P99116 (LDHD_STAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-lactate dehydrogenase
Short name=D-LDH
EC=1.1.1.28

Alternative name(s):
D-specific D-2-hydroxyacid dehydrogenase

Gene names

Name:	ldhD
Synonyms:	ddh
Ordered Locus Names:	SA2312

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(R)-lactate + NAD+ = pyruvate + NADH.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
Sequence caution	The sequence BAB43615.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	D-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	D-lactate dehydrogenase		PRO_0000075961
Regions
	Nucleotide binding	156 – 157	2	NAD By similarity
	Nucleotide binding	233 – 235	3	NAD By similarity
	Nucleotide binding	296 – 299	4	NAD By similarity
Sites
	Active site	235	1	By similarity
	Active site	264	1	By similarity
	Active site	296	1	Proton donor By similarity
	Binding site	259	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P99116 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: AE23FA56A784B320	FASTA	330	36,712
	10 20 30 40 50 60 MTKIMFFGTR DYEKEMALNW GKKNNVEVTT SKELLSSATV DQLKDYDGVT TMQFGKLEND 70 80 90 100 110 120 VYPKLESYGI KQIAQRTAGF DMYDLDLAKK HNIVISNVPS YSPETIAEYS VSIALQLVRR 130 140 150 160 170 180 FPDIERRVQT HDFTWQAEIM SKPVKNMTVA IIGTGRIGAA TAKIYAGFGA TITAYDAYPN 190 200 210 220 230 240 KDLDFLTYKD SVKEAIKDAD IISLHVPANK ESYHLFDKAM FDHVKKGAIL VNAARGAVIN 250 260 270 280 290 300 TPDLIAAVND GTLLGAAIDT YENEAAYFTN DWTNKDIDDK TLLELIEHER ILVTPHIAFF 310 320 330 SDEAVQNLVE GGLNAALSVI NTGTCETRLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: N315.
[2]	"Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth." Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y., Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J. J. Microbiol. Methods 60:247-257(2005) [PubMed: 15590099] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: N315.
[3]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: N315.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000018 Genomic DNA. Translation: BAB43615.1. Different initiation.
PIR	E90056.
RefSeq	NP_375636.1. NC_002745.2.
3D structure databases
ProteinModelPortal	P99116.
ModBase	Search...
Protein-protein interaction databases
STRING	P99116.
2D gel databases
SWISS-2DPAGE	P99116.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000001918; EBSTAP00000001918; EBSTAG00000001918.
GeneID	1125239.
GenomeReviews	Gene locus SA2312 in contig BA000018_GR.
KEGG	sau:SA2312.
PATRIC	19577180. VBIStaAur116463_2508.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1052.
GeneTree	EBGT00050000024534.
HOGENOM	HBG731446.
ProtClustDB	PRK12480.
Enzyme and pathway databases
BioCyc	SAUR158879:SA2312-MONOMER.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K03778.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHD_STAAN

Accession

Primary (citable) accession number: P99116
Secondary accession number(s): Q99RB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program