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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei742ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • growth Source: MTBBASE
  • leucyl-tRNA aminoacylation Source: GO_Central

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Rv0041
ORF Names:MTCY21D4.04
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0041.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: GO_Central
  • plasma membrane Source: MTBBASE

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001520522 – 969Leucine--tRNA ligaseAdd BLAST968

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP9WFV1.

PTM databases

iPTMnetiP9WFV1.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0041.

Structurei

Secondary structure

1969
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi314 – 320Combined sources7
Beta strandi331 – 337Combined sources7
Helixi339 – 344Combined sources6
Beta strandi347 – 350Combined sources4
Helixi357 – 360Combined sources4
Helixi371 – 373Combined sources3
Turni374 – 376Combined sources3
Helixi380 – 391Combined sources12
Helixi396 – 399Combined sources4
Beta strandi408 – 415Combined sources8
Turni417 – 419Combined sources3
Beta strandi422 – 427Combined sources6
Beta strandi438 – 442Combined sources5
Turni444 – 446Combined sources3
Helixi448 – 457Combined sources10
Turni470 – 472Combined sources3
Helixi485 – 487Combined sources3
Helixi492 – 505Combined sources14
Beta strandi508 – 511Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AGRX-ray1.30A309-513[»]
5AGSX-ray1.47A309-513[»]
5AGTX-ray1.45A309-513[»]
ProteinModelPortaliP9WFV1.
SMRiP9WFV1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 89"HIGH" regionAdd BLAST12
Motifi739 – 743"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
KOiK01869.
OMAiTFMVLAP.
PhylomeDBiP9WFV1.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
PANTHERiPTHR43740. PTHR43740. 1 hit.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WFV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESPTAGPG GVPRADDADS DVPRYRYTAE LAARLERTWQ ENWARLGTFN
60 70 80 90 100
VPNPVGSLAP PDGAAVPDDK LFVQDMFPYP SGEGLHVGHP LGYIATDVYA
110 120 130 140 150
RYFRMVGRNV LHALGFDAFG LPAEQYAVQT GTHPRTRTEA NVVNFRRQLG
160 170 180 190 200
RLGFGHDSRR SFSTTDVDFY RWTQWIFLQI YNAWFDTTAN KARPISELVA
210 220 230 240 250
EFESGARCLD GGRDWAKLTA GERADVIDEY RLVYRADSLV NWCPGLGTVL
260 270 280 290 300
ANEEVTADGR SDRGNFPVFR KRLRQWMMRI TAYADRLLDD LDVLDWPEQV
310 320 330 340 350
KTMQRNWIGR STGAVALFSA RAASDDGFEV DIEVFTTRPD TLFGATYLVL
360 370 380 390 400
APEHDLVDEL VAASWPAGVN PLWTYGGGTP GEAIAAYRRA IAAKSDLERQ
410 420 430 440 450
ESREKTGVFL GSYAINPANG EPVPIFIADY VLAGYGTGAI MAVPGHDQRD
460 470 480 490 500
WDFARAFGLP IVEVIAGGNI SESAYTGDGI LVNSDYLNGM SVPAAKRAIV
510 520 530 540 550
DRLESAGRGR ARIEFKLRDW LFARQRYWGE PFPIVYDSDG RPHALDEAAL
560 570 580 590 600
PVELPDVPDY SPVLFDPDDA DSEPSPPLAK ATEWVHVDLD LGDGLKPYSR
610 620 630 640 650
DTNVMPQWAG SSWYELRYTD PHNSERFCAK ENEAYWMGPR PAEHGPDDPG
660 670 680 690 700
GVDLYVGGAE HAVLHLLYSR FWHKVLYDLG HVSSREPYRR LVNQGYIQAY
710 720 730 740 750
AYTDARGSYV PAEQVIERGD RFVYPGPDGE VEVFQEFGKI GKSLKNSVSP
760 770 780 790 800
DEICDAYGAD TLRVYEMSMG PLEASRPWAT KDVVGAYRFL QRVWRLVVDE
810 820 830 840 850
HTGETRVADG VELDIDTLRA LHRTIVGVSE DFAALRNNTA TAKLIEYTNH
860 870 880 890 900
LTKKHRDAVP RAAVEPLVQM LAPLAPHIAE ELWLRLGNTT SLAHGPFPKA
910 920 930 940 950
DAAYLVDETV EYPVQVNGKV RGRVVVAADT DEETLKAAVL TDEKVQAFLA
960
GATPRKVIVV AGRLVNLVI
Length:969
Mass (Da):107,595
Last modified:April 16, 2014 - v1
Checksum:iA07C835FC1B428D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP42763.1.
PIRiA70912.
RefSeqiNP_214555.1. NC_000962.3.
WP_003900794.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP42763; CCP42763; Rv0041.
GeneIDi887040.
KEGGimtu:Rv0041.

Similar proteinsi

Entry informationi

Entry nameiSYL_MYCTU
AccessioniPrimary (citable) accession number: P9WFV1
Secondary accession number(s): L0T451, P67510, P71698
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: January 31, 2018
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families