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Protein

Protein translocase subunit SecA 1

Gene

secA1

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of precursor proteins, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi101 – 1088ATPUniRule annotation

GO - Molecular functioni

  • ATPase activity Source: MTBBASE
  • ATP binding Source: MTBBASE
  • protein-transmembrane transporting ATPase activity Source: GO_Central

GO - Biological processi

  • evasion or tolerance by symbiont of host-produced reactive oxygen species Source: Reactome
  • growth Source: MTBBASE
  • intracellular protein transmembrane transport Source: UniProtKB-HAMAP
  • protein import Source: InterPro
  • protein targeting Source: UniProtKB-HAMAP
  • protein transport by the Sec complex Source: MTBBASE
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1222387. Tolerance of reactive oxygen produced by macrophages.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecA 1UniRule annotation
Short name:
tbSecA
Gene namesi
Name:secA1UniRule annotation
Ordered Locus Names:Rv3240c
ORF Names:MTCY20B11.15c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3240c.

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Cytoplasm UniRule annotation

  • Note: Distribution is 50-50.UniRule annotation

GO - Cellular componenti

  • cell envelope Sec protein transport complex Source: MTBBASE
  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular region Source: MTBBASE
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 949949Protein translocase subunit SecA 1PRO_0000109598Add
BLAST

Proteomic databases

PaxDbiP9WGP5.

Interactioni

Subunit structurei

Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved (By similarity). Monomer and homodimer.By similarity

Protein-protein interaction databases

STRINGi83332.Rv3240c.

