P99083 (CH60_STAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60 kDa chaperonin

Alternative name(s):
GroEL protein
Protein Cpn60

Gene names

Name:	groL
Synonyms:	groEL
Ordered Locus Names:	SA1836

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions By similarity. HAMAP-Rule MF_00600
Subunit structure	Oligomer of 14 subunits composed of two stacked rings of 7 subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the chaperonin (HSP60) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein refolding Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	60 kDa chaperonin HAMAP-Rule MF_00600		PRO_0000063533

Sequences

	Sequence		Length	Mass (Da)
	P99083 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: A3D8595603DC9313	FASTA	538	57,572
	10 20 30 40 50 60 MVKQLKFSED ARQAMLRGVD QLANAVKVTI GPKGRNVVLD KEFTAPLITN DGVTIAKEIE 70 80 90 100 110 120 LEDPYENMGA KLVQEVANKT NEIAGDGTTT ATVLAQAMIQ EGLKNVTSGA NPVGLRQGID 130 140 150 160 170 180 KAVKVAVEAL HENSQKVENK NEIAQVGAIS AADEEIGRYI SEAMEKVGND GVITIEESNG 190 200 210 220 230 240 LNTELEVVEG MQFDRGYQSP YMVTDSDKMV AELERPYILV TDKKISSFQD ILPLLEQVVQ 250 260 270 280 290 300 SNRPILIVAD EVEGDALTNI VLNRMRGTFT AVAVKAPGFG DRRKAMLEDL AILTGAQVIT 310 320 330 340 350 360 DDLGLDLKDA SIDMLGTASK VEVTKDNTTV VDGDGDENSI DARVSQLKSQ IEETESDFDR 370 380 390 400 410 420 EKLQERLAKL AGGVAVIKVG AASETELKER KLRIEDALNS TRAAVEEGIV AGGGTALVNV 430 440 450 460 470 480 YQKVSEIEAE GDIETGVNIV LKALTAPVRQ IAENAGLEGS VIVERLKNAE PGVGFNAATN 490 500 510 520 530 EWVNMLEAGI VDPTKVTRSA LQHAASVAAM FLTTEAVVAS IPEKNNDQPN MGGMPGMM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: N315.
[2]	"Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth." Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y., Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J. J. Microbiol. Methods 60:247-257(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: N315.
[3]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: N315.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000018 Genomic DNA. Translation: BAB43116.1.
PIR	C89994.
RefSeq	NP_375137.1. NC_002745.2.
3D structure databases
ProteinModelPortal	P99083.
SMR	P99083. Positions 3-524.
ModBase	Search...
Protein-protein interaction databases
STRING	158879.SA1836.
2D gel databases
SWISS-2DPAGE	P99083.
Proteomic databases
PRIDE	P99083.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB43116; BAB43116; BAB43116.
GeneID	1124728.
KEGG	sau:SA1836.
PATRIC	19576110. VBIStaAur116463_1987.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0459.
HOGENOM	HOG000076290.
KO	K04077.
OMA	YNGVITV.
ProtClustDB	PRK00013.
Enzyme and pathway databases
BioCyc	SAUR158879:GJCB-1960-MONOMER.
Family and domain databases
HAMAP	MF_00600. CH60.
InterPro	IPR018370. Chaperonin_Cpn60_CS. IPR001844. Chaprnin_Cpn60. IPR002423. Cpn60/TCP-1. [Graphical view]
PANTHER	PTHR11353. PTHR11353. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00298. CHAPERONIN60.
SUPFAM	SSF48592. GroEL-ATPase. 1 hit.
TIGRFAMs	TIGR02348. GroEL. 1 hit.
PROSITE	PS00296. CHAPERONINS_CPN60. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CH60_STAAN

Accession

Primary (citable) accession number: P99083
Secondary accession number(s): Q99SL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program