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Protein

Urease subunit alpha

Gene

ureC

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation1 Publication

Cofactori

Ni cationUniRule annotation1 PublicationNote: Binds 2 nickel ions per subunit.UniRule annotation1 Publication

Kineticsi

  1. KM=0.3 mM for urea1 Publication
  1. Vmax=0.05 mmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.2.1 Publication

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit beta (ureB), Urease subunit gamma (ureA), Urease subunit gamma (ureA), Urease subunit alpha (ureC), Urease subunit alpha (ureC), Urease subunit beta (ureB)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi141Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi143Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi224Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi224Nickel 2; via carbamate groupUniRule annotation1
Binding sitei226SubstrateUniRule annotation1
Metal bindingi253Nickel 2; via pros nitrogen1
Metal bindingi279Nickel 2; via tele nitrogenUniRule annotation1
Active sitei327Proton donorUniRule annotation1
Metal bindingi367Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • growth Source: MTBBASE
  • urea catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-6045-MONOMER.
UniPathwayiUPA00258; UER00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:Rv1850
ORF Names:MTCY359.23c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1850.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000675492 – 577Urease subunit alphaAdd BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PaxDbiP9WFF1.

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.

Protein-protein interaction databases

STRINGi83332.Rv1850.

Structurei

3D structure databases

ProteinModelPortaliP9WFF1.
SMRiP9WFF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 577UreaseUniRule annotationAdd BLAST442

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
KOiK01428.
OMAiFDSHIHF.
PhylomeDBiP9WFF1.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WFF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLSRERYA QLYGPTTGDR IRLADTNLLV EVTEDRCGGP GLAGDEAVFG
60 70 80 90 100
GGKVLRESMG QGRASRADGA PDTVITGAVI IDYWGIIKAD IGIRDGRIVG
110 120 130 140 150
IGKAGNPDIM TGVHRDLVVG PSTEIISGNR RIVTAGTVDC HVHLICPQII
160 170 180 190 200
VEALAAGTTT IIGGGTGPAE GTKATTVTPG EWHLARMLES LDGWPVNFAL
210 220 230 240 250
LGKGNTVNPD ALWEQLRGGA SGFKLHEDWG STPAAIDTCL AVADVAGVQV
260 270 280 290 300
ALHSDTLNET GFVEDTIGAI AGRSIHAYHT EGAGGGHAPD IITVAAQPNV
310 320 330 340 350
LPSSTNPTRP HTVNTLDEHL DMLMVCHHLN PRIPEDLAFA ESRIRPSTIA
360 370 380 390 400
AEDVLHDMGA ISMIGSDSQA MGRVGEVVLR TWQTAHVMKA RRGALEGDPS
410 420 430 440 450
GSQAADNNRV RRYIAKYTIC PAIAHGMDHL IGSVEVGKLA DLVLWEPAFF
460 470 480 490 500
GVRPHVVLKG GAIAWAAMGD ANASIPTPQP VLPRPMFGAA AATAAATSVH
510 520 530 540 550
FVAPQSIDAR LADRLAVNRG LAPVADVRAV GKTDLPLNDA LPSIEVDPDT
560 570
FTVRIDGQVW QPQPAAELPM TQRYFLF
Length:577
Mass (Da):60,825
Last modified:April 16, 2014 - v1
Checksum:i010FB33C3FDFED12
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62G → A in AAC37007 (PubMed:7568014).Curated1
Sequence conflicti67A → T in AAC37007 (PubMed:7568014).Curated1
Sequence conflicti89A → T in AAC37007 (PubMed:7568014).Curated1
Sequence conflicti96 – 97GR → AP in AAC37007 (PubMed:7568014).Curated2
Sequence conflicti104A → T in AAC37007 (PubMed:7568014).Curated1
Sequence conflicti153A → T in AAC37007 (PubMed:7568014).Curated1
Sequence conflicti174A → T in AAC37007 (PubMed:7568014).Curated1
Sequence conflicti372G → D in AAC37007 (PubMed:7568014).Curated1
Sequence conflicti448A → V in AAC37007 (PubMed:7568014).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33011 Genomic DNA. Translation: AAC43475.1.
L41141 Genomic DNA. Translation: AAC37007.1.
AL123456 Genomic DNA. Translation: CCP44616.1.
PIRiB70665.
RefSeqiNP_216366.1. NC_000962.3.
WP_003899049.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44616; CCP44616; Rv1850.
GeneIDi885359.
KEGGimtu:Rv1850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33011 Genomic DNA. Translation: AAC43475.1.
L41141 Genomic DNA. Translation: AAC37007.1.
AL123456 Genomic DNA. Translation: CCP44616.1.
PIRiB70665.
RefSeqiNP_216366.1. NC_000962.3.
WP_003899049.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliP9WFF1.
SMRiP9WFF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1850.

Proteomic databases

PaxDbiP9WFF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP44616; CCP44616; Rv1850.
GeneIDi885359.
KEGGimtu:Rv1850.

Organism-specific databases

TubercuListiRv1850.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
KOiK01428.
OMAiFDSHIHF.
PhylomeDBiP9WFF1.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciMTBH37RV:G185E-6045-MONOMER.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURE1_MYCTU
AccessioniPrimary (citable) accession number: P9WFF1
Secondary accession number(s): L0T9F6, P0A660, P50042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: November 2, 2016
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Was identified as a high-confidence drug target.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.