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Protein

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

Gene

fabG3

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Androstan-3-alpha,17-beta-diol + NAD+ = 17-beta-hydroxyandrostan-3-one + NADH.

Pathwayi: C21-steroid hormone metabolism

This protein is involved in the pathway C21-steroid hormone metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway C21-steroid hormone metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei140 – 1401Substrate
Active sitei153 – 1531Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 3525NADBy similarityAdd
BLAST

GO - Molecular functioni

  • androstan-3-alpha,17-beta-diol dehydrogenase activity Source: MTBBASE

GO - Biological processi

  • C21-steroid hormone metabolic process Source: UniProtKB-UniPathway
  • steroid metabolic process Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00229.

Chemistry

SwissLipidsiSLP:000001178.

Names & Taxonomyi

Protein namesi
Recommended name:
3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase (EC:1.1.1.53)
Gene namesi
Name:fabG3
Ordered Locus Names:Rv2002
ORF Names:MTCY39.16c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2002.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2602603-alpha-(or 20-beta)-hydroxysteroid dehydrogenasePRO_0000054712Add
BLAST

Proteomic databases

PaxDbiP9WGT1.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi83332.Rv2002.

Structurei

Secondary structure

1
260
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni4 – 74Combined sources
Beta strandi9 – 135Combined sources
Turni14 – 163Combined sources
Helixi18 – 2912Combined sources
Beta strandi33 – 397Combined sources
Helixi41 – 5010Combined sources
Helixi52 – 543Combined sources
Beta strandi55 – 595Combined sources
Helixi65 – 7915Combined sources
Beta strandi84 – 874Combined sources
Turni97 – 993Combined sources
Helixi102 – 11211Combined sources
Helixi114 – 13017Combined sources
Beta strandi133 – 1386Combined sources
Helixi141 – 1433Combined sources
Helixi151 – 17121Combined sources
Helixi172 – 1743Combined sources
Beta strandi176 – 1838Combined sources
Helixi189 – 1913Combined sources
Beta strandi202 – 2054Combined sources
Helixi209 – 22012Combined sources
Helixi222 – 2243Combined sources
Beta strandi231 – 2355Combined sources
Helixi238 – 2403Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NFFX-ray1.80A/B1-260[»]
1NFQX-ray2.40A/B/C/D1-260[»]
1NFRX-ray2.10A/B/C/D1-260[»]
ProteinModelPortaliP9WGT1.
SMRiP9WGT1. Positions 2-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00038.
OMAiQEWALPR.
PhylomeDBiP9WGT1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WGT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGRLIGKVA LVSGGARGMG ASHVRAMVAE GAKVVFGDIL DEEGKAVAAE
60 70 80 90 100
LADAARYVHL DVTQPAQWTA AVDTAVTAFG GLHVLVNNAG ILNIGTIEDY
110 120 130 140 150
ALTEWQRILD VNLTGVFLGI RAVVKPMKEA GRGSIINISS IEGLAGTVAC
160 170 180 190 200
HGYTATKFAV RGLTKSTALE LGPSGIRVNS IHPGLVKTPM TDWVPEDIFQ
210 220 230 240 250
TALGRAAEPV EVSNLVVYLA SDESSYSTGA EFVVDGGTVA GLAHNDFGAV
260
EVSSQPEWVT
Length:260
Mass (Da):27,030
Last modified:April 16, 2014 - v1
Checksum:i0935A14ED36220B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44774.1.
PIRiH70758.
RefSeqiNP_216518.1. NC_000962.3.
WP_003410047.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44774; CCP44774; Rv2002.
GeneIDi888857.
KEGGimtu:Rv2002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44774.1.
PIRiH70758.
RefSeqiNP_216518.1. NC_000962.3.
WP_003410047.1. NZ_KK339370.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NFFX-ray1.80A/B1-260[»]
1NFQX-ray2.40A/B/C/D1-260[»]
1NFRX-ray2.10A/B/C/D1-260[»]
ProteinModelPortaliP9WGT1.
SMRiP9WGT1. Positions 2-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2002.

Chemistry

SwissLipidsiSLP:000001178.

Proteomic databases

PaxDbiP9WGT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP44774; CCP44774; Rv2002.
GeneIDi888857.
KEGGimtu:Rv2002.

Organism-specific databases

TubercuListiRv2002.

Phylogenomic databases

KOiK00038.
OMAiQEWALPR.
PhylomeDBiP9WGT1.

Enzyme and pathway databases

UniPathwayiUPA00229.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSD_MYCTU
AccessioniPrimary (citable) accession number: P9WGT1
Secondary accession number(s): L0TB81, P69167, Q10855
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: September 7, 2016
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.