ID   ALF1_STAAN              Reviewed;         296 AA.
AC   P99117; Q99R31;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   16-JUN-2009, entry version 28.
DE   RecName: Full=Fructose-bisphosphate aldolase class 1;
DE            EC=4.1.2.13;
DE   AltName: Full=Fructose-biphosphate aldolase class I;
DE            Short=FBP aldolase;
GN   Name=fda; OrderedLocusNames=SA2399;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=21311952; PubMed=11418146; DOI=10.1016/S0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I.,
RA   Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K.,
RA   Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M.,
RA   Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M.,
RA   Ogasawara N., Hayashi H., Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus
RT   aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=15590099; DOI=10.1016/j.mimet.2004.09.017;
RA   Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y.,
RA   Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D.,
RA   Sanchez J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F.,
RA   Schrenzel J.;
RT   "Correlation of proteomic and transcriptomic profiles of
RT   Staphylococcus aureus during the post-exponential phase of growth.";
RL   J. Microbiol. Methods 60:247-257(2005).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RA   Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT   "Shotgun proteomic analysis of total and membrane protein extracts of
RT   S. aureus strain N315.";
RL   Submitted (OCT-2007) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY: D-fructose 1,6-bisphosphate = glycerone
CC       phosphate + D-glyceraldehyde 3-phosphate.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 4/4.
CC   -!- SIMILARITY: Belongs to the class I fructose-bisphosphate aldolase
CC       family.
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DR   EMBL; BA000018; BAB43704.1; -; Genomic_DNA.
DR   PIR; F90067; F90067.
DR   RefSeq; NP_375725.1; -.
DR   HSSP; P00883; 1EWD.
DR   SWISS-2DPAGE; P99117; -.
DR   GeneID; 1125328; -.
DR   GenomeReviews; BA000018_GR; SA2399.
DR   KEGG; sau:SA2399; -.
DR   HOGENOM; P99117; -.
DR   OMA; P99117; GTKMRSV.
DR   BioCyc; SAUR158879:SA2399-MON; -.
DR   GO; GO:0004332; F:fructose-bisphosphate aldolase activity; IEA:HAMAP.
DR   GO; GO:0006096; P:glycolysis; IEA:HAMAP.
DR   HAMAP; MF_00729; -; 1.
DR   InterPro; IPR000741; Aldolase_I.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   PANTHER; PTHR11627; Aldolase_I; 1.
DR   Pfam; PF00274; Glycolytic; 1.
DR   ProDom; PD001128; Aldolase_I; 1.
DR   PROSITE; PS00158; ALDOLASE_CLASS_I; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Glycolysis; Lyase; Schiff base.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    296       Fructose-bisphosphate aldolase class 1.
FT                                /FTId=PRO_0000216905.
FT   ACT_SITE    175    175       Proton acceptor (By similarity).
FT   ACT_SITE    212    212       Schiff-base intermediate with
FT                                dihydroxyacetone-P (By similarity).
SQ   SEQUENCE   296 AA;  33042 MW;  D63B1A3C7646725B CRC64;
     MNKEQLEKMK NGKGFIAALD QSGGSTPKAL KEYGVNEDQY SNEDEMFQLV HDMRTRVVTS
     PSFSPDKILG AILFEQTMDR EVEGKYTADY LADKGVVPFL KVDKGLAEEQ NGVQLMKPID
     NLDSLLDRAN ERHIFGTKMR SNILELNEQG IKDVVEQQFE VAKQIIAKGL VPIIEPEVNI
     NAKDKAEIEK VLKAELKKGL DSLNADQLVM LKLTIPTEPN LYKELAEHPN VVRVVVLSGG
     YSREKANELL KDNDELIASF SRALASDLRA DQSKEEFDKA LGDAVESIYD ASVNKN
//