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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathway: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891NADP; via carbonyl oxygenBy similarity
Binding sitei141 – 1411SubstrateBy similarity
Active sitei154 – 1541Proton acceptorPROSITE-ProRule annotation
Binding sitei187 – 1871NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 144NADPBy similarity
Nucleotide bindingi62 – 632NADPBy similarity
Nucleotide bindingi154 – 1585NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1134-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:SA1074
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2462463-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054685Add
BLAST

2D gel databases

SWISS-2DPAGEP99093.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP99093.
SMRiP99093. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK00059.
OMAiTGQELHV.
OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P99093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMTKSALVT GASRGIGRSI ALQLAEEGYN VAVNYAGSKE KAEAVVEEIK
60 70 80 90 100
AKGVDSFAIQ ANVADADEVK AMIKEVVSQF GSLDVLVNNA GITRDNLLMR
110 120 130 140 150
MKEQEWDDVI DTNLKGVFNC IQKATPQMLR QRSGAIINLS SVVGAVGNPG
160 170 180 190 200
QANYVATKAG VIGLTKSAAR ELASRGITVN AVAPGFIVSD MTDALSDELK
210 220 230 240
EQMLTQIPLA RFGQDTDIAN TVAFLASDKA KYITGQTIHV NGGMYM
Length:246
Mass (Da):26,146
Last modified:March 1, 2005 - v1
Checksum:i2BD7CA319D3A37C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85817 Genomic DNA. Translation: BAB20935.2.
BA000018 Genomic DNA. Translation: BAB42326.1.
PIRiB89896.
RefSeqiNP_374347.1. NC_002745.2.
WP_001790526.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB42326; BAB42326; BAB42326.
KEGGisau:SA1074.
PATRICi19574368. VBIStaAur116463_1153.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85817 Genomic DNA. Translation: BAB20935.2.
BA000018 Genomic DNA. Translation: BAB42326.1.
PIRiB89896.
RefSeqiNP_374347.1. NC_002745.2.
WP_001790526.1. NC_002745.2.

3D structure databases

ProteinModelPortaliP99093.
SMRiP99093. Positions 1-246.
ModBaseiSearch...
MobiDBiSearch...

2D gel databases

SWISS-2DPAGEP99093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB42326; BAB42326; BAB42326.
KEGGisau:SA1074.
PATRICi19574368. VBIStaAur116463_1153.

Phylogenomic databases

eggNOGiCOG1028.
KOiK00059.
OMAiTGQELHV.
OrthoDBiEOG6N3CR8.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciSAUR158879:GJCB-1134-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Eagle-type methicillin resistance: new phenotype of high methicillin resistance under mec regulator gene control."
    Kondo N., Kuwahara-Arai K., Kuroda-Murakami H., Tateda-Suzuki E., Hiramatsu K.
    Antimicrob. Agents Chemother. 45:815-824(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: N315.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: N315.
  4. "Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315."
    Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.
    Submitted (OCT-2007) to UniProtKB
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: N315.

Entry informationi

Entry nameiFABG_STAAN
AccessioniPrimary (citable) accession number: P99093
Secondary accession number(s): Q99QK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: June 24, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.