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P99085

- HPRT_STAAN

UniProt

P99085 - HPRT_STAAN

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

hpt

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei102 – 1021Proton acceptorBy similarity
    Binding sitei130 – 1301IMPBy similarity
    Binding sitei152 – 1521IMP; via carbonyl oxygenBy similarity
    Metal bindingi158 – 1581MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi98 – 10710IMPBy similarity
    Nucleotide bindingi157 – 1582IMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. IMP salvage Source: UniProtKB-UniPathway
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSAUR158879:GJCB-489-MONOMER.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:hpt
    Ordered Locus Names:SA0468
    OrganismiStaphylococcus aureus (strain N315)
    Taxonomic identifieri158879 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000751: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 179179Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139614Add
    BLAST

    Proteomic databases

    PRIDEiP99085.

    2D gel databases

    SWISS-2DPAGEP99085.

    Interactioni

    Protein-protein interaction databases

    STRINGi158879.SA0468.

    Structurei

    3D structure databases

    ProteinModelPortaliP99085.
    SMRiP99085. Positions 4-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    HOGENOMiHOG000236520.
    KOiK00760.
    OMAiVDFMTVS.
    OrthoDBiEOG693GNP.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P99085-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHNDLKEVLL TEEDIQNICK ELGAQLTKDY QGKPLVCVGI LKGSAMFMSD    50
    LIKRIDTHLS IDFMDVSSYH GGTESTGEVQ IIKDLGSSIE NKDVLIIEDI 100
    LETGTTLKSI TELLQSRKVN SLEIVTLLDK PNRRKADIEA KYVGKKIPDE 150
    FVVGYGLDYR ELYRNLPYIG TLKPEVYSN 179
    Length:179
    Mass (Da):20,154
    Last modified:October 11, 2004 - v1
    Checksum:iE7D35987B435EFB0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000018 Genomic DNA. Translation: BAB41698.1.
    PIRiG89817.
    RefSeqiNP_373720.1. NC_002745.2.

    Genome annotation databases

    EnsemblBacteriaiBAB41698; BAB41698; BAB41698.
    GeneIDi1123259.
    KEGGisau:SA0468.
    PATRICi19573020. VBIStaAur116463_0493.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000018 Genomic DNA. Translation: BAB41698.1 .
    PIRi G89817.
    RefSeqi NP_373720.1. NC_002745.2.

    3D structure databases

    ProteinModelPortali P99085.
    SMRi P99085. Positions 4-177.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158879.SA0468.

    2D gel databases

    SWISS-2DPAGE P99085.

    Proteomic databases

    PRIDEi P99085.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB41698 ; BAB41698 ; BAB41698 .
    GeneIDi 1123259.
    KEGGi sau:SA0468.
    PATRICi 19573020. VBIStaAur116463_0493.

    Phylogenomic databases

    eggNOGi COG0634.
    HOGENOMi HOG000236520.
    KOi K00760.
    OMAi VDFMTVS.
    OrthoDBi EOG693GNP.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci SAUR158879:GJCB-489-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: N315.
    2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: N315.

    Entry informationi

    Entry nameiHPRT_STAAN
    AccessioniPrimary (citable) accession number: P99085
    Secondary accession number(s): Q99W93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3