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Protein

30S ribosomal protein S12

Gene

rpsL

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

With S4 and S5 plays an important role in translational accuracy.By similarity
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • growth Source: MTBBASE
  • response to antibiotic Source: UniProtKB-KW
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

Antibiotic resistance

Keywords - Ligandi

RNA-binding, rRNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S12
Gene namesi
Name:rpsL
Synonyms:rps12
Ordered Locus Names:Rv0682
ORF Names:MTV040.10
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0682.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2363965.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12412430S ribosomal protein S12PRO_0000146272Add
BLAST

Proteomic databases

PaxDbiP9WH63.

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi83332.Rv0682.

Structurei

3D structure databases

ProteinModelPortaliP9WH63.
SMRiP9WH63. Positions 2-118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S12P family.Curated

Phylogenomic databases

eggNOGiENOG4108UKE. Bacteria.
COG0048. LUCA.
KOiK02950.
OMAiTRYHIIR.
PhylomeDBiP9WH63.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00403_B. Ribosomal_S12_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005679. Ribosomal_S12_bac.
[Graphical view]
PANTHERiPTHR11652. PTHR11652. 1 hit.
PfamiPF00164. Ribosom_S12_S23. 1 hit.
[Graphical view]
PIRSFiPIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTSiPR01034. RIBOSOMALS12.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00981. rpsL_bact. 1 hit.
PROSITEiPS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WH63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTIQQLVRK GRRDKISKVK TAALKGSPQR RGVCTRVYTT TPKKPNSALR
60 70 80 90 100
KVARVKLTSQ VEVTAYIPGE GHNLQEHSMV LVRGGRVKDL PGVRYKIIRG
110 120
SLDTQGVKNR KQARSRYGAK KEKG
Length:124
Mass (Da):13,849
Last modified:April 16, 2014 - v1
Checksum:i2B441B040CEF20CD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti43 – 431K → R in strain: 9106, 9181, An03, F12, F18 and TCVGH21; resistant to streptomycin.
Natural varianti43 – 431K → T in strain: TCVGH25; probably resistant to streptomycin. 1 Publication
Natural varianti88 – 881K → Q in strain: F05; resistant to streptomycin.
Natural varianti88 – 881K → R in strain: C37; probably resistant to streptomycin.
Natural varianti88 – 881K → T in strain: F18; resistant to streptomycin.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70995 Genomic DNA. Translation: CAA50323.1.
L08011 Genomic DNA. Translation: AAA66176.1.
AL123456 Genomic DNA. Translation: CCP43425.1.
AF398879 Genomic DNA. Translation: AAK83385.1.
AF398880 Genomic DNA. Translation: AAK83386.1.
AF398881 Genomic DNA. Translation: AAK83387.1.
AF398882 Genomic DNA. Translation: AAK83388.1.
AY156729 Genomic DNA. Translation: AAN52761.1.
AY156730 Genomic DNA. Translation: AAN52762.1.
AY156732 Genomic DNA. Translation: AAN52764.1.
AY156733 Genomic DNA. Translation: AAN52765.1.
AF367438 Genomic DNA. Translation: AAK53753.1.
PIRiS39591.
RefSeqiNP_215196.1. NC_000962.3.
WP_003403453.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP43425; CCP43425; Rv0682.
GeneIDi888259.
KEGGimtu:Rv0682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70995 Genomic DNA. Translation: CAA50323.1.
L08011 Genomic DNA. Translation: AAA66176.1.
AL123456 Genomic DNA. Translation: CCP43425.1.
AF398879 Genomic DNA. Translation: AAK83385.1.
AF398880 Genomic DNA. Translation: AAK83386.1.
AF398881 Genomic DNA. Translation: AAK83387.1.
AF398882 Genomic DNA. Translation: AAK83388.1.
AY156729 Genomic DNA. Translation: AAN52761.1.
AY156730 Genomic DNA. Translation: AAN52762.1.
AY156732 Genomic DNA. Translation: AAN52764.1.
AY156733 Genomic DNA. Translation: AAN52765.1.
AF367438 Genomic DNA. Translation: AAK53753.1.
PIRiS39591.
RefSeqiNP_215196.1. NC_000962.3.
WP_003403453.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliP9WH63.
SMRiP9WH63. Positions 2-118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv0682.

Chemistry

ChEMBLiCHEMBL2363965.

Proteomic databases

PaxDbiP9WH63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP43425; CCP43425; Rv0682.
GeneIDi888259.
KEGGimtu:Rv0682.

Organism-specific databases

TubercuListiRv0682.

Phylogenomic databases

eggNOGiENOG4108UKE. Bacteria.
COG0048. LUCA.
KOiK02950.
OMAiTRYHIIR.
PhylomeDBiP9WH63.

Miscellaneous databases

PROiP9WH63.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00403_B. Ribosomal_S12_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005679. Ribosomal_S12_bac.
[Graphical view]
PANTHERiPTHR11652. PTHR11652. 1 hit.
PfamiPF00164. Ribosom_S12_S23. 1 hit.
[Graphical view]
PIRSFiPIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTSiPR01034. RIBOSOMALS12.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00981. rpsL_bact. 1 hit.
PROSITEiPS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular basis of streptomycin resistance in Mycobacterium tuberculosis: alterations of the ribosomal protein S12 gene and point mutations within a functional 16S ribosomal RNA pseudoknot."
    Finken M., Kirschner P., Meier A., Wrede A., Boettger E.C.
    Mol. Microbiol. 9:1239-1246(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS STREPTOMYCIN RESISTANT.
  2. "The rpsL gene and streptomycin resistance in single and multiple drug-resistant strains of Mycobacterium tuberculosis."
    Nair J., Rouse D.A., Bai G.H., Morris S.L.
    Mol. Microbiol. 10:521-527(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  5. "Mutations associated to streptomycin resistant strains of Mycobacterium tuberculosis isolated in Italy."
    Orru G., Iona E., Vicidomini S., Oggioni M.R., Fattorini L., Orefici G., Pozzi G.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: An03, F05, F12 and F18.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  7. "The allelic profiles of multidrug-resistant Mycobacterium tuberculosis isolates from Taiwan."
    Shi Z.-Y., Lee K., Hu C.-H., Liu M.-F.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: VARIANTS STREPTOMYCIN RESISTANT.
    Strain: C37, C9, TCVGH1, TCVGH21 and TCVGH25.
  8. Hainan H., Jinxiang H., Changzheng S.S.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-104, VARIANT THR-43.

Entry informationi

Entry nameiRS12_MYCTU
AccessioniPrimary (citable) accession number: P9WH63
Secondary accession number(s): L0T4E7
, P41196, Q933X1, Q93MR7, Q93MR8, Q93T44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: June 8, 2016
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Because the enzyme that would modify Asp-89 to 3-methylthioaspartic acid has not been found in the proteome of this organism, that modification is not predicted.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.