Reviewed, UniProtKB/Swiss-Prot P99162 (PUR3_STAAN)

Names and origin

Protein names

Recommended name:
    Phosphoribosylglycinamide formyltransferase
    EC=2.1.2.2
Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
      Short name=GART

Gene names

Name:	purN
Ordered Locus Names:	SA0924

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Phosphoribosylglycinamide formyltransferase		PRO_0000074947
Regions
	Region	12 – 14	3	5'-phosphoribosylglycinamide binding By similarity
	Region	91 – 94	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	110	1	Proton donor By similarity
	Binding site	66	1	10-formyltetrahydrofolate By similarity
	Binding site	108	1	10-formyltetrahydrofolate By similarity
	Binding site	146	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	175	1	5'-phosphoribosylglycinamide By similarity
	Site	146	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	P99162-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: F0364618F275FA30	FASTA	188	21,166
	10 20 30 40 50 60 MVKIAIFASG SGSNFENIVE HVESGKLENI EVTALYTDHQ NAFCIDRAKK HDIPVYINEP 70 80 90 100 110 120 KQFDSKAAYE QHLVTLLNKD KVEWIILAGY MRLIGPDLLA SFEGKILNIH PSLLPKYKGI 130 140 150 160 170 180 DAIGQAYHSG DTITGSTVHY VDCGMDTGEI IEQRQCDIRP DDSKEQLEEK VKKLEYELYP SVIAKIVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth." Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y., Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J. J. Microbiol. Methods 60:247-257(2005) [PubMed: 15590099] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[3]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000018 Genomic DNA. Translation: BAB42169.1.
PIR	F89876.
RefSeq	NP_374191.1.
3D structure databases
HSSP	HSSP built from PDB template 1GAR based on UniProtKB P08179.
ModBase	Search...
Protein-protein interaction databases
STRING	P99162.
2-D gel databases
SWISS-2DPAGE	P99162.
Genome annotation databases
GeneID	1123747.
GenomeReviews	Gene locus SA0924 in contig BA000018_GR.
KEGG	sau:SA0924.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P99162.
OMA	CSVHFVT.
Enzyme and pathway databases
BioCyc	SAUR158879:SA0924-MON.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_STAAN

Accession

Primary (citable) accession number: P99162
Secondary accession number(s): Q99V25

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)