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Protein

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

Gene

fabG3

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Androstan-3-alpha,17-beta-diol + NAD+ = 17-beta-hydroxyandrostan-3-one + NADH.

Pathwayi: C21-steroid hormone metabolism

This protein is involved in the pathway C21-steroid hormone metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway C21-steroid hormone metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140Substrate1
Active sitei153Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 35NADBy similarityAdd BLAST25

GO - Molecular functioni

  • androstan-3-alpha,17-beta-diol dehydrogenase activity Source: MTBBASE

GO - Biological processi

  • C21-steroid hormone metabolic process Source: UniProtKB-UniPathway
  • steroid metabolic process Source: MTBBASE

Keywordsi

Molecular functionOxidoreductase
Biological processLipid metabolism, Steroid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00229.

Chemistry databases

SwissLipidsiSLP:000001178.

Names & Taxonomyi

Protein namesi
Recommended name:
3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase (EC:1.1.1.53)
Gene namesi
Name:fabG3
Ordered Locus Names:Rv2002
ORF Names:MTCY39.16c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2002.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: MTBBASE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000547121 – 2603-alpha-(or 20-beta)-hydroxysteroid dehydrogenaseAdd BLAST260

Proteomic databases

PaxDbiP9WGT1.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi83332.Rv2002.

Structurei

Secondary structure

1260
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni4 – 7Combined sources4
Beta strandi9 – 13Combined sources5
Turni14 – 16Combined sources3
Helixi18 – 29Combined sources12
Beta strandi33 – 39Combined sources7
Helixi41 – 50Combined sources10
Helixi52 – 54Combined sources3
Beta strandi55 – 59Combined sources5
Helixi65 – 79Combined sources15
Beta strandi84 – 87Combined sources4
Turni97 – 99Combined sources3
Helixi102 – 112Combined sources11
Helixi114 – 130Combined sources17
Beta strandi133 – 138Combined sources6
Helixi141 – 143Combined sources3
Helixi151 – 171Combined sources21
Helixi172 – 174Combined sources3
Beta strandi176 – 183Combined sources8
Helixi189 – 191Combined sources3
Beta strandi202 – 205Combined sources4
Helixi209 – 220Combined sources12
Helixi222 – 224Combined sources3
Beta strandi231 – 235Combined sources5
Helixi238 – 240Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NFFX-ray1.80A/B1-260[»]
1NFQX-ray2.40A/B/C/D1-260[»]
1NFRX-ray2.10A/B/C/D1-260[»]
ProteinModelPortaliP9WGT1.
SMRiP9WGT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00038.
OMAiQEWALPR.
PhylomeDBiP9WGT1.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WGT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGRLIGKVA LVSGGARGMG ASHVRAMVAE GAKVVFGDIL DEEGKAVAAE
60 70 80 90 100
LADAARYVHL DVTQPAQWTA AVDTAVTAFG GLHVLVNNAG ILNIGTIEDY
110 120 130 140 150
ALTEWQRILD VNLTGVFLGI RAVVKPMKEA GRGSIINISS IEGLAGTVAC
160 170 180 190 200
HGYTATKFAV RGLTKSTALE LGPSGIRVNS IHPGLVKTPM TDWVPEDIFQ
210 220 230 240 250
TALGRAAEPV EVSNLVVYLA SDESSYSTGA EFVVDGGTVA GLAHNDFGAV
260
EVSSQPEWVT
Length:260
Mass (Da):27,030
Last modified:April 16, 2014 - v1
Checksum:i0935A14ED36220B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44774.1.
PIRiH70758.
RefSeqiNP_216518.1. NC_000962.3.
WP_003410047.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44774; CCP44774; Rv2002.
GeneIDi888857.
KEGGimtu:Rv2002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44774.1.
PIRiH70758.
RefSeqiNP_216518.1. NC_000962.3.
WP_003410047.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NFFX-ray1.80A/B1-260[»]
1NFQX-ray2.40A/B/C/D1-260[»]
1NFRX-ray2.10A/B/C/D1-260[»]
ProteinModelPortaliP9WGT1.
SMRiP9WGT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2002.

Chemistry databases

SwissLipidsiSLP:000001178.

Proteomic databases

PaxDbiP9WGT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP44774; CCP44774; Rv2002.
GeneIDi888857.
KEGGimtu:Rv2002.

Organism-specific databases

TubercuListiRv2002.

Phylogenomic databases

KOiK00038.
OMAiQEWALPR.
PhylomeDBiP9WGT1.

Enzyme and pathway databases

UniPathwayiUPA00229.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHSD_MYCTU
AccessioniPrimary (citable) accession number: P9WGT1
Secondary accession number(s): L0TB81, P69167, Q10855
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: April 12, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.