Structurei

Secondary structure

1
949
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 76Combined sources
Helixi12 – 2615Combined sources
Helixi28 – 325Combined sources
Helixi36 – 5116Combined sources
Beta strandi53 – 553Combined sources
Helixi59 – 7820Combined sources
Helixi84 – 9411Combined sources
Beta strandi97 – 1004Combined sources
Helixi107 – 1104Combined sources
Helixi112 – 1198Combined sources
Beta strandi125 – 1317Combined sources
Helixi132 – 14817Combined sources
Beta strandi153 – 1553Combined sources
Helixi162 – 1709Combined sources
Beta strandi171 – 1777Combined sources
Helixi178 – 18811Combined sources
Helixi194 – 1963Combined sources
Beta strandi204 – 2074Combined sources
Helixi210 – 2145Combined sources
Helixi217 – 2193Combined sources
Beta strandi222 – 2276Combined sources
Helixi232 – 24413Combined sources
Turni247 – 2493Combined sources
Beta strandi250 – 2545Combined sources
Turni255 – 2584Combined sources
Beta strandi259 – 2624Combined sources
Helixi264 – 27411Combined sources
Helixi287 – 30014Combined sources
Turni304 – 3063Combined sources
Beta strandi307 – 3093Combined sources
Beta strandi314 – 3174Combined sources
Beta strandi319 – 3213Combined sources
Helixi334 – 3418Combined sources
Beta strandi350 – 3567Combined sources
Helixi358 – 3625Combined sources
Beta strandi365 – 3739Combined sources
Helixi376 – 3783Combined sources
Helixi379 – 3868Combined sources
Beta strandi389 – 3924Combined sources
Beta strandi407 – 4104Combined sources
Helixi412 – 42817Combined sources
Beta strandi433 – 4386Combined sources
Helixi440 – 45213Combined sources
Beta strandi458 – 4603Combined sources
Helixi465 – 4739Combined sources
Turni474 – 4763Combined sources
Beta strandi481 – 4855Combined sources
Helixi500 – 51011Combined sources
Turni515 – 5173Combined sources
Helixi519 – 53618Combined sources
Helixi539 – 5468Combined sources
Beta strandi549 – 5546Combined sources
Helixi561 – 5699Combined sources
Helixi573 – 5753Combined sources
Beta strandi578 – 5858Combined sources
Helixi589 – 5935Combined sources
Helixi596 – 60510Combined sources
Helixi617 – 66347Combined sources
Helixi669 – 68618Combined sources
Beta strandi689 – 6913Combined sources
Helixi698 – 70811Combined sources
Turni715 – 7184Combined sources
Helixi736 – 75823Combined sources
Helixi762 – 79332Combined sources
Helixi794 – 7963Combined sources
Helixi803 – 83230Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NKTX-ray2.60A/B2-892[»]
1NL3X-ray2.80A/B2-892[»]
ProteinModelPortaliP9WGP5.
SMRiP9WGP5. Positions 1-835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
KOiK03070.
OMAiIATERHE.
PhylomeDBiP9WGP5.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 3 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 1 hit.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WGP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSKLLRLGE GRMVKRLKKV ADYVGTLSDD VEKLTDAELR AKTDEFKRRL
60 70 80 90 100
ADQKNPETLD DLLPEAFAVA REAAWRVLDQ RPFDVQVMGA AALHLGNVAE
110 120 130 140 150
MKTGEGKTLT CVLPAYLNAL AGNGVHIVTV NDYLAKRDSE WMGRVHRFLG
160 170 180 190 200
LQVGVILATM TPDERRVAYN ADITYGTNNE FGFDYLRDNM AHSLDDLVQR
210 220 230 240 250
GHHYAIVDEV DSILIDEART PLIISGPADG ASNWYTEFAR LAPLMEKDVH
260 270 280 290 300
YEVDLRKRTV GVHEKGVEFV EDQLGIDNLY EAANSPLVSY LNNALKAKEL
310 320 330 340 350
FSRDKDYIVR DGEVLIVDEF TGRVLIGRRY NEGMHQAIEA KEHVEIKAEN
360 370 380 390 400
QTLATITLQN YFRLYDKLAG MTGTAQTEAA ELHEIYKLGV VSIPTNMPMI
410 420 430 440 450
REDQSDLIYK TEEAKYIAVV DDVAERYAKG QPVLIGTTSV ERSEYLSRQF
460 470 480 490 500
TKRRIPHNVL NAKYHEQEAT IIAVAGRRGG VTVATNMAGR GTDIVLGGNV
510 520 530 540 550
DFLTDQRLRE RGLDPVETPE EYEAAWHSEL PIVKEEASKE AKEVIEAGGL
560 570 580 590 600
YVLGTERHES RRIDNQLRGR SGRQGDPGES RFYLSLGDEL MRRFNGAALE
610 620 630 640 650
TLLTRLNLPD DVPIEAKMVT RAIKSAQTQV EQQNFEVRKN VLKYDEVMNQ
660 670 680 690 700
QRKVIYAERR RILEGENLKD QALDMVRDVI TAYVDGATGE GYAEDWDLDA
710 720 730 740 750
LWTALKTLYP VGITADSLTR KDHEFERDDL TREELLEALL KDAERAYAAR
760 770 780 790 800
EAELEEIAGE GAMRQLERNV LLNVIDRKWR EHLYEMDYLK EGIGLRAMAQ
810 820 830 840 850
RDPLVEYQRE GYDMFMAMLD GMKEESVGFL FNVTVEAVPA PPVAPAAEPA
860 870 880 890 900
ELAEFAAAAA AAAQQRSAVD GGARERAPSA LRAKGVASES PALTYSGPAE
910 920 930 940
DGSAQVQRNG GGAHKTPAGV PAGASRRERR EAARRQGRGA KPPKSVKKR
Length:949
Mass (Da):106,022
Last modified:April 16, 2014 - v1
Checksum:iD46C3315835E03D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46059.1.
PIRiB70592.
RefSeqiWP_003901576.1. NZ_KK339370.1.
YP_177950.1. NC_000962.3.

Genome annotation databases

EnsemblBacteriaiCCP46059; CCP46059; Rv3240c.
GeneIDi888860.
KEGGimtu:Rv3240c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46059.1.
PIRiB70592.
RefSeqiWP_003901576.1. NZ_KK339370.1.
YP_177950.1. NC_000962.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NKTX-ray2.60A/B2-892[»]
1NL3X-ray2.80A/B2-892[»]
ProteinModelPortaliP9WGP5.
SMRiP9WGP5. Positions 1-835.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3240c.

Proteomic databases

PaxDbiP9WGP5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46059; CCP46059; Rv3240c.
GeneIDi888860.
KEGGimtu:Rv3240c.

Organism-specific databases

TubercuListiRv3240c.

Phylogenomic databases

eggNOGiENOG4105CI6. Bacteria.
COG0653. LUCA.
KOiK03070.
OMAiIATERHE.
PhylomeDBiP9WGP5.

Enzyme and pathway databases

ReactomeiR-HSA-1222387. Tolerance of reactive oxygen produced by macrophages.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 3 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 1 hit.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSECA1_MYCTU
AccessioniPrimary (citable) accession number: P9WGP5
Secondary accession number(s): L0TF13
, O05885, P0A5Y8, P71494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: July 6, 2016
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.