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P99165

- PFKA_STAAN

UniProt

P99165 - PFKA_STAAN

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
    Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
    Active sitei129 – 1291Proton acceptorUniRule annotation
    Binding sitei156 – 1561Allosteric activator ADPUniRule annotation
    Binding sitei164 – 1641Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei213 – 2131Allosteric activator ADPUniRule annotation
    Binding sitei224 – 2241SubstrateUniRule annotation
    Binding sitei245 – 2451Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 732ATPUniRule annotation
    Nucleotide bindingi102 – 1054ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSAUR158879:GJCB-1594-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Synonyms:pfk
    Ordered Locus Names:SA1521
    OrganismiStaphylococcus aureus (strain N315)
    Taxonomic identifieri158879 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000751: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 322322ATP-dependent 6-phosphofructokinasePRO_0000111978Add
    BLAST

    2D gel databases

    SWISS-2DPAGEP99165.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi158879.SA1521.

    Structurei

    3D structure databases

    ProteinModelPortaliP99165.
    SMRiP99165. Positions 1-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni127 – 1293Substrate bindingUniRule annotation
    Regioni171 – 1733Substrate bindingUniRule annotation
    Regioni187 – 1893Allosteric activator ADP bindingUniRule annotation
    Regioni215 – 2173Allosteric activator ADP bindingUniRule annotation
    Regioni251 – 2544Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248870.
    KOiK00850.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P99165-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKIAVLTSG GDSPGMNAAV RAVVRTAIYN EIEVYGVYHG YQGLLNDDIH    50
    KLELGSVGDT IQRGGTFLYS ARCPEFKEQE VRKVAIENLR KRGIEGLVVI 100
    GGDGSYRGAQ RISEECKEIQ TIGIPGTIDN DINGTDFTIG FDTALNTIIG 150
    LVDKIRDTAS SHARTFIIEA MGRDCGDLAL WAGLSVGAET IVVPEVKTDI 200
    KEIADKIEQG IKRGKKHSIV LVAEGCMTAQ DCQKELSQYI NVDNRVSVLG 250
    HVQRGGSPTG ADRVLASRLG GYAVDLLMQG ETAKGVGIKN NKIVATSFDE 300
    IFDGKDHKFD YSLYELANKL SI 322
    Length:322
    Mass (Da):34,840
    Last modified:October 11, 2004 - v1
    Checksum:i1FAE41C0BA7CA7F1
    GO

    Sequence cautioni

    The sequence BAB42788.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000018 Genomic DNA. Translation: BAB42788.1. Different initiation.
    PIRiG89953.
    RefSeqiNP_374809.1. NC_002745.2.

    Genome annotation databases

    EnsemblBacteriaiBAB42788; BAB42788; BAB42788.
    GeneIDi1124366.
    KEGGisau:SA1521.
    PATRICi19575342. VBIStaAur116463_1641.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000018 Genomic DNA. Translation: BAB42788.1 . Different initiation.
    PIRi G89953.
    RefSeqi NP_374809.1. NC_002745.2.

    3D structure databases

    ProteinModelPortali P99165.
    SMRi P99165. Positions 1-322.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158879.SA1521.

    2D gel databases

    SWISS-2DPAGE P99165.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB42788 ; BAB42788 ; BAB42788 .
    GeneIDi 1124366.
    KEGGi sau:SA1521.
    PATRICi 19575342. VBIStaAur116463_1641.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248870.
    KOi K00850.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci SAUR158879:GJCB-1594-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: N315.
    2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: N315.
    3. "Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315."
      Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.
      Submitted (OCT-2007) to UniProtKB
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: N315.

    Entry informationi

    Entry nameiPFKA_STAAN
    AccessioniPrimary (citable) accession number: P99165
    Secondary accession number(s): Q99TG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3