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Protein

Transcription termination factor Rho

Gene

rho

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template. Shows poor RNA-dependent ATP hydrolysis and inefficient DNA-RNA unwinding activities, but exhibits robust and fast transcription termination, which suggests that the transcription termination function of M.tuberculosis Rho is not correlated with its helicase/translocase activities and that these functions may not be important for its RNA release process.UniRule annotation1 Publication

Miscellaneous

Was identified as a high-confidence drug target.

Enzyme regulationi

The antibiotic bicyclomycin inhibits ATPase activity but does not affect termination function.1 Publication

Kineticsi

  1. KM=70 µM for ATP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei387ATPUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi344 – 349ATPUniRule annotation6
    Nucleotide bindingi356 – 361ATPUniRule annotation6

    GO - Molecular functioni

    GO - Biological processi

    • growth Source: MTBBASE
    • regulation of transcription, DNA-templated Source: UniProtKB-KW
    • termination of RNA polymerase I transcription Source: MTBBASE

    Keywordsi

    Molecular functionHelicase, Hydrolase, RNA-binding
    Biological processTranscription, Transcription regulation, Transcription termination
    LigandATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription termination factor RhoUniRule annotation (EC:3.6.4.-UniRule annotation)
    Alternative name(s):
    ATP-dependent helicase RhoUniRule annotation
    Gene namesi
    Name:rhoUniRule annotation
    Ordered Locus Names:Rv1297
    ORF Names:MTCY373.17
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    Proteomesi
    • UP000001584 Componenti: Chromosome

    Organism-specific databases

    TubercuListiRv1297.

    Subcellular locationi

    GO - Cellular componenti

    • cell wall Source: MTBBASE
    • plasma membrane Source: MTBBASE

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001889701 – 602Transcription termination factor RhoAdd BLAST602

    Proteomic databases

    PaxDbiP9WHF3.

    Interactioni

    Subunit structurei

    Homohexamer. The homohexamer assembles into an open ring structure. Does not bind NusG.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi83332.Rv1297.

    Structurei

    3D structure databases

    ProteinModelPortaliP9WHF3.
    SMRiP9WHF3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini223 – 301Rho RNA-BDPROSITE-ProRule annotationAdd BLAST79

    Domaini

    Contains an extra N-terminal domain, a S1-like RNA-binding domain and a conserved P-loop NTPase domain.1 Publication

    Sequence similaritiesi

    Belongs to the Rho family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105C4P. Bacteria.
    COG1158. LUCA.
    KOiK03628.
    OMAiPADDHTT.
    PhylomeDBiP9WHF3.

    Family and domain databases

    HAMAPiMF_01884. Rho. 1 hit.
    InterProiView protein in InterPro
    IPR003593. AAA+_ATPase.
    IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
    IPR011129. CSD.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR011112. Rho_N.
    IPR036269. Rho_N_sf.
    IPR011113. Rho_RNA-bd.
    IPR004665. Term_rho.
    PANTHERiPTHR15184:SF34. PTHR15184:SF34. 1 hit.
    PfamiView protein in Pfam
    PF00006. ATP-synt_ab. 1 hit.
    PF07498. Rho_N. 1 hit.
    PF07497. Rho_RNA_bind. 1 hit.
    SMARTiView protein in SMART
    SM00382. AAA. 1 hit.
    SM00357. CSP. 1 hit.
    SM00959. Rho_N. 1 hit.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF68912. SSF68912. 1 hit.
    TIGRFAMsiTIGR00767. rho. 1 hit.
    PROSITEiView protein in PROSITE
    PS51856. RHO_RNA_BD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P9WHF3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTDTDLITAG ESTDGKPSDA AATDPPDLNA DEPAGSLATM VLPELRALAN
    60 70 80 90 100
    RAGVKGTSGM RKNELIAAIE EIRRQANGAP AVDRSAQEHD KGDRPPSSEA
    110 120 130 140 150
    PATQGEQTPT EQIDSQSQQV RPERRSATRE AGPSGSGERA GTAADDTDNR
    160 170 180 190 200
    QGGQQDAKTE ERGTDAGGDQ GGDQQASGGQ QARGDEDGEA RQGRRGRRFR
    210 220 230 240 250
    DRRRRGERSG DGAEAELRED DVVQPVAGIL DVLDNYAFVR TSGYLPGPHD
    260 270 280 290 300
    VYVSMNMVRK NGMRRGDAVT GAVRVPKEGE QPNQRQKFNP LVRLDSINGG
    310 320 330 340 350
    SVEDAKKRPE FGKLTPLYPN QRLRLETSTE RLTTRVIDLI MPIGKGQRAL
    360 370 380 390 400
    IVSPPKAGKT TILQDIANAI TRNNPECHLM VVLVDERPEE VTDMQRSVKG
    410 420 430 440 450
    EVIASTFDRP PSDHTSVAEL AIERAKRLVE QGKDVVVLLD SITRLGRAYN
    460 470 480 490 500
    NASPASGRIL SGGVDSTALY PPKRFLGAAR NIEEGGSLTI IATAMVETGS
    510 520 530 540 550
    TGDTVIFEEF KGTGNAELKL DRKIAERRVF PAVDVNPSGT RKDELLLSPD
    560 570 580 590 600
    EFAIVHKLRR VLSGLDSHQA IDLLMSQLRK TKNNYEFLVQ VSKTTPGSMD

    SD
    Length:602
    Mass (Da):65,133
    Last modified:April 16, 2014 - v1
    Checksum:i4E77B200810AE57F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA. Translation: CCP44054.1.
    PIRiE70773.
    RefSeqiNP_215813.1. NC_000962.3.
    WP_003898814.1. NZ_KK339370.1.

    Genome annotation databases

    EnsemblBacteriaiCCP44054; CCP44054; Rv1297.
    GeneIDi886952.
    KEGGimtu:Rv1297.

    Similar proteinsi

    Entry informationi

    Entry nameiRHO_MYCTU
    AccessioniPrimary (citable) accession number: P9WHF3
    Secondary accession number(s): L0T8Z1, P66028, Q10607
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: March 28, 2018
    This is version 22 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome