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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi33ZincBy similarity1
Metal bindingi211ZincBy similarity1
Metal bindingi236ZincBy similarity1
Metal bindingi240ZincBy similarity1
Binding sitei270ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cysteinyl-tRNA aminoacylation Source: GO_Central
  • growth Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-7858-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Synonyms:cysS1
Ordered Locus Names:Rv3580c
ORF Names:MTCY06G11.27c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3580c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001594411 – 469Cysteine--tRNA ligaseAdd BLAST469

Proteomic databases

PaxDbiP9WFW1.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi83332.Rv3580c.

Structurei

3D structure databases

ProteinModelPortaliP9WFW1.
SMRiP9WFW1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 45"HIGH" regionAdd BLAST11
Motifi267 – 271"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
KOiK01883.
OMAiYQIDIHA.
PhylomeDBiP9WFW1.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

P9WFW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRARLRLH DTAAGVVRDF VPLRPGHVSI YLCGATVQGL PHIGHVRSGV
60 70 80 90 100
AFDILRRWLL ARGYDVAFIR NVTDIEDKIL AKAAAAGRPW WEWAATHERA
110 120 130 140 150
FTAAYDALDV LPPSAEPRAT GHITQMIEMI ERLIQAGHAY TGGGDVYFDV
160 170 180 190 200
LSYPEYGQLS GHKIDDVHQG EGVAAGKRDQ RDFTLWKGEK PGEPSWPTPW
210 220 230 240 250
GRGRPGWHLE CSAMARSYLG PEFDIHCGGM DLVFPHHENE IAQSRAAGDG
260 270 280 290 300
FARYWLHNGW VTMGGEKMSK SLGNVLSMPA MLQRVRPAEL RYYLGSAHYR
310 320 330 340 350
SMLEFSETAM QDAVKAYVGL EDFLHRVRTR VGAVCPGDPT PRFAEALDDD
360 370 380 390 400
LSVPIALAEI HHVRAEGNRA LDAGDHDGAL RSASAIRAMM GILGCDPLDQ
410 420 430 440 450
RWESRDETSA ALAAVDVLVQ AELQNREKAR EQRNWALADE IRGRLKRAGI
460
EVTDTADGPQ WSLLGGDTK
Length:469
Mass (Da):51,855
Last modified:April 16, 2014 - v1
Checksum:i515F9D19482ADC0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46403.1.
PIRiB70607.
RefSeqiWP_003900108.1. NZ_KK339374.1.
YP_177992.1. NC_000962.3.

Genome annotation databases

EnsemblBacteriaiCCP46403; CCP46403; Rv3580c.
GeneIDi888628.
KEGGimtu:Rv3580c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46403.1.
PIRiB70607.
RefSeqiWP_003900108.1. NZ_KK339374.1.
YP_177992.1. NC_000962.3.

3D structure databases

ProteinModelPortaliP9WFW1.
SMRiP9WFW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv3580c.

Proteomic databases

PaxDbiP9WFW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46403; CCP46403; Rv3580c.
GeneIDi888628.
KEGGimtu:Rv3580c.

Organism-specific databases

TubercuListiRv3580c.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
KOiK01883.
OMAiYQIDIHA.
PhylomeDBiP9WFW1.

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-7858-MONOMER.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYC_MYCTU
AccessioniPrimary (citable) accession number: P9WFW1
Secondary accession number(s): L0TCZ2, P0A634, P96862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.