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Protein

Redox sensor histidine kinase response regulator DevS

Gene

devS

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Member of the two-component regulatory system DevR/DevS (DosR/DosS) involved in onset of the dormancy response. May act as a redox sensor (rather than a direct hypoxia sensor); the normal (aerobic growth) state is the Fe3+ form, while the reduced (anaerobic growth) Fe2+ form is probably active for phosphate transfer. It is probably reduced by flavin nucleotides such as FMN and FAD. May be the primary sensor for CO. Donates a phosphate group to DevR (DosR) (By similarity).By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • hemeBy similarityNote: Binds 1 heme group per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi149 – 1491Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

Heme, Iron, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Redox sensor histidine kinase response regulator DevS (EC:2.7.13.3)
Gene namesi
Name:devS
Synonyms:dosS
Ordered Locus Names:MT3218
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001020 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Redox sensor histidine kinase response regulator DevSPRO_0000428347Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei395 – 3951Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

A member of the dormancy regulon. Induced in response to reduced oxygen tension (hypoxia) and low levels of nitric oxide (NO).1 Publication

Structurei

3D structure databases

ProteinModelPortaliP9WGK2.
SMRiP9WGK2. Positions 63-207, 231-578.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 200138GAF 1Add
BLAST
Domaini231 – 369139GAF 2Add
BLAST
Domaini383 – 578196Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 GAF domains.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK07682.
OrthoDBiPOG091H03PP.

Family and domain databases

Gene3Di3.30.450.40. 2 hits.
3.30.565.10. 2 hits.
InterProiIPR027035. DosT/DevS.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PANTHERiPTHR11347:SF114. PTHR11347:SF114. 2 hits.
PfamiPF13185. GAF_2. 2 hits.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
SMARTiSM00065. GAF. 2 hits.
SM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 2 hits.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WGK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTGGLVDEN DGAAMRPLRH TLSQLRLHEL LVEVQDRVEQ IVEGRDRLDG
60 70 80 90 100
LVEAMLVVTA GLDLEATLRA IVHSATSLVD ARYGAMEVHD RQHRVLHFVY
110 120 130 140 150
EGIDEETVRR IGHLPKGLGV IGLLIEDPKP LRLDDVSAHP ASIGFPPYHP
160 170 180 190 200
PMRTFLGVPV RVRDESFGTL YLTDKTNGQP FSDDDEVLVQ ALAAAAGIAV
210 220 230 240 250
ANARLYQQAK ARQSWIEATR DIATELLSGT EPATVFRLVA AEALKLTAAD
260 270 280 290 300
AALVAVPVDE DMPAADVGEL LVIETVGSAV ASIVGRTIPV AGAVLREVFV
310 320 330 340 350
NGIPRRVDRV DLEGLDELAD AGPALLLPLR ARGTVAGVVV VLSQGGPGAF
360 370 380 390 400
TDEQLEMMAA FADQAALAWQ LATSQRRMRE LDVLTDRDRI ARDLHDHVIQ
410 420 430 440 450
RLFAIGLALQ GAVPHERNPE VQQRLSDVVD DLQDVIQEIR TTIYDLHGAS
460 470 480 490 500
QGITRLRQRI DAAVAQFADS GLRTSVQFVG PLSVVDSALA DQAEAVVREA
510 520 530 540 550
VSNAVRHAKA STLTVRVKVD DDLCIEVTDN GRGLPDEFTG SGLTNLRQRA
560 570
EQAGGEFTLA SVPGASGTVL RWSAPLSQ
Length:578
Mass (Da):62,241
Last modified:April 16, 2014 - v1
Checksum:i4C04B836791B9B32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK47556.1.
PIRiE70645.
RefSeqiWP_003899933.1. NZ_KK341227.1.

Genome annotation databases

EnsemblBacteriaiAAK47556; AAK47556; MT3218.
KEGGimtc:MT3218.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA. Translation: AAK47556.1.
PIRiE70645.
RefSeqiWP_003899933.1. NZ_KK341227.1.

3D structure databases

ProteinModelPortaliP9WGK2.
SMRiP9WGK2. Positions 63-207, 231-578.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK47556; AAK47556; MT3218.
KEGGimtc:MT3218.

Phylogenomic databases

KOiK07682.
OrthoDBiPOG091H03PP.

Family and domain databases

Gene3Di3.30.450.40. 2 hits.
3.30.565.10. 2 hits.
InterProiIPR027035. DosT/DevS.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
[Graphical view]
PANTHERiPTHR11347:SF114. PTHR11347:SF114. 2 hits.
PfamiPF13185. GAF_2. 2 hits.
PF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
SMARTiSM00065. GAF. 2 hits.
SM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 2 hits.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEVS_MYCTO
AccessioniPrimary (citable) accession number: P9WGK2
Secondary accession number(s): L0TBM4
, P95194, Q79CX7, Q7D626
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: September 7, 2016
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.