Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate synthase

Gene

thyA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei146 – 1461UniRule annotation

GO - Molecular functioni

  • thymidylate synthase activity Source: MTBBASE

GO - Biological processi

  • dTMP biosynthetic process Source: MTBBASE
  • dTTP biosynthetic process Source: UniProtKB-UniPathway
  • dUMP catabolic process Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Rv2764c
ORF Names:MTV002.29c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2764c.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1795162.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Thymidylate synthasePRO_0000140990Add
BLAST

Proteomic databases

PaxDbiP9WFR9.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi83332.Rv2764c.

Chemistry

BindingDBiP9WFR9.

Structurei

Secondary structure

1
263
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Beta strandi16 – 183Combined sources
Beta strandi21 – 233Combined sources
Beta strandi26 – 3712Combined sources
Helixi38 – 403Combined sources
Turni46 – 494Combined sources
Helixi52 – 6413Combined sources
Helixi70 – 745Combined sources
Helixi81 – 833Combined sources
Helixi94 – 1007Combined sources
Helixi111 – 12111Combined sources
Beta strandi129 – 1313Combined sources
Turni135 – 1373Combined sources
Helixi138 – 1403Combined sources
Beta strandi141 – 1433Combined sources
Beta strandi146 – 15510Combined sources
Beta strandi158 – 16912Combined sources
Turni170 – 1723Combined sources
Helixi173 – 19220Combined sources
Beta strandi195 – 20915Combined sources
Helixi210 – 2123Combined sources
Helixi213 – 2208Combined sources
Beta strandi229 – 2324Combined sources
Helixi238 – 2403Combined sources
Helixi243 – 2453Combined sources
Beta strandi246 – 2505Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QJ7X-ray2.50A/B/C/D1-263[»]
4FOAX-ray2.25A/B/C/D1-263[»]
4FOGX-ray2.40A/B/C/D1-263[»]
4FOXX-ray2.30A/B/C/D/E/F/G/H1-263[»]
4FQSX-ray1.80A/B1-263[»]
ProteinModelPortaliP9WFR9.
SMRiP9WFR9. Positions 1-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
KOiK00560.
OMAiIVYELLW.
PhylomeDBiP9WFR9.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P9WFR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPYEDLLRF VLETGTPKSD RTGTGTRSLF GQQMRYDLSA GFPLLTTKKV
60 70 80 90 100
HFKSVAYELL WFLRGDSNIG WLHEHGVTIW DEWASDTGEL GPIYGVQWRS
110 120 130 140 150
WPAPSGEHID QISAALDLLR TDPDSRRIIV SAWNVGEIER MALPPCHAFF
160 170 180 190 200
QFYVADGRLS CQLYQRSADL FLGVPFNIAS YALLTHMMAA QAGLSVGEFI
210 220 230 240 250
WTGGDCHIYD NHVEQVRLQL SREPRPYPKL LLADRDSIFE YTYEDIVVKN
260
YDPHPAIKAP VAV
Length:263
Mass (Da):29,853
Last modified:April 16, 2014 - v1
Checksum:iDF48279E3E9EA5F3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45563.1.
PIRiC70881.
RefSeqiNP_217280.1. NC_000962.3.
WP_003414075.1. NC_000962.3.

Genome annotation databases

EnsemblBacteriaiCCP45563; CCP45563; Rv2764c.
GeneIDi887728.
KEGGimtu:Rv2764c.
mtv:RVBD_2764c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP45563.1.
PIRiC70881.
RefSeqiNP_217280.1. NC_000962.3.
WP_003414075.1. NC_000962.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QJ7X-ray2.50A/B/C/D1-263[»]
4FOAX-ray2.25A/B/C/D1-263[»]
4FOGX-ray2.40A/B/C/D1-263[»]
4FOXX-ray2.30A/B/C/D/E/F/G/H1-263[»]
4FQSX-ray1.80A/B1-263[»]
ProteinModelPortaliP9WFR9.
SMRiP9WFR9. Positions 1-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2764c.

Chemistry

BindingDBiP9WFR9.
ChEMBLiCHEMBL1795162.

Proteomic databases

PaxDbiP9WFR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45563; CCP45563; Rv2764c.
GeneIDi887728.
KEGGimtu:Rv2764c.
mtv:RVBD_2764c.

Organism-specific databases

TubercuListiRv2764c.

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
KOiK00560.
OMAiIVYELLW.
PhylomeDBiP9WFR9.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_MYCTU
AccessioniPrimary (citable) accession number: P9WFR9
Secondary accession number(s): L0TD99, O33306, P67044
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: December 9, 2015
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.