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Protein

Isopentenyl-diphosphate delta-isomerase

Gene

fni

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).UniRule annotation

Catalytic activityi

Isopentenyl diphosphate = dimethylallyl diphosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FMNUniRule annotation
  • NADPHUniRule annotation
  • Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95FMN; via amide nitrogenUniRule annotation1
Binding sitei124FMNUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Metal bindingi155MagnesiumUniRule annotation1
Binding sitei186FMNUniRule annotation1
Binding sitei211FMNUniRule annotation1
Binding sitei216FMN; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi65 – 67FMNUniRule annotation3
Nucleotide bindingi262 – 264FMNUniRule annotation3
Nucleotide bindingi283 – 284FMNUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processIsoprene biosynthesis
LigandFlavoprotein, FMN, Magnesium, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isopentenyl-diphosphate delta-isomeraseUniRule annotation (EC:5.3.3.2UniRule annotation)
Short name:
IPP isomeraseUniRule annotation
Alternative name(s):
Isopentenyl diphosphate:dimethylallyl diphosphate isomeraseUniRule annotation
Isopentenyl pyrophosphate isomeraseUniRule annotation
Type 2 isopentenyl diphosphate isomeraseUniRule annotation
Short name:
IDI-2UniRule annotation
Gene namesi
Name:fniUniRule annotation
Ordered Locus Names:SA2136
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001344221 – 349Isopentenyl-diphosphate delta-isomeraseAdd BLAST349

2D gel databases

SWISS-2DPAGEiP99172

Interactioni

Subunit structurei

Homooctamer. Dimer of tetramers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP99172
SMRiP99172
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 10Substrate bindingUniRule annotation2
Regioni95 – 97Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the IPP isomerase type 2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000072127
KOiK01823
OMAiWDWGIPT

Family and domain databases

CDDicd02811 IDI-2_FMN, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00354 Idi_2, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000262 FMN-dep_DH
IPR011179 IPdP_isomerase
PANTHERiPTHR43665 PTHR43665, 1 hit
PfamiView protein in Pfam
PF01070 FMN_dh, 1 hit
PIRSFiPIRSF003314 IPP_isomerase, 1 hit
TIGRFAMsiTIGR02151 IPP_isom_2, 1 hit

Sequencei

Sequence statusi: Complete.

P99172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDFQREQRK NEHVEIAMAQ SDAMHSDFDK MRFVHHSIPS INVNDIDLTS
60 70 80 90 100
QTPDLTMAYP IYINAMTGGS EWTKNINEKL AVVARETRLA MAVGSTHAAL
110 120 130 140 150
RNPRMAETFT IARKMNPEGM IFSNVGADVP VEKALEAVEL LEAQALQIHV
160 170 180 190 200
NSPQELVMPE GNREFVTWLD NIASIVSRVS VPVIIKEVGF GMSKELMHDL
210 220 230 240 250
QQIGVKYVDV SGKGGTNFVD IENERRANKD MDYLSSWGQS TVESLLETTA
260 270 280 290 300
YQSEISVFAS GGLRTPLDAI KSLALGAKAT GMSRPFLNQV ENNGIAHTVA
310 320 330 340
YVESFIEHMK SIMTMLDAKN IDDLTQKQIV FSPEIMSWIE QRSLNIHRG
Length:349
Mass (Da):38,874
Last modified:October 11, 2004 - v1
Checksum:i9A13C4DA4188AF00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA Translation: BAB43438.1
PIRiE90034
RefSeqiWP_001279375.1, NC_002745.2

Genome annotation databases

EnsemblBacteriaiBAB43438; BAB43438; BAB43438
KEGGisau:SA2136

Similar proteinsi

Entry informationi

Entry nameiIDI2_STAAN
AccessioniPrimary (citable) accession number: P99172
Secondary accession number(s): Q99RS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health