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P99165

- PFKA_STAAN

UniProt

P99165 - PFKA_STAAN

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, pfk, SA1521
Organism
Staphylococcus aureus (strain N315)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarity
Metal bindingi103 – 1031Magnesium; catalytic By similarity
Active sitei129 – 1291Proton acceptor By similarity
Binding sitei156 – 1561Allosteric activator ADP By similarity
Binding sitei164 – 1641Substrate; shared with dimeric partner By similarity
Binding sitei213 – 2131Allosteric activator ADP By similarity
Binding sitei224 – 2241Substrate By similarity
Binding sitei245 – 2451Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarity
Nucleotide bindingi102 – 1054ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1594-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Synonyms:pfk
Ordered Locus Names:SA1521
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000751: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_0000111978Add
BLAST

2D gel databases

SWISS-2DPAGEiP99165.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi158879.SA1521.

Structurei

3D structure databases

ProteinModelPortaliP99165.
SMRiP99165. Positions 1-322.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni127 – 1293Substrate binding By similarity
Regioni171 – 1733Substrate binding By similarity
Regioni187 – 1893Allosteric activator ADP binding By similarity
Regioni215 – 2173Allosteric activator ADP binding By similarity
Regioni251 – 2544Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P99165-1 [UniParc]FASTAAdd to Basket

« Hide

MKKIAVLTSG GDSPGMNAAV RAVVRTAIYN EIEVYGVYHG YQGLLNDDIH    50
KLELGSVGDT IQRGGTFLYS ARCPEFKEQE VRKVAIENLR KRGIEGLVVI 100
GGDGSYRGAQ RISEECKEIQ TIGIPGTIDN DINGTDFTIG FDTALNTIIG 150
LVDKIRDTAS SHARTFIIEA MGRDCGDLAL WAGLSVGAET IVVPEVKTDI 200
KEIADKIEQG IKRGKKHSIV LVAEGCMTAQ DCQKELSQYI NVDNRVSVLG 250
HVQRGGSPTG ADRVLASRLG GYAVDLLMQG ETAKGVGIKN NKIVATSFDE 300
IFDGKDHKFD YSLYELANKL SI 322
Length:322
Mass (Da):34,840
Last modified:October 11, 2004 - v1
Checksum:i1FAE41C0BA7CA7F1
GO

Sequence cautioni

The sequence BAB42788.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000018 Genomic DNA. Translation: BAB42788.1. Different initiation.
PIRiG89953.
RefSeqiNP_374809.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB42788; BAB42788; BAB42788.
GeneIDi1124366.
KEGGisau:SA1521.
PATRICi19575342. VBIStaAur116463_1641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000018 Genomic DNA. Translation: BAB42788.1 . Different initiation.
PIRi G89953.
RefSeqi NP_374809.1. NC_002745.2.

3D structure databases

ProteinModelPortali P99165.
SMRi P99165. Positions 1-322.
ModBasei Search...

Protein-protein interaction databases

STRINGi 158879.SA1521.

2D gel databases

SWISS-2DPAGEi P99165.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB42788 ; BAB42788 ; BAB42788 .
GeneIDi 1124366.
KEGGi sau:SA1521.
PATRICi 19575342. VBIStaAur116463_1641.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
KOi K00850.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci SAUR158879:GJCB-1594-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: N315.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: N315.
  3. "Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315."
    Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.
    Submitted (OCT-2007) to UniProtKB
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: N315.

Entry informationi

Entry nameiPFKA_STAAN
AccessioniPrimary (citable) accession number: P99165
Secondary accession number(s): Q99TG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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