesi
Name:trxB
Synonyms:trxB2
Ordered Locus Names:Rv3913
ORF Names:MTV028.04
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi

Organism-specific databases

TubercuListiRv3913

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2390811

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001667412 – 335Thioredoxin reductaseAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Disulfide bondi145 ↔ 148Redox-activeBy similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP9WHH1
PRIDEiP9WHH1

PTM databases

iPTMnetiP9WHH1

Expressioni

Inductioni

Expressed following oxidative stress under control of SigH.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi83332.Rv3913

Chemistry databases

BindingDBiP9WHH1

Structurei

Secondary structure

1335
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 20Combined sources6
Helixi23 – 35Combined sources13
Beta strandi41 – 43Combined sources3
Helixi51 – 53Combined sources3
Helixi70 – 83Combined sources14
Beta strandi87 – 89Combined sources3
Beta strandi93 – 97Combined sources5
Beta strandi99 – 107Combined sources9
Beta strandi112 – 120Combined sources9
Beta strandi124 – 126Combined sources3
Helixi133 – 136Combined sources4
Turni139 – 141Combined sources3
Beta strandi142 – 144Combined sources3
Helixi146 – 149Combined sources4
Helixi150 – 153Combined sources4
Beta strandi157 – 161Combined sources5
Helixi165 – 174Combined sources10
Turni175 – 177Combined sources3
Beta strandi179 – 184Combined sources6
Beta strandi186 – 189Combined sources4
Helixi196 – 202Combined sources7
Beta strandi206 – 209Combined sources4
Beta strandi211 – 218Combined sources8
Beta strandi220 – 222Combined sources3
Beta strandi225 – 231Combined sources7
Beta strandi237 – 239Combined sources3
Beta strandi244 – 246Combined sources3
Beta strandi250 – 252Combined sources3
Turni255 – 257Combined sources3
Beta strandi275 – 277Combined sources3
Beta strandi283 – 285Combined sources3
Helixi287 – 289Combined sources3
Helixi297 – 320Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A87X-ray3.00A/B1-335[»]
ProteinModelPortaliP9WHH1
SMRiP9WHH1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiENOG4105C3M Bacteria
COG0492 LUCA
KOiK00384
OMAiDPIQGPW
PhylomeDBiP9WHH1

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR008255 Pyr_nucl-diS_OxRdtase_2_AS
IPR000103 Pyridine_nuc-diS_OxRdtase_2
IPR005982 Thioredox_Rdtase
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PRINTSiPR00469 PNDRDTASEII
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR01292 TRX_reduct, 1 hit
PROSITEiView protein in PROSITE
PS00573 PYRIDINE_REDOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WHH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPPVHDRA HHPVRDVIVI GSGPAGYTAA LYAARAQLAP LVFEGTSFGG
60 70 80 90 100
ALMTTTDVEN YPGFRNGITG PELMDEMREQ ALRFGADLRM EDVESVSLHG
110 120 130 140 150
PLKSVVTADG QTHRARAVIL AMGAAARYLQ VPGEQELLGR GVSSCATCDG
160 170 180 190 200
FFFRDQDIAV IGGGDSAMEE ATFLTRFARS VTLVHRRDEF RASKIMLDRA
210 220 230 240 250
RNNDKIRFLT NHTVVAVDGD TTVTGLRVRD TNTGAETTLP VTGVFVAIGH
260 270 280 290 300
EPRSGLVREA IDVDPDGYVL VQGRTTSTSL PGVFAAGDLV DRTYRQAVTA
310 320 330
AGSGCAAAID AERWLAEHAA TGEADSTDAL IGAQR
Length:335
Mass (Da):35,643
Last modified:April 16, 2014 - v1
Checksum:i3D0DD581E6C187E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125A → R in CAA65070 (Ref. 1) Curated1
Sequence conflicti215V → C in CAA65070 (Ref. 1) Curated1
Sequence conflicti228V → Y in CAA65070 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95798 Genomic DNA Translation: CAA65070.1
AL123456 Genomic DNA Translation: CCP46742.1
PIRiA70851
RefSeqiNP_218430.1, NC_000962.3
WP_003900782.1, NZ_KK339374.1

Genome annotation databases

EnsemblBacteriaiCCP46742; CCP46742; Rv3913
GeneIDi886232
KEGGimtu:Rv3913

Similar proteinsi

Entry informationi

Entry nameiTRXB_MYCTU
AccessioniPrimary (citable) accession number: P9WHH1
Secondary accession number(s): L0TFM3, O53592, P52214
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: May 23, 2018
This is version 29 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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